Restriction map for
pFN21AA1903
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# NO CUT ENZYMES ( 125 enzymes) #
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Acc65I AccI AccII AccIII AflII
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI Asp718I AspI AvaI
AvaII AviII AvrII BbeI BbrPI
BbvCI BcgI BfrI BlnI BlpI
Bpu1102I BsaAI BsaI BseAI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspMI BsrDI BsrFI
BsrGI BssHII Bst1107I BstAPI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
CfoI Cfr10I Cfr13I ClaI CpoI
DraI DraII DraIII DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRI FauI FseI FspI
HaeII HgaI HhaI HinP1I HpaI
KasI KpnI KspI MfeI MluI
MroI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PstI PvuI PvuII RcaI RsrII
SacII SalI SapI Sau96I SbfI
SfiI SfoI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI StuI SwaI
Tth111I Van91I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 1219 GACGTC GACGT^C
AclI 1297 AACGTT AA^CGTT
AcyI 1219 GACGTC G[GA]^CG[CT]C
AhdI 2507 GACCTTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 1749 ATTAAT AT^TAAT
AsnI 1749 ATTAAT AT^TAAT
AspEI 2507 GACCTTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1922 TGGCCA TGG^CCA
BamHI 2788 GGATCC G^GATCC
BanI 2017 GGCACC G^G[CT][GA]CC
BanII 728 GAGCTC G[GA]GC[CT]^C
BciVI 1861 GTATCC GTATCC(6/5)
BclI 2859 TGATCA T^GATCA
BcnI 594 CCCGG CC^[GC]GG
BglI 2834 GCCAAAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1486 AGATCT A^GATCT
BsaBI 2854 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1219 GACGTC G[GA]^CG[CT]C
BsaWI 2168 ACCGGA [AT]^CCGG[AT]
BsgI 640 CTGCAC GTGCAG(16/14)
BsrBI 1258 GAGCGG CCGCTC(-3/-3)
BssSI 1526 CACGAG CACGAG(-5/-1)
BstBI 3378 TTCGAA TT^CGAA
BstXI 1390 CCAGAAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EaeI 1922 TGGCCA [CT]^GGCC[GA]
Eam1105I 2507 GACCTTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2190 CCTTGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1992 ATGCAT ATGCA^T
FbaI 2859 TGATCA T^GATCA
Hin1I 1219 GACGTC G[GA]^CG[CT]C
HincII 2164 GTCAAC GT[CT]^[GA]AC
HindII 2164 GTCAAC GT[CT]^[GA]AC
MamI 2854 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1922 TGGCCA TGG^CCA
MscI 1922 TGGCCA TGG^CCA
MslI 1240 CACAGTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 2012 CCGCTG C[AC]G^C[GT]G
NciI 594 CCCGG CC^[GC]GG
NsiI 1992 ATGCAT ATGCA^T
NspV 3378 TTCGAA TT^CGAA
PshBI 1749 ATTAAT AT^TAAT
Psp1406I 1297 AACGTT AA^CGTT
SacI 728 GAGCTC GAGCT^C
SfuI 3378 TTCGAA TT^CGAA
SmlI 1881 CTCAAG C^T[CT][GA]AG
SphI 3131 GCATGC GCATG^C
XcmI 2387 CCAAAGAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AlwNI 974 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1541 CAGATACTG
BbsI 992 GTCTTC GAAGAC(2/6)
1776 GTCTTC
BpmI 41 CTCCAG CTGGAG(16/14)
980 CTGGAG
BpuAI 992 GTCTTC GAAGAC(2/6)
1776 GTCTTC
DsaI 74 CCATGG C^C[GA][CT]GG
2084 CCATGG
EcoRV 271 GATATC GAT^ATC
1349 GATATC
HaeIII 1923 GGCC GG^CC
2833 GGCC
HapII 595 CCGG C^CGG
2169 CCGG
HindIII 1087 AAGCTT A^AGCTT
1940 AAGCTT
HpaII 595 CCGG C^CGG
2169 CCGG
MspI 595 CCGG C^CGG
2169 CCGG
NcoI 74 CCATGG C^CATGG
2084 CCATGG
PleI 858 GACTC GAGTC(4/5)
1618 GAGTC
ScaI 363 AGTACT AGT^ACT
865 AGTACT
SexAI 2613 ACCTGGT A^CC[AT]GGT
2697 ACCTGGT
TaqI 2736 TCGA T^CGA
3379 TCGA
TthHB8I 2736 TCGA T^CGA
3379 TCGA
XbaI 564 TCTAGA T^CTAGA
1969 TCTAGA
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
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AciI AcsI AfaI AflIII AluI
AlwI ApoI Asp700I AspHI BbvI
BfaI BmyI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspLU11I BsrI BssKI BstF5I
BstNI BstYI Cac8I DdeI DpnII
EarI EcoRII EcoT14I Fnu4HI FokI
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV NspI RsaI Sau3AI
ScrFI SfaNI SfcI SspI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII XmnI