Restriction map for
pFN21AE3066
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# NO CUT ENZYMES ( 169 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AvaI AviII AvrII BalI BamHI
BanII BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsiYI
BslI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstF5I BstPI BstUI
BstYI Bsu36I Cac8I CelII ClaI
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FokI FseI FspI
HaeIII HgaI KpnI Ksp632I KspI
MamI MfeI MflI MluI MluNI
MroI MscI MslI MspA1I MunI
MvnI MwoI NaeI NcoI NgoMIV
NheI NlaIII NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI TfiI
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccI 321 GTATAC GT^[AC][GT]AC
AcyI 61 GGCGCC G[GA]^CG[CT]C
AfaI 120 GTAC GT^AC
AflIII 88 ACGTGT A^C[GA][CT]GT
AlwI 536 GGATC GGATC(4/5)
AlwNI 361 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 254 GAGCAC G[AT]GC[AT]^C
AspI 490 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 27 GGACC G^G[AT]CC
BanI 61 GGCGCC G^G[CT][GA]CC
BbeI 61 GGCGCC GGCGC^C
BbvI 520 GCTGC GCAGC(8/12)
BcnI 68 CCGGG CC^[GC]GG
BfaI 638 CTAG C^TAG
BmyI 254 GAGCAC G[AGT]GC[ACT]^C
BsaAI 87 TACGTG [CT]AC^GT[GA]
BsaHI 61 GGCGCC G[GA]^CG[CT]C
BsgI 653 GTGCAG GTGCAG(16/14)
BsiHKAI 254 GAGCAC G[AT]GC[AT]^C
BsmFI 56 GGGAC GGGAC(10/14)
Bsp1286I 254 GAGCAC G[AGT]GC[ACT]^C
BsrDI 164 CATTGC GCAATG(2/0)
BsrFI 29 ACCGGC [GA]^CCGG[CT]
Bst1107I 321 GTATAC GTA^TAC
BstXI 444 CCAGGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 321 GTATAC GTA^TAC
CfoI 62 GCGC GCG^C
Cfr10I 29 ACCGGC [GA]^CCGG[CT]
Cfr13I 27 GGACC G^G[ACGT]CC
DpnII 537 GATC ^GATC
Fnu4HI 520 GCTGC GC^[ACGT]GC
HaeII 61 GGCGCC [GA]GCGC^[CT]
HhaI 62 GCGC GCG^C
Hin1I 61 GGCGCC G[GA]^CG[CT]C
HinP1I 62 GCGC G^CGC
HincII 628 GTTAAC GT[CT]^[GA]AC
HindII 628 GTTAAC GT[CT]^[GA]AC
HindIII 297 AAGCTT A^AGCTT
HpaI 628 GTTAAC GTT^AAC
ItaI 520 GCTGC GC^[ACGT]GC
KasI 61 GGCGCC G^GCGCC
MaeI 638 CTAG C^TAG
MaeII 88 ACGT A^CGT
MboI 537 GATC ^GATC
NarI 61 GGCGCC GG^CGCC
NciI 68 CCGGG CC^[GC]GG
NdeI 403 CATATG CA^TATG
NdeII 537 GATC ^GATC
PflFI 490 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsaI 120 GTAC GT^AC
Sau3AI 537 GATC ^GATC
Sau96I 27 GGACC G^G[ACGT]CC
SfcI 131 CTATAG C^T[GA][CT]AG
SfoI 61 GGCGCC GGC^GCC
SmlI 98 CTCAAG C^T[CT][GA]AG
TseI 520 GCTGC G^C[AT]GC
Tsp45I 567 GTCAC ^GT[GC]AC
TspRI 365 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 490 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AcsI 194 AAATTT [GA]^AATT[CT]
306 GAATTT
AluI 298 AGCT AG^CT
643 AGCT
ApoI 194 AAATTT [GA]^AATT[CT]
306 GAATTT
BbsI 72 GAAGAC GAAGAC(2/6)
496 GTCTTC
BpuAI 72 GAAGAC GAAGAC(2/6)
496 GTCTTC
BsaJI 374 CCTTGG C^C[ACGT][ACGT]GG
531 CCTTGG
BsmAI 488 GAGAC GTCTC(1/5)
472 GTCTC
BsrI 138 ACTGG ACTGG(1/-1)
366 ACTGG
EcoT14I 374 CCTTGG C^C[AT][AT]GG
531 CCTTGG
HapII 30 CCGG C^CGG
68 CCGG
HpaII 30 CCGG C^CGG
68 CCGG
HphI 330 GGTGA GGTGA(8/7)
579 GGTGA
MaeIII 184 GTTAC ^GT[ACGT]AC
567 GTCAC
MspI 30 CCGG C^CGG
68 CCGG
NlaIV 61 GGCGCC GG[ACGT]^[ACGT]CC
609 GGAACC
StyI 374 CCTTGG C^C[AT][AT]GG
531 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
BseRI BssKI BstNI DdeI EcoRII
HinfI MboII MnlI MseI MvaI
PleI ScrFI Tru9I Tsp509I