Restriction map for
pFN21AB5773
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# NO CUT ENZYMES ( 98 enzymes) #
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AccII AclI AcsI AgeI AhdI
ApoI AscI AseI AsnI AspEI
AspI AvrII BbeI BbrPI BciVI
BclI BglII BlnI BsaBI BsiWI
Bsp1407I BspDI BspHI BsrDI BsrFI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Cfr10I ClaI
CpoI DraI DrdI Eam1105I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HincII HindII HindIII HpaI KasI
KspI MamI MfeI MluI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI ScaI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI StuI SwaI Tsp509I Tth111I
XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 817 GACGTC GACGT^C
Acc65I 1379 GGTACC G^GTACC
AccI 74 GTCTAC GT^[AC][GT]AC
AflII 1279 CTTAAG C^TTAAG
Alw44I 1423 GTGCAC G^TGCAC
Aor51HI 1107 AGCGCT AGC^GCT
ApaLI 1423 GTGCAC G^TGCAC
Asp700I 1552 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1379 GGTACC G^GTACC
BamHI 1826 GGATCC G^GATCC
BanI 1379 GGTACC G^G[CT][GA]CC
BbsI 1971 GAAGAC GAAGAC(2/6)
BbvCI 2289 CCTCAGC CCTCAGC(-5/-2)
BcgI 327 GCAGCAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1562 CTAG C^TAG
BfrI 1279 CTTAAG C^TTAAG
BglI 26 GCCCCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1971 GAAGAC GAAGAC(2/6)
BsaAI 1471 TACGTG [CT]AC^GT[GA]
BsaI 2254 GAGACC GGTCTC(1/5)
BsiEI 2202 CGGCCG CG[GA][CT]^CG
BsmI 1565 GCATTC GAATGC(1/-1)
BspLU11I 149 ACATGT A^CATGT
BsrBI 1045 GAGCGG CCGCTC(-3/-3)
BssSI 646 CACGAG CACGAG(-5/-1)
BstXI 959 CCACCGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 824 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2202 CGGCCG C^GGCCG
EclXI 2202 CGGCCG C^GGCCG
Eco47III 1107 AGCGCT AGC^GCT
Eco52I 2202 CGGCCG C^GGCCG
HaeII 1107 AGCGCT [GA]GCGC^[CT]
KpnI 1379 GGTACC GGTAC^C
MaeI 1562 CTAG C^TAG
NspI 149 ACATGT [GA]CATG^[CT]
PflMI 1518 CCAGTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 414 GAAGAGC GCTCTTC(1/4)
SexAI 1679 ACCTGGT A^CC[AT]GGT
SmaI 1040 CCCGGG CCC^GGG
TfiI 1298 GAATC G^A[AT]TC
Van91I 1518 CCAGTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1040 CCCGGG C^CCGGG
XmaI 1040 CCCGGG C^CCGGG
XmnI 1552 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccIII 1013 TCCGGA T^CCGGA
1344 TCCGGA
AcyI 817 GACGTC G[GA]^CG[CT]C
1733 GACGCC
AflIII 149 ACATGT A^C[GA][CT]GT
2225 ACGTGT
AviII 518 TGCGCA TGC^GCA
576 TGCGCA
BalI 725 TGGCCA TGG^CCA
956 TGGCCA
BlpI 303 GCTGAGC GC^T[ACGT]AGC
1411 GCTGAGC
Bpu1102I 303 GCTGAGC GC^T[ACGT]AGC
1411 GCTGAGC
BsaHI 817 GACGTC G[GA]^CG[CT]C
1733 GACGCC
BsaWI 1013 TCCGGA [AT]^CCGG[AT]
1344 TCCGGA
BseAI 1013 TCCGGA T^CCGGA
1344 TCCGGA
BsmBI 1861 CGTCTC CGTCTC(1/5)
1780 GAGACG
BspEI 1013 TCCGGA T^CCGGA
1344 TCCGGA
BspMI 641 ACCTGC ACCTGC(4/8)
1101 GCAGGT
CelII 303 GCTGAGC GC^T[ACGT]AGC
1411 GCTGAGC
DsaI 36 CCACGG C^C[GA][CT]GG
1383 CCACGG
EcoNI 642 CCTGCACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
705 CCTGGTGCAGG
FspI 518 TGCGCA TGC^GCA
576 TGCGCA
Hin1I 817 GACGTC G[GA]^CG[CT]C
1733 GACGCC
MluNI 725 TGGCCA TGG^CCA
956 TGGCCA
MroI 1013 TCCGGA T^CCGGA
1344 TCCGGA
MscI 725 TGGCCA TGG^CCA
956 TGGCCA
MseI 1280 TTAA T^TAA
2195 TTAA
PleI 1974 GACTC GAGTC(4/5)
844 GAGTC
Tru9I 1280 TTAA T^TAA
2195 TTAA
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# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
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AciI AfaI AluI AlwI AlwNI
AocI ApaI AspHI AvaI AvaII
BanII BbvI BcnI BmyI BpmI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstAPI BstF5I BstNI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I SbfI
ScrFI SfaNI SfcI SmlI Sse8387I
StyI TaqI TseI Tsp45I TspRI
TthHB8I XhoII