Restriction map for
pFN21AE3808
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# NO CUT ENZYMES ( 131 enzymes) #
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AatII Acc65I AccI AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII BalI BbeI BbrPI
BcgI BciVI BclI BfrI BglII
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsiWI BsmI Bsp1407I BspDI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstZ17I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco47III EcoRI EcoRV EcoT22I FbaI
FseI FspI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MluNI MscI
MseI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NlaIII NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tsp509I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccIII 137 TCCGGA T^CCGGA
AlwNI 304 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 517 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 871 CCCGAG C^[CT]CG[GA]G
AvrII 540 CCTAGG C^CTAGG
BamHI 867 GGATCC G^GATCC
BanI 609 GGCACC G^G[CT][GA]CC
BbsI 511 GTCTTC GAAGAC(2/6)
BlnI 540 CCTAGG C^CTAGG
BpuAI 511 GTCTTC GAAGAC(2/6)
BsaWI 137 TCCGGA [AT]^CCGG[AT]
BseAI 137 TCCGGA T^CCGGA
BsgI 284 CTGCAC GTGCAG(16/14)
BsiEI 162 CGGCCG CG[GA][CT]^CG
BsmBI 279 CGTCTC CGTCTC(1/5)
BsoBI 871 CCCGAG C^[CT]CG[GA]G
BspEI 137 TCCGGA T^CCGGA
BstXI 805 CCACCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 867 GGATCC [GA]^GATC[CT]
DpnII 868 GATC ^GATC
EagI 162 CGGCCG C^GGCCG
EarI 694 CTCTTC CTCTTC(1/4)
EclXI 162 CGGCCG C^GGCCG
Eco52I 162 CGGCCG C^GGCCG
EcoNI 248 CCTTCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 964 AGCGCC [GA]GCGC^[CT]
HinfI 670 GACTC G^A[ACGT]TC
Ksp632I 694 CTCTTC CTCTTC(1/4)
MboI 868 GATC ^GATC
MflI 867 GGATCC [GA]^GATC[CT]
MroI 137 TCCGGA T^CCGGA
MslI 481 CACGCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII 868 GATC ^GATC
PflFI 517 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 66 CCAACGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 670 GACTC GAGTC(4/5)
PshAI 547 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 200 CAGCTG CAG^CTG
Sau3AI 868 GATC ^GATC
SfaNI 384 GATGC GCATC(5/9)
SmlI 999 CTCAAG C^T[CT][GA]AG
Tth111I 517 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 66 CCAACGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 802 CCACCACCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 867 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccII 166 CGCG CG^CG
360 CGCG
AfaI 126 GTAC GT^AC
357 GTAC
AlwI 868 GATCC GGATC(4/5)
867 GGATC
AocI 25 CCTGAGG CC^T[ACGT]AGG
252 CCTGAGG
BglI 433 GCCCAGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
651 GCCTCTGCGGC
BpmI 847 CTCCAG CTGGAG(16/14)
1006 CTGGAG
BsaI 19 GAGACC GGTCTC(1/5)
545 GAGACC
BstEII 96 GGTGACC G^GT[ACGT]ACC
774 GGTGACC
BstPI 96 GGTGACC G^GT[ACGT]ACC
774 GGTGACC
BstUI 166 CGCG CG^CG
360 CGCG
Bsu36I 25 CCTGAGG CC^T[ACGT]AGG
252 CCTGAGG
DsaI 605 CCACGG C^C[GA][CT]GG
665 CCGTGG
Eco81I 25 CCTGAGG CC^T[ACGT]AGG
252 CCTGAGG
EcoO65I 96 GGTGACC G^GT[ACGT]ACC
774 GGTGACC
EcoT14I 540 CCTAGG C^C[AT][AT]GG
721 CCTTGG
HgaI 222 GCGTC GACGC(5/10)
278 GCGTC
MaeII 503 ACGT A^CGT
728 ACGT
MaeIII 97 GTGAC ^GT[ACGT]AC
775 GTGAC
MboII 512 TCTTC GAAGA(8/7)
695 TCTTC
MvnI 166 CGCG CG^CG
360 CGCG
RsaI 126 GTAC GT^AC
357 GTAC
StuI 294 AGGCCT AGG^CCT
758 AGGCCT
StyI 540 CCTAGG C^C[AT][AT]GG
721 CCTTGG
TaqI 515 TCGA T^CGA
755 TCGA
Tsp45I 97 GTGAC ^GT[GC]AC
775 GTGAC
TthHB8I 515 TCGA T^CGA
755 TCGA
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# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AluI AspHI AvaII BanII
BbvCI BbvI BcnI BfaI BmyI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DraII EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MaeI MnlI MspA1I MspI MvaI
MwoI NciI NlaIV PpuMI PstI
SacI Sau96I ScrFI SfcI TseI
TspRI