Restriction map for
pFN21AB7686
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BbeI BbrPI BbsI
BbvCI BciVI BclI BfaI BfrI
BglII BlnI BpuAI BsaBI BsaWI
BseAI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI Eam1105I Eco47III Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MamI MfeI MroI MseI MunI
NarI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI SspBI SwaI TfiI Tru9I
Tth111I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AcyI 346 GACGCC G[GA]^CG[CT]C
AfaI 223 GTAC GT^AC
AflIII 898 ACGCGT A^C[GA][CT]GT
AlwNI 35 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 474 GGGCCC GGGCC^C
Asp700I 775 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BcgI 843 CGAGGCCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 467 GCCCACGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1021 CACGTA [CT]AC^GT[GA]
BsaHI 346 GACGCC G[GA]^CG[CT]C
BsgI 352 GTGCAG GTGCAG(16/14)
BsiEI 424 CGGCCG CG[GA][CT]^CG
BsiWI 222 CGTACG C^GTACG
BspMI 710 ACCTGC ACCTGC(4/8)
BsrDI 596 GCAATG GCAATG(2/0)
EagI 424 CGGCCG C^GGCCG
EclXI 424 CGGCCG C^GGCCG
Eco52I 424 CGGCCG C^GGCCG
EcoNI 99 CCTGGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 974 CCTTGG C^C[AT][AT]GG
HaeII 362 AGCGCC [GA]GCGC^[CT]
Hin1I 346 GACGCC G[GA]^CG[CT]C
MaeII 1022 ACGT A^CGT
MaeIII 589 GTCAC ^GT[ACGT]AC
MluI 898 ACGCGT A^CGCGT
RsaI 223 GTAC GT^AC
SspI 580 AATATT AAT^ATT
StyI 974 CCTTGG C^C[AT][AT]GG
TaqI 1157 TCGA T^CGA
Tsp45I 589 GTCAC ^GT[GC]AC
Tsp509I 1109 AATT ^AATT
TthHB8I 1157 TCGA T^CGA
XmnI 775 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AvaI 787 CTCGGG C^[CT]CG[GA]G
841 CCCGAG
BalI 521 TGGCCA TGG^CCA
884 TGGCCA
BamHI 108 GGATCC G^GATCC
1011 GGATCC
BlpI 550 GCTAAGC GC^T[ACGT]AGC
1056 GCTGAGC
Bpu1102I 550 GCTAAGC GC^T[ACGT]AGC
1056 GCTGAGC
BsmBI 895 GAGACG CGTCTC(1/5)
994 GAGACG
BsoBI 787 CTCGGG C^[CT]CG[GA]G
841 CCCGAG
BstAPI 191 GCAACGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
290 GCAACCAGTGC
BstXI 85 CCAGGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
497 CCAGAGCCCTGG
BstYI 108 GGATCC [GA]^GATC[CT]
1011 GGATCC
CelII 550 GCTAAGC GC^T[ACGT]AGC
1056 GCTGAGC
HphI 747 GGTGA GGTGA(8/7)
590 TCACC
MflI 108 GGATCC [GA]^GATC[CT]
1011 GGATCC
MluNI 521 TGGCCA TGG^CCA
884 TGGCCA
MscI 521 TGGCCA TGG^CCA
884 TGGCCA
MslI 114 CACAGGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
480 CACACCCATG
NaeI 308 GCCGGC GCC^GGC
872 GCCGGC
NgoMIV 308 GCCGGC G^CCGGC
872 GCCGGC
PflMI 513 CCACTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
794 CCATCCAGTGG
PshAI 817 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
897 GACGCGTGTC
SacI 27 GAGCTC GAGCT^C
1159 GAGCTC
SbfI 324 CCTGCAGG CCTGCA^GG
906 CCTGCAGG
Sse8387I 324 CCTGCAGG CCTGCA^GG
906 CCTGCAGG
Van91I 513 CCACTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
794 CCATCCAGTGG
XhoII 108 GGATCC [GA]^GATC[CT]
1011 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AccII AciI AluI AlwI AspHI
AvaII BanI BanII BbvI BcnI
BmyI BpmI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII ItaI
Ksp632I MboI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
PvuII Sau3AI Sau96I ScrFI SfaNI
SfcI StuI TseI TspRI