Restriction map for
pFN21AE3953
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AvaII AviII AvrII BamHI BanI
BbeI BbrPI BcgI BcnI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrDI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstZ17I Bsu36I CelII CfoI Cfr10I
Cfr13I ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoRI
EcoRV EcoT14I EcoT22I FauI FseI
FspI HaeII HapII HhaI Hin1I
HinP1I HindIII HpaI HpaII KasI
KpnI KspI MamI MfeI MluI
MroI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NlaIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SapI Sau96I SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI Tth111I TthHB8I XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AciI 360 GCGG CCGC(-3/-1)
AcsI 556 GAATTT [GA]^AATT[CT]
ApoI 556 GAATTT [GA]^AATT[CT]
BbsI 630 GAAGAC GAAGAC(2/6)
BbvCI 655 GCTGAGG CCTCAGC(-5/-2)
BciVI 545 GGATAC GTATCC(6/5)
BglII 282 AGATCT A^GATCT
BpuAI 630 GAAGAC GAAGAC(2/6)
BsaI 362 GGTCTC GGTCTC(1/5)
BsaJI 375 CCGTGG C^C[ACGT][ACGT]GG
BsmFI 44 GTCCC GGGAC(10/14)
BspHI 764 TCATGA T^CATGA
BsrBI 358 GAGCGG CCGCTC(-3/-3)
BstYI 282 AGATCT [GA]^GATC[CT]
DsaI 375 CCGTGG C^C[GA][CT]GG
EcoNI 393 CCTCACGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 633 GACGC GACGC(5/10)
MaeII 23 ACGT A^CGT
MflI 282 AGATCT [GA]^GATC[CT]
MseI 20 TTAA T^TAA
MslI 114 CATCACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 162 CAGCTG C[AC]G^C[GT]G
PflMI 262 CCAGACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 745 CTGCAG CTGCA^G
PvuII 162 CAGCTG CAG^CTG
RcaI 764 TCATGA T^CATGA
SacI 694 GAGCTC GAGCT^C
TfiI 761 GAATC G^A[AT]TC
Tru9I 20 TTAA T^TAA
Tsp509I 557 AATT ^AATT
Van91I 262 CCAGACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 81 TCTAGA T^CTAGA
XhoII 282 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AfaI 25 GTAC GT^AC
526 GTAC
AspHI 370 GAGCAC G[AT]GC[AT]^C
694 GAGCTC
BanII 258 GAGCCC G[GA]GC[CT]^C
694 GAGCTC
BclI 291 TGATCA T^GATCA
561 TGATCA
BfaI 82 CTAG C^TAG
667 CTAG
BpmI 430 CTCCAG CTGGAG(16/14)
55 CTGGAG
BsiHKAI 370 GAGCAC G[AT]GC[AT]^C
694 GAGCTC
BstEII 36 GGTGACC G^GT[ACGT]ACC
379 GGTCACC
BstF5I 576 CATCC GGATG(2/0)
660 GGATG
BstPI 36 GGTGACC G^GT[ACGT]ACC
379 GGTCACC
BstXI 47 CCATCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
224 CCAACATAGTGG
Cac8I 109 GCCTGC GC[ACGT]^[ACGT]GC
347 GCTGGC
EcoO65I 36 GGTGACC G^GT[ACGT]ACC
379 GGTCACC
FbaI 291 TGATCA T^GATCA
561 TGATCA
FokI 576 CATCC GGATG(9/13)
660 GGATG
HincII 63 GTTGAC GT[CT]^[GA]AC
729 GTCAAC
HindII 63 GTTGAC GT[CT]^[GA]AC
729 GTCAAC
MaeI 82 CTAG C^TAG
667 CTAG
RsaI 25 GTAC GT^AC
526 GTAC
SfcI 202 CTACAG C^T[GA][CT]AG
745 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AluI AlwNI BalI BbvI BmyI
BseRI BsiYI BslI BsmAI Bsp1286I
BssKI BstNI DdeI DpnII EaeI
EarI EcoRII Fnu4HI HaeIII HinfI
HphI ItaI Ksp632I MaeIII MboI
MboII MluNI MnlI MscI MvaI
MwoI NdeII NlaIII PleI Sau3AI
ScrFI SfaNI SmlI TseI Tsp45I
TspRI