Restriction map for
pFN21AE2092
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# NO CUT ENZYMES ( 82 enzymes) #
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AccIII AclI AflII AgeI Alw44I
ApaLI AscI AseI AsnI AspI
AviII AvrII BbeI BciVI BclI
BfrI BlnI BsaWI BseAI BsiWI
Bsp1407I BspEI BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstZ17I CpoI
DraI DraIII DrdI EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KasI MfeI
MluI MroI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SalI SapI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SspBI SspI SwaI Tth111I
XbaI XhoI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 1653 GACGTC GACGT^C
Acc65I 1766 GGTACC G^GTACC
AccI 1315 GTCTAC GT^[AC][GT]AC
AcyI 1653 GACGTC G[GA]^CG[CT]C
AflIII 1820 ACATGT A^C[GA][CT]GT
AocI 2227 CCTGAGG CC^T[ACGT]AGG
ApaI 2322 GGGCCC GGGCC^C
Asp718I 1766 GGTACC G^GTACC
BalI 248 TGGCCA TGG^CCA
BbrPI 2169 CACGTG CAC^GTG
BglI 1673 GCCACGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1493 AGATCT A^GATCT
BlpI 1668 GCTGAGC GC^T[ACGT]AGC
BpmI 690 CTGGAG CTGGAG(16/14)
Bpu1102I 1668 GCTGAGC GC^T[ACGT]AGC
BsaAI 2169 CACGTG [CT]AC^GT[GA]
BsaBI 1494 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1653 GACGTC G[GA]^CG[CT]C
BsmI 477 GCATTC GAATGC(1/-1)
BspDI 1891 ATCGAT AT^CGAT
BspHI 2057 TCATGA T^CATGA
BspLU11I 1820 ACATGT A^CATGT
BstAPI 1218 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1755 GGTGACC G^GT[ACGT]ACC
BstPI 1755 GGTGACC G^GT[ACGT]ACC
BstXI 1808 CCACGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2227 CCTGAGG CC^T[ACGT]AGG
CelII 1668 GCTGAGC GC^T[ACGT]AGC
ClaI 1891 ATCGAT AT^CGAT
Eco81I 2227 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1755 GGTGACC G^GT[ACGT]ACC
EcoT14I 1796 CCAAGG C^C[AT][AT]GG
HgaI 1262 GCGTC GACGC(5/10)
Hin1I 1653 GACGTC G[GA]^CG[CT]C
KpnI 1766 GGTACC GGTAC^C
MamI 1494 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 248 TGGCCA TGG^CCA
MscI 248 TGGCCA TGG^CCA
MseI 103 TTAA T^TAA
PmaCI 2169 CACGTG CAC^GTG
PmlI 2169 CACGTG CAC^GTG
PstI 1246 CTGCAG CTGCA^G
RcaI 2057 TCATGA T^CATGA
SbfI 1245 CCTGCAGG CCTGCA^GG
SphI 360 GCATGC GCATG^C
Sse8387I 1245 CCTGCAGG CCTGCA^GG
StyI 1796 CCAAGG C^C[AT][AT]GG
Tru9I 103 TTAA T^TAA
XcmI 1593 CCAGCTGCTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 29 enzymes) #
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AcsI 44 AAATTC [GA]^AATT[CT]
108 AAATTC
AhdI 781 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1819 GACATGTGGTC
Aor51HI 2018 AGCGCT AGC^GCT
2098 AGCGCT
ApoI 44 AAATTC [GA]^AATT[CT]
108 AAATTC
Asp700I 43 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2061 GAACAAGTTC
AspEI 781 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1819 GACATGTGGTC
BamHI 1631 GGATCC G^GATCC
1723 GGATCC
BbvCI 1407 GCTGAGG CCTCAGC(-5/-2)
2101 GCTGAGG
BfaI 365 CTAG C^TAG
1931 CTAG
BsmBI 737 GAGACG CGTCTC(1/5)
2275 GAGACG
Eam1105I 781 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1819 GACATGTGGTC
Eco47III 2018 AGCGCT AGC^GCT
2098 AGCGCT
KspI 715 CCGCGG CCGC^GG
2318 CCGCGG
MaeI 365 CTAG C^TAG
1931 CTAG
MslI 1500 CATCGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1809 CACGGCCGTG
NaeI 1239 GCCGGC GCC^GGC
1678 GCCGGC
NgoMIV 1239 GCCGGC G^CCGGC
1678 GCCGGC
NspI 360 GCATGC [GA]CATG^[CT]
1820 ACATGT
PflMI 683 CCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1062 CCACCTCTTGG
PpuMI 779 GGGACCT [GA]G^G[AT]CC[CT]
1924 GGGACCC
PvuII 1594 CAGCTG CAG^CTG
2251 CAGCTG
SacI 1210 GAGCTC GAGCT^C
1960 GAGCTC
SacII 715 CCGCGG CCGC^GG
2318 CCGCGG
SmaI 1876 CCCGGG CCC^GGG
2083 CCCGGG
StuI 1736 AGGCCT AGG^CCT
2235 AGGCCT
Van91I 683 CCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1062 CCACCTCTTGG
XmaCI 1876 CCCGGG C^CCGGG
2083 CCCGGG
XmaI 1876 CCCGGG C^CCGGG
2083 CCCGGG
XmnI 43 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2061 GAACAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BanI
BanII BbsI BbvI BcgI BcnI
BmyI BpuAI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EarI EclXI
Eco52I EcoNI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII