Restriction map for
pFN21AE3617
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# NO CUT ENZYMES ( 108 enzymes) #
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AccI AccIII AclI AflII AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI AspEI AviII AvrII
BbeI BbsI BciVI BclI BfrI
BlnI BlpI Bpu1102I BpuAI BsaI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspEI BspMI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I CelII CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HaeII HindIII
HpaI KasI KspI MfeI MluI
MroI MunI NarI NcoI NheI
NotI NruI NsiI NspV PacI
PaeR7I PmeI PshAI PshBI Psp1406I
PstI PvuI RsrII SacII SalI
SapI SbfI ScaI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI TfiI XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 337 GACGTC GACGT^C
AflIII 369 ACATGT A^C[GA][CT]GT
AlwNI 1111 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 484 CCTGAGG CC^T[ACGT]AGG
Asp700I 268 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 781 GAGCTC G[AT]GC[AT]^C
BalI 225 TGGCCA TGG^CCA
BamHI 480 GGATCC G^GATCC
BanII 781 GAGCTC G[GA]GC[CT]^C
BbrPI 736 CACGTG CAC^GTG
BbvCI 931 GCTGAGG CCTCAGC(-5/-2)
BcgI 975 CGAGATCGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 797 GCCGGATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 41 AGATCT A^GATCT
BsaBI 1024 GATAATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 358 GAGGAG GAGGAG(10/8)
BsiHKAI 781 GAGCTC G[AT]GC[AT]^C
BsmAI 1113 GAGAC GTCTC(1/5)
BspDI 979 ATCGAT AT^CGAT
BspLU11I 369 ACATGT A^CATGT
BsrDI 59 GCAATG GCAATG(2/0)
BstAPI 581 GCATTGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 470 CCATCTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 484 CCTGAGG CC^T[ACGT]AGG
ClaI 979 ATCGAT AT^CGAT
DraIII 938 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 10 CCACGG C^C[GA][CT]GG
EaeI 225 TGGCCA [CT]^GGCC[GA]
Eco81I 484 CCTGAGG CC^T[ACGT]AGG
HincII 538 GTCAAC GT[CT]^[GA]AC
HindII 538 GTCAAC GT[CT]^[GA]AC
MamI 1024 GATAATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 225 TGGCCA TGG^CCA
MscI 225 TGGCCA TGG^CCA
MseI 425 TTAA T^TAA
NaeI 764 GCCGGC GCC^GGC
NdeI 1280 CATATG CA^TATG
NgoMIV 764 GCCGGC G^CCGGC
NspI 369 ACATGT [GA]CATG^[CT]
PflMI 1110 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 736 CACGTG CAC^GTG
PmlI 736 CACGTG CAC^GTG
PvuII 1225 CAGCTG CAG^CTG
SacI 781 GAGCTC GAGCT^C
SexAI 1103 ACCTGGT A^CC[AT]GGT
StuI 1342 AGGCCT AGG^CCT
Tru9I 425 TTAA T^TAA
Van91I 1110 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 268 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Acc65I 1070 GGTACC G^GTACC
1076 GGTACC
AccII 91 CGCG CG^CG
544 CGCG
AcsI 268 GAATTT [GA]^AATT[CT]
1016 AAATTC
AgeI 1067 ACCGGT A^CCGGT
1073 ACCGGT
ApoI 268 GAATTT [GA]^AATT[CT]
1016 AAATTC
Asp718I 1070 GGTACC G^GTACC
1076 GGTACC
BcnI 237 CCGGG CC^[GC]GG
1335 CCGGG
BfaI 613 CTAG C^TAG
853 CTAG
BsaAI 673 TACGTG [CT]AC^GT[GA]
736 CACGTG
BsaWI 1067 ACCGGT [AT]^CCGG[AT]
1073 ACCGGT
BsgI 76 CTGCAC GTGCAG(16/14)
1249 CTGCAC
BspHI 572 TCATGA T^CATGA
725 TCATGA
BsrBI 71 CCGCTC CCGCTC(-3/-3)
747 CCGCTC
BstUI 91 CGCG CG^CG
544 CGCG
EarI 289 GAAGAG CTCTTC(1/4)
912 GAAGAG
EcoNI 351 CCTTAGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1206 CCTCGACAAGG
HgaI 4 GCGTC GACGC(5/10)
989 GCGTC
HphI 678 GGTGA GGTGA(8/7)
1401 TCACC
KpnI 1070 GGTACC GGTAC^C
1076 GGTACC
Ksp632I 289 GAAGAG CTCTTC(1/4)
912 GAAGAG
MaeI 613 CTAG C^TAG
853 CTAG
MspA1I 1225 CAGCTG C[AC]G^C[GT]G
1246 CCGCTG
MvnI 91 CGCG CG^CG
544 CGCG
NciI 237 CCGGG CC^[GC]GG
1335 CCGGG
PinAI 1067 ACCGGT A^CCGGT
1073 ACCGGT
RcaI 572 TCATGA T^CATGA
725 TCATGA
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# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AspI AvaI AvaII BanI BbvI
BmyI BpmI BsaHI BsaJI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII ItaI
MaeII MaeIII MboI MboII MflI
MnlI MslI MspI MvaI MwoI
NdeII NlaIII NlaIV PflFI PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI TaqI TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I XhoII