Restriction map for
pFN21AE3762
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII AccI AcyI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp700I AspEI
AvrII BamHI BbeI BbrPI BcgI
BciVI BclI BfrI BglII BlnI
BsaAI BsaHI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspHI BspLU11I BspMI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstXI BstZ17I ClaI
CpoI DraI DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
Hin1I HincII HindII HpaI KasI
KspI MfeI MluI MunI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StyI SwaI XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
###################################
Acc65I 145 GGTACC G^GTACC
AccII 712 CGCG CG^CG
AclI 899 AACGTT AA^CGTT
AcsI 691 GAATTT [GA]^AATT[CT]
AlwI 828 GATCC GGATC(4/5)
AocI 1125 CCTGAGG CC^T[ACGT]AGG
ApoI 691 GAATTT [GA]^AATT[CT]
Asp718I 145 GGTACC G^GTACC
AspHI 1037 GAGCAC G[AT]GC[AT]^C
AspI 1618 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1178 TGCGCA TGC^GCA
BalI 113 TGGCCA TGG^CCA
BbsI 1517 GTCTTC GAAGAC(2/6)
BbvCI 1169 CCTCAGC CCTCAGC(-5/-2)
BglI 9 GCCCCCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1517 GTCTTC GAAGAC(2/6)
BsaBI 814 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 1037 GAGCAC G[AT]GC[AT]^C
BsmI 697 GAATGC GAATGC(1/-1)
BsrFI 305 ACCGGC [GA]^CCGG[CT]
BstUI 712 CGCG CG^CG
BstYI 827 AGATCC [GA]^GATC[CT]
Bsu36I 1125 CCTGAGG CC^T[ACGT]AGG
Cfr10I 305 ACCGGC [GA]^CCGG[CT]
DraIII 645 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 113 TGGCCA [CT]^GGCC[GA]
EarI 969 GAAGAG CTCTTC(1/4)
Eco81I 1125 CCTGAGG CC^T[ACGT]AGG
FspI 1178 TGCGCA TGC^GCA
HaeII 1100 AGCGCC [GA]GCGC^[CT]
HgaI 1526 GACGC GACGC(5/10)
HindIII 490 AAGCTT A^AGCTT
KpnI 145 GGTACC GGTAC^C
Ksp632I 969 GAAGAG CTCTTC(1/4)
MamI 814 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 827 AGATCC [GA]^GATC[CT]
MluNI 113 TGGCCA TGG^CCA
MscI 113 TGGCCA TGG^CCA
MvnI 712 CGCG CG^CG
NdeI 503 CATATG CA^TATG
PflFI 1618 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 370 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1583 GAGTC GAGTC(4/5)
PshAI 288 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 899 AACGTT AA^CGTT
PvuII 315 CAGCTG CAG^CTG
SexAI 1670 ACCTGGT A^CC[AT]GGT
SmaI 470 CCCGGG CCC^GGG
TaqI 1474 TCGA T^CGA
TfiI 202 GATTC G^A[AT]TC
Tsp45I 1416 GTCAC ^GT[GC]AC
Tth111I 1618 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1474 TCGA T^CGA
Van91I 370 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 149 CCATCAAAAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 827 AGATCC [GA]^GATC[CT]
XmaCI 470 CCCGGG C^CCGGG
XmaI 470 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccIII 731 TCCGGA T^CCGGA
1343 TCCGGA
BfaI 170 CTAG C^TAG
1523 CTAG
BlpI 18 GCTGAGC GC^T[ACGT]AGC
1529 GCTCAGC
Bpu1102I 18 GCTGAGC GC^T[ACGT]AGC
1529 GCTCAGC
BsaI 988 GGTCTC GGTCTC(1/5)
1080 GGTCTC
BsaWI 731 TCCGGA [AT]^CCGG[AT]
1343 TCCGGA
BseAI 731 TCCGGA T^CCGGA
1343 TCCGGA
BsgI 642 CTGCAC GTGCAG(16/14)
1663 GTGCAG
BspEI 731 TCCGGA T^CCGGA
1343 TCCGGA
BsrBI 24 CCGCTC CCGCTC(-3/-3)
1210 GAGCGG
BstEII 1415 GGTCACC G^GT[ACGT]ACC
1600 GGTTACC
BstPI 1415 GGTCACC G^GT[ACGT]ACC
1600 GGTTACC
CelII 18 GCTGAGC GC^T[ACGT]AGC
1529 GCTCAGC
DpnII 820 GATC ^GATC
828 GATC
EcoNI 450 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1069 CCTGGGGGAGG
EcoO65I 1415 GGTCACC G^GT[ACGT]ACC
1600 GGTTACC
HinfI 202 GATTC G^A[ACGT]TC
1583 GAGTC
MaeI 170 CTAG C^TAG
1523 CTAG
MaeII 900 ACGT A^CGT
1086 ACGT
MboI 820 GATC ^GATC
828 GATC
MroI 731 TCCGGA T^CCGGA
1343 TCCGGA
MseI 926 TTAA T^TAA
965 TTAA
MslI 781 CACCTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1341 CATCCGGATG
NdeII 820 GATC ^GATC
828 GATC
Sau3AI 820 GATC ^GATC
828 GATC
Tru9I 926 TTAA T^TAA
965 TTAA
Tsp509I 692 AATT ^AATT
850 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AfaI AluI AlwNI ApaI
AvaI AvaII BanI BanII BbvI
BcnI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DraII
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MaeIII MboII MnlI
MspA1I MspI MvaI MwoI NciI
NlaIII NlaIV PpuMI PstI RsaI
Sau96I ScrFI SfaNI SfcI StuI
TseI TspRI