Restriction map for
pFN21AE9423
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# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AviII AvrII BanII BbeI
BciVI BfrI BglII BlnI BsaBI
BsaHI BsiEI BsiWI Bsp1407I BspDI
BspLU11I BsrBI BsrDI BsrGI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoRI EcoRV EcoT22I FseI
FspI Hin1I HindIII KasI KpnI
KspI MamI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PmeI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AccI 1111 GTAGAC GT^[AC][GT]AC
AccII 10 CGCG CG^CG
Asp700I 1979 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BcgI 214 GCAACACTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1737 TGATCA T^GATCA
BfaI 1009 CTAG C^TAG
BsaI 955 GAGACC GGTCTC(1/5)
BsmBI 1602 CGTCTC CGTCTC(1/5)
BspHI 1892 TCATGA T^CATGA
BsrFI 1774 GCCGGT [GA]^CCGG[CT]
BstAPI 1493 GCACCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1183 GGTGACC G^GT[ACGT]ACC
BstPI 1183 GGTGACC G^GT[ACGT]ACC
BstUI 10 CGCG CG^CG
BstXI 176 CCACCCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1774 GCCGGT [GA]^CCGG[CT]
DraI 2161 TTTAAA TTT^AAA
DrdI 1114 GACAGGAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1183 GGTGACC G^GT[ACGT]ACC
FbaI 1737 TGATCA T^GATCA
HaeII 918 GGCGCT [GA]GCGC^[CT]
HpaI 2431 GTTAAC GTT^AAC
MaeI 1009 CTAG C^TAG
MfeI 1733 CAATTG C^AATTG
MunI 1733 CAATTG C^AATTG
MvnI 10 CGCG CG^CG
NcoI 182 CCATGG C^CATGG
PflMI 855 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1892 TCATGA T^CATGA
Van91I 855 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1979 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 45 enzymes) #
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AccIII 505 TCCGGA T^CCGGA
2138 TCCGGA
AcsI 241 AAATTC [GA]^AATT[CT]
2061 GAATTT
AfaI 1833 GTAC GT^AC
2298 GTAC
AocI 100 CCTCAGG CC^T[ACGT]AGG
463 CCTGAGG
ApoI 241 AAATTC [GA]^AATT[CT]
2061 GAATTT
AspHI 1405 GTGCTC G[AT]GC[AT]^C
1761 GTGCTC
AspI 766 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
847 GACAAAGTC
BamHI 468 GGATCC G^GATCC
1563 GGATCC
BbrPI 345 CACGTG CAC^GTG
2196 CACGTG
BbsI 1414 GTCTTC GAAGAC(2/6)
2281 GTCTTC
BlpI 1596 GCTGAGC GC^T[ACGT]AGC
2173 GCTCAGC
Bpu1102I 1596 GCTGAGC GC^T[ACGT]AGC
2173 GCTCAGC
BpuAI 1414 GTCTTC GAAGAC(2/6)
2281 GTCTTC
BsaAI 345 CACGTG [CT]AC^GT[GA]
2196 CACGTG
BseAI 505 TCCGGA T^CCGGA
2138 TCCGGA
BsiHKAI 1405 GTGCTC G[AT]GC[AT]^C
1761 GTGCTC
BsmI 496 GAATGC GAATGC(1/-1)
31 GCATTC
BspEI 505 TCCGGA T^CCGGA
2138 TCCGGA
BssSI 111 CTCGTG CACGAG(-5/-1)
1726 CTCGTG
BstYI 468 GGATCC [GA]^GATC[CT]
1563 GGATCC
Bsu36I 100 CCTCAGG CC^T[ACGT]AGG
463 CCTGAGG
CelII 1596 GCTGAGC GC^T[ACGT]AGC
2173 GCTCAGC
CfoI 919 GCGC GCG^C
2045 GCGC
DsaI 182 CCATGG C^C[GA][CT]GG
695 CCGTGG
Eco81I 100 CCTCAGG CC^T[ACGT]AGG
463 CCTGAGG
HhaI 919 GCGC GCG^C
2045 GCGC
HinP1I 919 GCGC G^CGC
2045 GCGC
MflI 468 GGATCC [GA]^GATC[CT]
1563 GGATCC
MroI 505 TCCGGA T^CCGGA
2138 TCCGGA
MseI 2162 TTAA T^TAA
2432 TTAA
PflFI 766 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
847 GACAAAGTC
PmaCI 345 CACGTG CAC^GTG
2196 CACGTG
PmlI 345 CACGTG CAC^GTG
2196 CACGTG
PshAI 361 GACGAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1860 GACGGTGGTC
RsaI 1833 GTAC GT^AC
2298 GTAC
SbfI 1072 CCTGCAGG CCTGCA^GG
2271 CCTGCAGG
SmaI 576 CCCGGG CCC^GGG
2234 CCCGGG
SmlI 1128 CTTGAG C^T[CT][GA]AG
1875 CTCAAG
Sse8387I 1072 CCTGCAGG CCTGCA^GG
2271 CCTGCAGG
StuI 1026 AGGCCT AGG^CCT
1963 AGGCCT
Tru9I 2162 TTAA T^TAA
2432 TTAA
Tth111I 766 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
847 GACAAAGTC
XhoII 468 GGATCC [GA]^GATC[CT]
1563 GGATCC
XmaCI 576 CCCGGG C^CCGGG
2234 CCCGGG
XmaI 576 CCCGGG C^CCGGG
2234 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AflIII AluI AlwI AlwNI
AvaI AvaII BalI BanI BbvCI
BbvI BcnI BglI BmyI BpmI
BsaJI BsaWI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraII
DraIII EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MluNI MnlI
MscI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI