Restriction map for
pFN21AE2034
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# NO CUT ENZYMES ( 91 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI AhdI AlwI AocI Aor51HI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BamHI BbrPI BcgI
BciVI BclI BfrI BlnI BpmI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BsrGI Bst1107I BstBI BstZ17I
Bsu36I ClaI CpoI DraI Eam1105I
Eco47III Eco81I EcoRI EcoRV FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI MluI MroI MseI
NdeI NheI NotI NruI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SalI SbfI SfiI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tru9I Van91I XbaI
XhoI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 490 GACGTC GACGT^C
AcsI 532 AAATTC [GA]^AATT[CT]
AflIII 1409 ACATGT A^C[GA][CT]GT
Alw44I 988 GTGCAC G^TGCAC
ApaLI 988 GTGCAC G^TGCAC
ApoI 532 AAATTC [GA]^AATT[CT]
Asp700I 126 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 1522 GGCGCC GGCGC^C
BfaI 7 CTAG C^TAG
BglII 683 AGATCT A^GATCT
BsaAI 1777 TACGTG [CT]AC^GT[GA]
BsaBI 1812 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1484 GAGACG CGTCTC(1/5)
BspHI 1070 TCATGA T^CATGA
BspLU11I 1409 ACATGT A^CATGT
BsrDI 1719 CATTGC GCAATG(2/0)
BssHII 72 GCGCGC G^CGCGC
BssSI 1182 CACGAG CACGAG(-5/-1)
BstAPI 1011 GCACACCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 402 GGTCACC G^GT[ACGT]ACC
BstPI 402 GGTCACC G^GT[ACGT]ACC
BstXI 893 CCACCTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 683 AGATCT [GA]^GATC[CT]
DraIII 825 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 394 GACTTCGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 411 CCTGACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 402 GGTCACC G^GT[ACGT]ACC
EcoT22I 1123 ATGCAT ATGCA^T
KasI 1522 GGCGCC G^GCGCC
KspI 25 CCGCGG CCGC^GG
MaeI 7 CTAG C^TAG
MamI 1812 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1098 CAATTG C^AATTG
MflI 683 AGATCT [GA]^GATC[CT]
MunI 1098 CAATTG C^AATTG
NarI 1522 GGCGCC GG^CGCC
NcoI 1784 CCATGG C^CATGG
NsiI 1123 ATGCAT ATGCA^T
PstI 480 CTGCAG CTGCA^G
RcaI 1070 TCATGA T^CATGA
SacII 25 CCGCGG CCGC^GG
SapI 729 GCTCTTC GCTCTTC(1/4)
ScaI 1469 AGTACT AGT^ACT
SexAI 569 ACCTGGT A^CC[AT]GGT
SfcI 480 CTGCAG C^T[GA][CT]AG
SfoI 1522 GGCGCC GGC^GCC
XhoII 683 AGATCT [GA]^GATC[CT]
XmnI 126 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwNI 1195 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1297 CAGCCCCTG
ApaI 12 GGGCCC GGGCC^C
98 GGGCCC
AspI 178 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
364 GACATCGTC
BbvCI 1291 CCTCAGC CCTCAGC(-5/-2)
1395 CCTCAGC
BglI 1495 GCCCCCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1857 GCCGCCCTGGC
BsaI 91 GAGACC GGTCTC(1/5)
1454 GGTCTC
BsaWI 136 TCCGGT [AT]^CCGG[AT]
1445 TCCGGT
EagI 358 CGGCCG C^GGCCG
1078 CGGCCG
EclXI 358 CGGCCG C^GGCCG
1078 CGGCCG
Eco52I 358 CGGCCG C^GGCCG
1078 CGGCCG
EcoT14I 416 CCAAGG C^C[AT][AT]GG
1784 CCATGG
HaeII 21 AGCGCC [GA]GCGC^[CT]
1522 GGCGCC
HinfI 453 GACTC G^A[ACGT]TC
1117 GAGTC
MslI 219 CATTGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
834 CATCTCCGTG
PflFI 178 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
364 GACATCGTC
PleI 453 GACTC GAGTC(4/5)
1117 GAGTC
PpuMI 299 GGGACCC [GA]G^G[AT]CC[CT]
1390 AGGTCCC
PvuII 305 CAGCTG CAG^CTG
598 CAGCTG
SmaI 95 CCCGGG CCC^GGG
1694 CCCGGG
StyI 416 CCAAGG C^C[AT][AT]GG
1784 CCATGG
Tsp509I 533 AATT ^AATT
1099 AATT
Tth111I 178 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
364 GACATCGTC
XmaCI 95 CCCGGG C^CCGGG
1694 CCCGGG
XmaI 95 CCCGGG C^CCGGG
1694 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BcnI BlpI
BmyI Bpu1102I BpuAI BsaHI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MluNI MnlI
MscI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI TaqI
TseI Tsp45I TspRI TthHB8I XcmI