Restriction map for
pFN21AB8543
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# NO CUT ENZYMES ( 83 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AviII AvrII BamHI BbeI
BbrPI BciVI BclI BfrI BlnI
BsaAI BsaBI BsaHI BseAI BsiWI
BsmI BspDI BspEI BsrBI BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI DrdI Eco47III EcoO65I EcoRV
FbaI FseI FspI Hin1I HincII
HindII HindIII HpaI KasI KpnI
MamI MroI NarI NdeI NotI
NruI NspV PacI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
SalI ScaI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI SspI
SwaI XbaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AhdI 350 GACCGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 117 CCTGAGG CC^T[ACGT]AGG
AspEI 350 GACCGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 494 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 1773 CGAGGTGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 2553 AGATCT A^GATCT
BsaWI 2903 TCCGGT [AT]^CCGG[AT]
Bsp1407I 1226 TGTACA T^GTACA
BspLU11I 2324 ACATGT A^CATGT
BsrDI 929 CATTGC GCAATG(2/0)
BsrGI 1226 TGTACA T^GTACA
BssHII 233 GCGCGC G^CGCGC
BstXI 2073 CCAGGCCGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 117 CCTGAGG CC^T[ACGT]AGG
DraI 1147 TTTAAA TTT^AAA
DraIII 2274 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 424 CGGCCG C^GGCCG
Eam1105I 350 GACCGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 424 CGGCCG C^GGCCG
Eco52I 424 CGGCCG C^GGCCG
Eco81I 117 CCTGAGG CC^T[ACGT]AGG
EcoRI 875 GAATTC G^AATTC
EcoT22I 2391 ATGCAT ATGCA^T
MaeII 2699 ACGT A^CGT
MfeI 1082 CAATTG C^AATTG
MluI 2640 ACGCGT A^CGCGT
MunI 1082 CAATTG C^AATTG
NheI 1866 GCTAGC G^CTAGC
NsiI 2391 ATGCAT ATGCA^T
NspI 2324 ACATGT [GA]CATG^[CT]
PaeR7I 1771 CTCGAG C^TCGAG
PflFI 494 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 514 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 193 GAAGAGC GCTCTTC(1/4)
SbfI 124 CCTGCAGG CCTGCA^GG
SexAI 2814 ACCAGGT A^CC[AT]GGT
SmlI 1771 CTCGAG C^T[CT][GA]AG
Sse8387I 124 CCTGCAGG CCTGCA^GG
SspBI 1226 TGTACA T^GTACA
Tth111I 494 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1771 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AcsI 875 GAATTC [GA]^AATT[CT]
2665 AAATTC
AflIII 2324 ACATGT A^C[GA][CT]GT
2640 ACGCGT
ApoI 875 GAATTC [GA]^AATT[CT]
2665 AAATTC
BalI 1709 TGGCCA TGG^CCA
2258 TGGCCA
BbvCI 2295 CCTCAGC CCTCAGC(-5/-2)
2026 GCTGAGG
BlpI 1803 GCTCAGC GC^T[ACGT]AGC
2822 GCTCAGC
Bpu1102I 1803 GCTCAGC GC^T[ACGT]AGC
2822 GCTCAGC
BsaI 385 GAGACC GGTCTC(1/5)
2155 GAGACC
BsiEI 38 CGACCG CG[GA][CT]^CG
424 CGGCCG
BsmBI 418 GAGACG CGTCTC(1/5)
2205 GAGACG
BspHI 1764 TCATGA T^CATGA
2790 TCATGA
BstAPI 435 GCAAGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2892 GCAGGAGCTGC
CelII 1803 GCTCAGC GC^T[ACGT]AGC
2822 GCTCAGC
CpoI 348 CGGACCG CG^G[AT]CCG
402 CGGACCG
EarI 649 CTCTTC CTCTTC(1/4)
193 GAAGAG
Ksp632I 649 CTCTTC CTCTTC(1/4)
193 GAAGAG
KspI 406 CCGCGG CCGC^GG
480 CCGCGG
MluNI 1709 TGGCCA TGG^CCA
2258 TGGCCA
MscI 1709 TGGCCA TGG^CCA
2258 TGGCCA
RcaI 1764 TCATGA T^CATGA
2790 TCATGA
RsrII 348 CGGACCG CG^G[AT]CCG
402 CGGACCG
SacII 406 CCGCGG CCGC^GG
480 CCGCGG
XcmI 1190 CCAGTGCCTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2226 CCATGGGCCCCCTGG
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# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmaCI
XmaI