Restriction map for
pFN21AB5903
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# NO CUT ENZYMES ( 121 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvrII BamHI BanI BbeI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaWI BseAI
BsiWI BspEI BspHI BspLU11I BsrBI
BssSI Bst1107I BstBI BstEII BstPI
BstYI BstZ17I CelII CpoI Eam1105I
Eco47III EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FseI
HincII HindII HpaI KasI KpnI
KspI MamI MfeI MflI MluI
MroI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PleI PmeI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspI StuI StyI SwaI Tth111I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AcsI 19 AAATTT [GA]^AATT[CT]
AlwI 1381 GATCC GGATC(4/5)
AocI 715 CCTGAGG CC^T[ACGT]AGG
ApoI 19 AAATTT [GA]^AATT[CT]
AspHI 592 GAGCAC G[AT]GC[AT]^C
AviII 653 TGCGCA TGC^GCA
BalI 371 TGGCCA TGG^CCA
BanII 504 GGGCTC G[GA]GC[CT]^C
BbrPI 814 CACGTG CAC^GTG
BbsI 1228 GAAGAC GAAGAC(2/6)
BbvCI 97 CCTCAGC CCTCAGC(-5/-2)
BpmI 1433 CTGGAG CTGGAG(16/14)
BpuAI 1228 GAAGAC GAAGAC(2/6)
BsaAI 814 CACGTG [CT]AC^GT[GA]
BseRI 1436 GAGGAG GAGGAG(10/8)
BsgI 5 CTGCAC GTGCAG(16/14)
BsiEI 150 CGGCCG CG[GA][CT]^CG
BsiHKAI 592 GAGCAC G[AT]GC[AT]^C
BsmBI 415 GAGACG CGTCTC(1/5)
BsmI 1159 GAATGC GAATGC(1/-1)
Bsp1407I 162 TGTACA T^GTACA
BspDI 820 ATCGAT AT^CGAT
BspMI 650 ACCTGC ACCTGC(4/8)
BsrDI 158 GCAATG GCAATG(2/0)
BsrFI 152 GCCGGT [GA]^CCGG[CT]
BsrGI 162 TGTACA T^GTACA
BssHII 567 GCGCGC G^CGCGC
BstAPI 105 GCAGCTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 218 CCACGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 715 CCTGAGG CC^T[ACGT]AGG
Cfr10I 152 GCCGGT [GA]^CCGG[CT]
ClaI 820 ATCGAT AT^CGAT
DraI 1012 TTTAAA TTT^AAA
DraII 92 AGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 595 CACGCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 45 GACCACATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 275 CCGTGG C^C[GA][CT]GG
EagI 150 CGGCCG C^GGCCG
EclXI 150 CGGCCG C^GGCCG
Eco52I 150 CGGCCG C^GGCCG
Eco81I 715 CCTGAGG CC^T[ACGT]AGG
EcoO109I 92 AGGACCC [GA]G^G[ACGT]CC[CT]
FspI 653 TGCGCA TGC^GCA
HaeII 239 GGCGCT [GA]GCGC^[CT]
HindIII 1417 AAGCTT A^AGCTT
MluNI 371 TGGCCA TGG^CCA
MscI 371 TGGCCA TGG^CCA
PmaCI 814 CACGTG CAC^GTG
PmlI 814 CACGTG CAC^GTG
PpuMI 92 AGGACCC [GA]G^G[AT]CC[CT]
PstI 1207 CTGCAG CTGCA^G
SphI 860 GCATGC GCATG^C
SspBI 162 TGTACA T^GTACA
Tsp45I 43 GTGAC ^GT[GC]AC
TspRI 1068 CAGTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 22 enzymes) #
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AcyI 294 GGCGTC G[GA]^CG[CT]C
517 GACGCC
AfaI 163 GTAC GT^AC
767 GTAC
BcnI 639 CCGGG CC^[GC]GG
1330 CCGGG
BglI 75 GCCCCAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
464 GCCCCGAGGGC
BsaHI 294 GGCGTC G[GA]^CG[CT]C
517 GACGCC
BsaI 574 GAGACC GGTCTC(1/5)
669 GGTCTC
EaeI 150 CGGCCG [CT]^GGCC[GA]
371 TGGCCA
HgaI 517 GACGC GACGC(5/10)
295 GCGTC
Hin1I 294 GGCGTC G[GA]^CG[CT]C
517 GACGCC
HinfI 681 GATTC G^A[ACGT]TC
1007 GATTC
MaeII 347 ACGT A^CGT
815 ACGT
MaeIII 43 GTGAC ^GT[ACGT]AC
353 GTAAC
MspA1I 57 CAGCGG C[AC]G^C[GT]G
130 CAGCGG
NciI 639 CCGGG CC^[GC]GG
1330 CCGGG
NspI 536 GCATGT [GA]CATG^[CT]
860 GCATGC
PflMI 299 CCATGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1392 CCAGTCTGTGG
RsaI 163 GTAC GT^AC
767 GTAC
SfaNI 823 GATGC GCATC(5/9)
473 GCATC
SfcI 1207 CTGCAG C^T[GA][CT]AG
1281 CTACAG
SmlI 232 CTCAAG C^T[CT][GA]AG
1248 CTTGAG
TfiI 681 GATTC G^A[AT]TC
1007 GATTC
Van91I 299 CCATGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1392 CCAGTCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccII AciI AluI Asp700I AvaI
AvaII BbvI BfaI BmyI BsaJI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII EarI EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI Ksp632I MaeI MboI
MboII MnlI MseI MslI MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI TaqI
Tru9I TseI Tsp509I TthHB8I XmnI