Restriction map for
pFN21AE1595
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# NO CUT ENZYMES ( 172 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI AlwNI Aor51HI ApaI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbvCI BcgI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsiEI BsiWI BsmAI BsmBI
BsmI BsoBI BspDI BspEI BspLU11I
BspMI BsrBI BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstF5I BstPI
BstUI BstXI BstYI BstZ17I CelII
CfoI Cfr10I Cfr13I ClaI CpoI
DraI DraII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FokI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HindIII HpaI KasI KpnI KspI
MaeIII MamI MfeI MflI MluI
MluNI MroI MscI MslI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI Sau96I
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I StyI SwaI
TaqI Tsp45I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AcsI 953 AAATTT [GA]^AATT[CT]
Alw44I 654 GTGCAC G^TGCAC
AlwI 3 GGATC GGATC(4/5)
ApaLI 654 GTGCAC G^TGCAC
ApoI 953 AAATTT [GA]^AATT[CT]
BbsI 1002 GAAGAC GAAGAC(2/6)
BclI 878 TGATCA T^GATCA
BfaI 707 CTAG C^TAG
BpuAI 1002 GAAGAC GAAGAC(2/6)
BsaJI 620 CCACGG C^C[ACGT][ACGT]GG
BsgI 380 CTGCAC GTGCAG(16/14)
BsiYI 385 CCCTTGCCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 385 CCCTTGCCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 312 GTCCC GGGAC(10/14)
Bsp1407I 714 TGTACA T^GTACA
BspHI 596 TCATGA T^CATGA
BsrDI 540 CATTGC GCAATG(2/0)
BsrGI 714 TGTACA T^GTACA
BstAPI 544 GCACTTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 545 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 620 CCACGG C^C[GA][CT]GG
FbaI 878 TGATCA T^GATCA
HincII 306 GTCAAC GT[CT]^[GA]AC
HindII 306 GTCAAC GT[CT]^[GA]AC
HphI 818 TCACC GGTGA(8/7)
MaeI 707 CTAG C^TAG
MaeII 310 ACGT A^CGT
PstI 290 CTGCAG CTGCA^G
RcaI 596 TCATGA T^CATGA
SfaNI 93 GATGC GCATC(5/9)
SspBI 714 TGTACA T^GTACA
SspI 493 AATATT AAT^ATT
StuI 807 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 29 enzymes) #
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AfaI 478 GTAC GT^AC
715 GTAC
AocI 49 CCTCAGG CC^T[ACGT]AGG
335 CCTCAGG
AspHI 654 GTGCAC G[AT]GC[AT]^C
814 GTGCTC
BciVI 408 GTATCC GTATCC(6/5)
499 GTATCC
BcnI 67 CCCGG CC^[GC]GG
391 CCGGG
BsiHKAI 654 GTGCAC G[AT]GC[AT]^C
814 GTGCTC
BsrI 106 ACTGG ACTGG(1/-1)
687 ACTGG
BstNI 342 CCAGG CC^[AT]GG
859 CCTGG
Bsu36I 49 CCTCAGG CC^T[ACGT]AGG
335 CCTCAGG
DpnII 4 GATC ^GATC
879 GATC
EarI 355 CTCTTC CTCTTC(1/4)
157 GAAGAG
Eco81I 49 CCTCAGG CC^T[ACGT]AGG
335 CCTCAGG
EcoRII 342 CCAGG ^CC[AT]GG
859 CCTGG
HapII 68 CCGG C^CGG
391 CCGG
HpaII 68 CCGG C^CGG
391 CCGG
Ksp632I 355 CTCTTC CTCTTC(1/4)
157 GAAGAG
MboI 4 GATC ^GATC
879 GATC
MseI 865 TTAA T^TAA
928 TTAA
MspA1I 287 CAGCTG C[AC]G^C[GT]G
484 CAGCTG
MspI 68 CCGG C^CGG
391 CCGG
MvaI 342 CCAGG CC^[AT]GG
859 CCTGG
NciI 67 CCCGG CC^[GC]GG
391 CCGGG
NdeII 4 GATC ^GATC
879 GATC
PvuII 287 CAGCTG CAG^CTG
484 CAGCTG
RsaI 478 GTAC GT^AC
715 GTAC
SapI 157 GAAGAGC GCTCTTC(1/4)
354 GCTCTTC
Sau3AI 4 GATC ^GATC
879 GATC
SfcI 290 CTGCAG C^T[GA][CT]AG
870 CTACAG
Tru9I 865 TTAA T^TAA
928 TTAA
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# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AciI AluI BanI BbvI BmyI
Bsp1286I BssKI Cac8I DdeI Fnu4HI
HaeIII HinfI ItaI MboII MnlI
MwoI NlaIII NlaIV PleI ScrFI
TfiI TseI Tsp509I TspRI