Restriction map for
pFN21AE2103
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# NO CUT ENZYMES ( 96 enzymes) #
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Acc65I AclI AflII AgeI AhdI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AviII BbeI BbrPI
BciVI BclI BfrI BglI BlpI
Bpu1102I BsaAI BsaBI BseRI BsiEI
BsiWI BsmBI BspDI BspHI BssSI
BstBI BstXI CelII ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoRI
FbaI FseI FspI HpaI KasI
KpnI KspI MamI MfeI MluI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI XbaI
XhoI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 142 GACGTC GACGT^C
AccIII 128 TCCGGA T^CCGGA
AcsI 2346 GAATTT [GA]^AATT[CT]
AcyI 142 GACGTC G[GA]^CG[CT]C
AlwNI 817 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 504 CCTGAGG CC^T[ACGT]AGG
ApoI 2346 GAATTT [GA]^AATT[CT]
Asp700I 1190 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1237 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 645 CCTAGG C^CTAGG
BanII 2671 GAGCTC G[GA]GC[CT]^C
BbvCI 9 GCTGAGG CCTCAGC(-5/-2)
BcgI 1717 CGAACCGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 2256 AGATCT A^GATCT
BlnI 645 CCTAGG C^CTAGG
BsaHI 142 GACGTC G[GA]^CG[CT]C
BsaI 480 GGTCTC GGTCTC(1/5)
BseAI 128 TCCGGA T^CCGGA
BsmAI 481 GTCTC GTCTC(1/5)
BsmI 2288 GCATTC GAATGC(1/-1)
BspEI 128 TCCGGA T^CCGGA
BspMI 733 GCAGGT ACCTGC(4/8)
BsrBI 2432 CCGCTC CCGCTC(-3/-3)
Bst1107I 2702 GTATAC GTA^TAC
BstAPI 1240 GCAGTCTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 2702 GTATAC GTA^TAC
Bsu36I 504 CCTGAGG CC^T[ACGT]AGG
EarI 511 CTCTTC CTCTTC(1/4)
Eco81I 504 CCTGAGG CC^T[ACGT]AGG
EcoRV 982 GATATC GAT^ATC
EcoT22I 536 ATGCAT ATGCA^T
Hin1I 142 GACGTC G[GA]^CG[CT]C
HincII 1125 GTTGAC GT[CT]^[GA]AC
HindII 1125 GTTGAC GT[CT]^[GA]AC
HindIII 2583 AAGCTT A^AGCTT
Ksp632I 511 CTCTTC CTCTTC(1/4)
MroI 128 TCCGGA T^CCGGA
NcoI 623 CCATGG C^CATGG
NsiI 536 ATGCAT ATGCA^T
PflFI 1237 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 180 GGGTCCC [GA]G^G[AT]CC[CT]
SacI 2671 GAGCTC GAGCT^C
SapI 510 GCTCTTC GCTCTTC(1/4)
SmaI 1467 CCCGGG CCC^GGG
SmlI 2239 CTCAAG C^T[CT][GA]AG
Tth111I 1237 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1467 CCCGGG C^CCGGG
XmaI 1467 CCCGGG C^CCGGG
XmnI 1190 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AflIII 2305 ACATGT A^C[GA][CT]GT
2389 ACATGT
Alw44I 169 GTGCAC G^TGCAC
1566 GTGCAC
ApaLI 169 GTGCAC G^TGCAC
1566 GTGCAC
BalI 915 TGGCCA TGG^CCA
1826 TGGCCA
BfaI 646 CTAG C^TAG
2143 CTAG
BsaWI 128 TCCGGA [AT]^CCGG[AT]
1720 ACCGGA
BsgI 405 CTGCAC GTGCAG(16/14)
967 CTGCAC
BspLU11I 2305 ACATGT A^CATGT
2389 ACATGT
BsrFI 423 GCCGGT [GA]^CCGG[CT]
566 ACCGGC
BstEII 1471 GGTTACC G^GT[ACGT]ACC
1648 GGTTACC
BstPI 1471 GGTTACC G^GT[ACGT]ACC
1648 GGTTACC
Cfr10I 423 GCCGGT [GA]^CCGG[CT]
566 ACCGGC
DraII 180 GGGTCCC [GA]G^G[ACGT]CC[CT]
641 AGGCCCT
DsaI 623 CCATGG C^C[GA][CT]GG
1022 CCACGG
EcoO109I 180 GGGTCCC [GA]G^G[ACGT]CC[CT]
641 AGGCCCT
EcoO65I 1471 GGTTACC G^GT[ACGT]ACC
1648 GGTTACC
MaeI 646 CTAG C^TAG
2143 CTAG
MluNI 915 TGGCCA TGG^CCA
1826 TGGCCA
MscI 915 TGGCCA TGG^CCA
1826 TGGCCA
PflMI 228 CCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
618 CCAGGCCATGG
PvuII 7 CAGCTG CAG^CTG
942 CAGCTG
Van91I 228 CCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
618 CCAGGCCATGG
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# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccI AccII AciI AfaI AluI
AlwI AspHI AvaI AvaII BamHI
BanI BbsI BbvI BcnI BmyI
BpmI BpuAI BsaJI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I Bsp1407I
BsrDI BsrGI BsrI BssHII BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII EaeI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII