Restriction map for
pFN21AE1953
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# NO CUT ENZYMES ( 143 enzymes) #
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AatII Acc65I AccII AciI AclI
AcyI AflII AgeI AhdI AocI
Aor51HI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BanI BbeI BbrPI BcgI BciVI
BclI BcnI BfaI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaI BseRI BsiEI BsiWI BsmBI
BsmFI BsmI BsoBI BspDI BspLU11I
BspMI BsrBI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HgaI Hin1I HindIII HpaI KasI
KpnI KspI MaeI MfeI MluI
MunI MvnI MwoI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I StuI SwaI
Tsp45I Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccIII 387 TCCGGA T^CCGGA
AflIII 372 ACGTGT A^C[GA][CT]GT
Alw44I 1144 GTGCAC G^TGCAC
AlwNI 1108 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 928 GGGCCC GGGCC^C
ApaLI 1144 GTGCAC G^TGCAC
AspHI 1144 GTGCAC G[AT]GC[AT]^C
BalI 666 TGGCCA TGG^CCA
BamHI 420 GGATCC G^GATCC
BanII 928 GGGCCC G[GA]GC[CT]^C
BbsI 1051 GTCTTC GAAGAC(2/6)
BbvCI 701 CCTCAGC CCTCAGC(-5/-2)
BglII 500 AGATCT A^GATCT
BpuAI 1051 GTCTTC GAAGAC(2/6)
BsaBI 979 GATCTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 387 TCCGGA [AT]^CCGG[AT]
BseAI 387 TCCGGA T^CCGGA
BsgI 960 CTGCAC GTGCAG(16/14)
BsiHKAI 1144 GTGCAC G[AT]GC[AT]^C
BsmAI 205 GAGAC GTCTC(1/5)
Bsp1407I 1010 TGTACA T^GTACA
BspEI 387 TCCGGA T^CCGGA
BsrDI 350 CATTGC GCAATG(2/0)
BsrGI 1010 TGTACA T^GTACA
BstXI 94 CCAGTAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 357 GCGC GCG^C
DraI 268 TTTAAA TTT^AAA
DraII 928 GGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 666 TGGCCA [CT]^GGCC[GA]
EarI 1096 GAAGAG CTCTTC(1/4)
EcoO109I 928 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 1080 CCAAGG C^C[AT][AT]GG
HaeII 356 AGCGCC [GA]GCGC^[CT]
HapII 388 CCGG C^CGG
HhaI 357 GCGC GCG^C
HinP1I 357 GCGC G^CGC
HincII 280 GTCAAC GT[CT]^[GA]AC
HindII 280 GTCAAC GT[CT]^[GA]AC
HpaII 388 CCGG C^CGG
Ksp632I 1096 GAAGAG CTCTTC(1/4)
MaeIII 586 GTAAC ^GT[ACGT]AC
MamI 979 GATCTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 666 TGGCCA TGG^CCA
MroI 387 TCCGGA T^CCGGA
MscI 666 TGGCCA TGG^CCA
MslI 285 CATTCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 388 CCGG C^CGG
NdeI 527 CATATG CA^TATG
NspI 1333 GCATGC [GA]CATG^[CT]
SmlI 555 CTTGAG C^T[CT][GA]AG
SphI 1333 GCATGC GCATG^C
SspBI 1010 TGTACA T^GTACA
StyI 1080 CCAAGG C^C[AT][AT]GG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AccI 404 GTAGAC GT^[AC][GT]AC
855 GTCTAC
AcsI 744 AAATTT [GA]^AATT[CT]
817 GAATTT
AfaI 738 GTAC GT^AC
1011 GTAC
ApoI 744 AAATTT [GA]^AATT[CT]
817 GAATTT
Asp700I 1047 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1068 GAAAGACTTC
AvaII 1035 GGACC G^G[AT]CC
1084 GGACC
BmyI 928 GGGCCC G[AGT]GC[ACT]^C
1144 GTGCAC
Bsp1286I 928 GGGCCC G[AGT]GC[ACT]^C
1144 GTGCAC
Cac8I 1164 GCCAGC GC[ACGT]^[ACGT]GC
1333 GCATGC
MspA1I 643 CAGCTG C[AC]G^C[GT]G
1108 CAGCTG
PvuII 643 CAGCTG CAG^CTG
1108 CAGCTG
RsaI 738 GTAC GT^AC
1011 GTAC
SexAI 135 ACCTGGT A^CC[AT]GGT
1349 ACCTGGT
SfcI 857 CTACAG C^T[GA][CT]AG
1175 CTATAG
SspI 432 AATATT AAT^ATT
724 AATATT
TaqI 633 TCGA T^CGA
795 TCGA
TthHB8I 633 TCGA T^CGA
795 TCGA
XmnI 1047 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1068 GAAAGACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AluI AlwI BbvI BpmI BsaJI
BsiYI BslI BspHI BsrI BssKI
BstF5I BstNI BstYI Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeII MboI
MboII MflI MnlI MseI MvaI
NdeII NlaIII NlaIV RcaI Sau3AI
Sau96I ScrFI SfaNI TfiI Tru9I
TseI Tsp509I TspRI XhoII