Restriction map for
pFN21AE2071
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII AccI AccII AccIII AclI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI AspEI AspI AvaI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaBI BsaWI BseAI
BsiEI BsiWI BsoBI Bsp1407I BspDI
BspEI BspHI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRV EcoT22I FauI FseI FspI
HincII HindII HindIII HpaI KasI
KspI MamI MfeI MluI MroI
MspA1I MunI MvnI NaeI NarI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacII SalI SbfI SfcI
SfiI SfoI SfuI SgrAI SmaI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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Acc65I 1843 GGTACC G^GTACC
AcyI 37 GGCGTC G[GA]^CG[CT]C
AlwI 1758 GGATC GGATC(4/5)
AlwNI 698 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 1843 GGTACC G^GTACC
BanI 1843 GGTACC G^G[CT][GA]CC
BanII 560 GAGCTC G[GA]GC[CT]^C
BciVI 504 GGATAC GTATCC(6/5)
BclI 354 TGATCA T^GATCA
BsaAI 734 TACGTA [CT]AC^GT[GA]
BsaHI 37 GGCGTC G[GA]^CG[CT]C
BsmI 294 GAATGC GAATGC(1/-1)
BsrBI 2070 GAGCGG CCGCTC(-3/-3)
BsrDI 2081 CATTGC GCAATG(2/0)
BstAPI 1342 GCAGCTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1758 GGATCT [GA]^GATC[CT]
CfoI 1164 GCGC GCG^C
DraII 1809 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoNI 1791 CCTTAGTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1809 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 612 GAATTC G^AATTC
FbaI 354 TGATCA T^GATCA
HaeII 1163 GGCGCT [GA]GCGC^[CT]
HgaI 38 GCGTC GACGC(5/10)
HhaI 1164 GCGC GCG^C
Hin1I 37 GGCGTC G[GA]^CG[CT]C
HinP1I 1164 GCGC G^CGC
KpnI 1843 GGTACC GGTAC^C
MflI 1758 GGATCT [GA]^GATC[CT]
NcoI 1994 CCATGG C^CATGG
NdeI 1026 CATATG CA^TATG
PpuMI 1809 AGGTCCT [GA]G^G[AT]CC[CT]
SacI 560 GAGCTC GAGCT^C
SapI 3 GCTCTTC GCTCTTC(1/4)
ScaI 1282 AGTACT AGT^ACT
SexAI 508 ACCAGGT A^CC[AT]GGT
SfaNI 772 GCATC GCATC(5/9)
SnaBI 734 TACGTA TAC^GTA
XbaI 1639 TCTAGA T^CTAGA
XcmI 24 CCAGCCCCAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1758 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
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AcsI 367 AAATTT [GA]^AATT[CT]
612 GAATTC
AflIII 192 ACATGT A^C[GA][CT]GT
887 ACATGT
ApoI 367 AAATTT [GA]^AATT[CT]
612 GAATTC
BalI 1734 TGGCCA TGG^CCA
1991 TGGCCA
BcnI 1427 CCCGG CC^[GC]GG
2091 CCCGG
BsgI 2127 CTGCAC GTGCAG(16/14)
99 GTGCAG
BsmBI 1464 CGTCTC CGTCTC(1/5)
1865 CGTCTC
BspLU11I 192 ACATGT A^CATGT
887 ACATGT
BspMI 1551 ACCTGC ACCTGC(4/8)
349 GCAGGT
Cac8I 23 GCCAGC GC[ACGT]^[ACGT]GC
1627 GCTTGC
DsaI 1211 CCACGG C^C[GA][CT]GG
1994 CCATGG
EaeI 1734 TGGCCA [CT]^GGCC[GA]
1991 TGGCCA
EcoT14I 1421 CCAAGG C^C[AT][AT]GG
1994 CCATGG
HapII 1428 CCGG C^CGG
2092 CCGG
HpaII 1428 CCGG C^CGG
2092 CCGG
MluNI 1734 TGGCCA TGG^CCA
1991 TGGCCA
MscI 1734 TGGCCA TGG^CCA
1991 TGGCCA
MspI 1428 CCGG C^CGG
2092 CCGG
NciI 1427 CCCGG CC^[GC]GG
2091 CCCGG
NlaIV 318 GGTTCC GG[ACGT]^[ACGT]CC
1843 GGTACC
PflMI 365 CCAAATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1040 CCACACCCTGG
StyI 1421 CCAAGG C^C[AT][AT]GG
1994 CCATGG
Van91I 365 CCAAATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1040 CCACACCCTGG
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AfaI AluI Asp700I AspHI
AvaII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstEII BstF5I
BstNI BstPI Cfr13I DdeI DpnII
EarI EcoO65I EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NspI PleI RsaI
Sau3AI Sau96I ScrFI SmlI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XmnI