Restriction map for
pFN21AE3912
##################################
# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BciVI BclI BfrI BlnI BlpI
Bpu1102I BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BsrI BssHII Bst1107I BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI ClaI CpoI DraI
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HpaI
KasI KpnI KspI MamI MluI
MluNI MroI MscI MvnI NarI
NdeI NotI NruI PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SapI
SbfI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AflIII 791 ACACGT A^C[GA][CT]GT
AlwI 732 GATCC GGATC(4/5)
BanI 199 GGCACC G^G[CT][GA]CC
BanII 1198 GAGCTC G[GA]GC[CT]^C
BbsI 360 GAAGAC GAAGAC(2/6)
BcgI 255 CGAGGTCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 295 CCCGG CC^[GC]GG
BfaI 1060 CTAG C^TAG
BglI 685 GCCTTCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 641 AGATCT A^GATCT
BpuAI 360 GAAGAC GAAGAC(2/6)
BsaAI 792 CACGTA [CT]AC^GT[GA]
BsaI 329 GAGACC GGTCTC(1/5)
BseRI 759 GAGGAG GAGGAG(10/8)
BsmAI 329 GAGAC GTCTC(1/5)
BstBI 658 TTCGAA TT^CGAA
BstYI 641 AGATCT [GA]^GATC[CT]
DraIII 211 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 203 CCATGG C^C[GA][CT]GG
EarI 1210 CTCTTC CTCTTC(1/4)
EcoNI 735 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 879 GATATC GAT^ATC
EcoT14I 203 CCATGG C^C[AT][AT]GG
EcoT22I 34 ATGCAT ATGCA^T
HindIII 947 AAGCTT A^AGCTT
Ksp632I 1210 CTCTTC CTCTTC(1/4)
MaeI 1060 CTAG C^TAG
MaeIII 259 GTCAC ^GT[ACGT]AC
MfeI 780 CAATTG C^AATTG
MflI 641 AGATCT [GA]^GATC[CT]
MslI 723 CATGGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 780 CAATTG C^AATTG
NaeI 1160 GCCGGC GCC^GGC
NciI 295 CCCGG CC^[GC]GG
NcoI 203 CCATGG C^CATGG
NgoMIV 1160 GCCGGC G^CCGGC
NheI 1059 GCTAGC G^CTAGC
NsiI 34 ATGCAT ATGCA^T
NspV 658 TTCGAA TT^CGAA
SacI 1198 GAGCTC GAGCT^C
SalI 1186 GTCGAC G^TCGAC
ScaI 113 AGTACT AGT^ACT
SfuI 658 TTCGAA TT^CGAA
SmlI 1097 CTCAAG C^T[CT][GA]AG
StyI 203 CCATGG C^C[AT][AT]GG
Tsp45I 259 GTCAC ^GT[GC]AC
XcmI 898 CCAAGTGTGTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 641 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 556 GTCTAC GT^[AC][GT]AC
1186 GTCGAC
AfaI 114 GTAC GT^AC
795 GTAC
AspHI 3 GAGCAC G[AT]GC[AT]^C
1198 GAGCTC
AvaI 418 CCCGAG C^[CT]CG[GA]G
1201 CTCGGG
AvaII 1004 GGACC G^G[AT]CC
1206 GGACC
BsiHKAI 3 GAGCAC G[AT]GC[AT]^C
1198 GAGCTC
BsmI 868 GAATGC GAATGC(1/-1)
432 GCATTC
BsoBI 418 CCCGAG C^[CT]CG[GA]G
1201 CTCGGG
BsrFI 536 GCCGGT [GA]^CCGG[CT]
1160 GCCGGC
BssSI 165 CACGAG CACGAG(-5/-1)
664 CACGAG
BstAPI 594 GCAAGTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
713 GCAGTATCTGC
Cfr10I 536 GCCGGT [GA]^CCGG[CT]
1160 GCCGGC
HincII 651 GTTGAC GT[CT]^[GA]AC
1186 GTCGAC
HindII 651 GTTGAC GT[CT]^[GA]AC
1186 GTCGAC
HinfI 771 GATTC G^A[ACGT]TC
1234 GAATC
HphI 378 GGTGA GGTGA(8/7)
243 TCACC
MseI 854 TTAA T^TAA
1118 TTAA
NspI 647 GCATGT [GA]CATG^[CT]
1164 GCATGT
PpuMI 1003 GGGACCT [GA]G^G[AT]CC[CT]
1205 GGGACCT
PstI 179 CTGCAG CTGCA^G
396 CTGCAG
RsaI 114 GTAC GT^AC
795 GTAC
StuI 677 AGGCCT AGG^CCT
743 AGGCCT
TfiI 771 GATTC G^A[AT]TC
1234 GAATC
Tru9I 854 TTAA T^TAA
1118 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AluI AlwNI BbvI BmyI
BpmI BsaJI BsgI BsiYI BslI
BsmFI Bsp1286I BspMI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HpaII ItaI MaeII
MboI MboII MnlI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PvuII Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TseI Tsp509I TspRI
TthHB8I