Restriction map for
pFN21AE9534
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI AhdI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AvrII BamHI
BbrPI BbsI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrGI BsrI BssSI
Bst1107I BstBI BstEII BstPI BstYI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI Eam1105I Eco47III EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HincII HindII HindIII HpaI HphI
KpnI KspI MaeI MaeIII MamI
MfeI MflI MluI MseI MunI
NaeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfuI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tru9I Tsp45I Tsp509I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccIII 575 TCCGGA T^CCGGA
AlwI 473 GGATC GGATC(4/5)
AlwNI 711 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 708 CCTCAGG CC^T[ACGT]AGG
ApaI 219 GGGCCC GGGCC^C
AspI 493 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 84 CTCGGG C^[CT]CG[GA]G
BbeI 140 GGCGCC GGCGC^C
BbvCI 315 CCTCAGC CCTCAGC(-5/-2)
BcgI 827 GCATCATGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 917 GCCCGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 575 TCCGGA [AT]^CCGG[AT]
BseAI 575 TCCGGA T^CCGGA
BseRI 657 CTCCTC GAGGAG(10/8)
BsgI 763 GTGCAG GTGCAG(16/14)
BsoBI 84 CTCGGG C^[CT]CG[GA]G
BspEI 575 TCCGGA T^CCGGA
BsrBI 691 CCGCTC CCGCTC(-3/-3)
BsrDI 297 CATTGC GCAATG(2/0)
BstAPI 639 GCACCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 20 CCACCGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 708 CCTCAGG CC^T[ACGT]AGG
Eco81I 708 CCTCAGG CC^T[ACGT]AGG
EcoNI 627 CCTCTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 140 GGCGCC [GA]GCGC^[CT]
KasI 140 GGCGCC G^GCGCC
MroI 575 TCCGGA T^CCGGA
MslI 402 CATCACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 381 CAGCTG C[AC]G^C[GT]G
NarI 140 GGCGCC GG^CGCC
NcoI 662 CCATGG C^CATGG
NdeI 542 CATATG CA^TATG
PflFI 493 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 381 CAGCTG CAG^CTG
SfoI 140 GGCGCC GGC^GCC
SgrAI 770 CGCCGGTG C[GA]^CCGG[CT]G
TaqI 476 TCGA T^CGA
TspRI 405 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 493 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 476 TCGA T^CGA
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflIII 452 ACGTGT A^C[GA][CT]GT
803 ACACGT
AviII 64 TGCGCA TGC^GCA
125 TGCGCA
BalI 98 TGGCCA TGG^CCA
293 TGGCCA
BanII 219 GGGCCC G[GA]GC[CT]^C
915 GAGCCC
BpmI 481 CTGGAG CTGGAG(16/14)
717 CTGGAG
BsiEI 179 CGGCCG CG[GA][CT]^CG
758 CGGCCG
BspMI 122 ACCTGC ACCTGC(4/8)
170 ACCTGC
BsrFI 617 ACCGGC [GA]^CCGG[CT]
771 GCCGGT
Cfr10I 617 ACCGGC [GA]^CCGG[CT]
771 GCCGGT
DpnII 352 GATC ^GATC
474 GATC
DraII 389 GGGTCCC [GA]G^G[ACGT]CC[CT]
979 GGGACCC
EagI 179 CGGCCG C^GGCCG
758 CGGCCG
EclXI 179 CGGCCG C^GGCCG
758 CGGCCG
Eco52I 179 CGGCCG C^GGCCG
758 CGGCCG
EcoO109I 389 GGGTCCC [GA]G^G[ACGT]CC[CT]
979 GGGACCC
FspI 64 TGCGCA TGC^GCA
125 TGCGCA
HgaI 442 GACGC GACGC(5/10)
768 GACGC
HinfI 961 GAATC G^A[ACGT]TC
970 GAATC
MaeII 452 ACGT A^CGT
805 ACGT
MboI 352 GATC ^GATC
474 GATC
MluNI 98 TGGCCA TGG^CCA
293 TGGCCA
MscI 98 TGGCCA TGG^CCA
293 TGGCCA
NdeII 352 GATC ^GATC
474 GATC
PpuMI 389 GGGTCCC [GA]G^G[AT]CC[CT]
979 GGGACCC
PstI 205 CTGCAG CTGCA^G
241 CTGCAG
Sau3AI 352 GATC ^GATC
474 GATC
SfcI 205 CTGCAG C^T[GA][CT]AG
241 CTGCAG
TfiI 961 GAATC G^A[AT]TC
970 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaII BanI BbvI BcnI
BmyI BsaHI BsaJI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BssHII BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DsaI EaeI EarI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI Hin1I HinP1I
HpaII ItaI Ksp632I MboII MnlI
MspI MvaI MvnI MwoI NciI
NlaIII NlaIV RsaI SapI Sau96I
ScrFI SfaNI StyI TseI