Restriction map for
pFN21AE1594
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# NO CUT ENZYMES ( 152 enzymes) #
##################################
Acc65I AccII AccIII AciI AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AvaII AviII AvrII
BamHI BanI BbeI BbrPI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BsoBI BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstF5I BstPI BstUI BstZ17I Bsu36I
CelII CfoI Cfr10I Cfr13I ClaI
CpoI DraI DraII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FokI FseI FspI
HaeII HgaI HhaI HinP1I HpaI
KasI KpnI KspI MamI MluI
MroI MslI MvnI NaeI NarI
NdeI NgoMIV NheI NlaIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SapI Sau96I
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tth111I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AatII 776 GACGTC GACGT^C
AccI 773 GTCGAC GT^[AC][GT]AC
AcyI 776 GACGTC G[GA]^CG[CT]C
BalI 138 TGGCCA TGG^CCA
BbsI 251 GAAGAC GAAGAC(2/6)
BbvCI 735 GCTGAGG CCTCAGC(-5/-2)
BcnI 679 CCCGG CC^[GC]GG
BpmI 805 CTGGAG CTGGAG(16/14)
BpuAI 251 GAAGAC GAAGAC(2/6)
BsaHI 776 GACGTC G[GA]^CG[CT]C
BseRI 503 CTCCTC GAGGAG(10/8)
BsgI 124 GTGCAG GTGCAG(16/14)
BsmAI 184 GAGAC GTCTC(1/5)
Bsp1407I 525 TGTACA T^GTACA
BsrDI 408 CATTGC GCAATG(2/0)
BsrGI 525 TGTACA T^GTACA
BstAPI 172 GCAGCAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 72 CCAGCATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 509 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 138 TGGCCA [CT]^GGCC[GA]
EarI 319 GAAGAG CTCTTC(1/4)
HaeIII 139 GGCC GG^CC
HapII 680 CCGG C^CGG
Hin1I 776 GACGTC G[GA]^CG[CT]C
HincII 773 GTCGAC GT[CT]^[GA]AC
HindII 773 GTCGAC GT[CT]^[GA]AC
HindIII 844 AAGCTT A^AGCTT
HpaII 680 CCGG C^CGG
Ksp632I 319 GAAGAG CTCTTC(1/4)
MaeIII 608 GTGAC ^GT[ACGT]AC
MluNI 138 TGGCCA TGG^CCA
MscI 138 TGGCCA TGG^CCA
MspI 680 CCGG C^CGG
NciI 679 CCCGG CC^[GC]GG
NspI 611 ACATGC [GA]CATG^[CT]
PflMI 699 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 129 GACTC GAGTC(4/5)
SalI 773 GTCGAC G^TCGAC
SexAI 510 ACCAGGT A^CC[AT]GGT
SfaNI 673 GCATC GCATC(5/9)
SspBI 525 TGTACA T^GTACA
Tsp45I 608 GTGAC ^GT[GC]AC
Van91I 699 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcsI 144 AAATTC [GA]^AATT[CT]
492 AAATTC
AlwI 627 GATCC GGATC(4/5)
451 GGATC
ApoI 144 AAATTC [GA]^AATT[CT]
492 AAATTC
AspHI 500 GAGCTC G[AT]GC[AT]^C
632 GAGCTC
BanII 500 GAGCTC G[GA]GC[CT]^C
632 GAGCTC
BmyI 500 GAGCTC G[AGT]GC[ACT]^C
632 GAGCTC
BsiHKAI 500 GAGCTC G[AT]GC[AT]^C
632 GAGCTC
Bsp1286I 500 GAGCTC G[AGT]GC[ACT]^C
632 GAGCTC
BsrI 136 ACTGG ACTGG(1/-1)
604 ACTGG
BstYI 451 GGATCT [GA]^GATC[CT]
626 AGATCC
Cac8I 474 GCAGGC GC[ACGT]^[ACGT]GC
705 GCTGGC
DsaI 53 CCATGG C^C[GA][CT]GG
362 CCATGG
EcoNI 257 CCTCTTATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
505 CCTCCACCAGG
HphI 607 GGTGA GGTGA(8/7)
663 GGTGA
MaeII 231 ACGT A^CGT
777 ACGT
MfeI 176 CAATTG C^AATTG
521 CAATTG
MflI 451 GGATCT [GA]^GATC[CT]
626 AGATCC
MseI 29 TTAA T^TAA
481 TTAA
MspA1I 545 CAGCTG C[AC]G^C[GT]G
703 CAGCTG
MunI 176 CAATTG C^AATTG
521 CAATTG
NcoI 53 CCATGG C^CATGG
362 CCATGG
PstI 197 CTGCAG CTGCA^G
472 CTGCAG
PvuII 545 CAGCTG CAG^CTG
703 CAGCTG
SacI 500 GAGCTC GAGCT^C
632 GAGCTC
TaqI 774 TCGA T^CGA
780 TCGA
Tru9I 29 TTAA T^TAA
481 TTAA
TspRI 603 CACTG CA[GC]TG[ACGT][ACGT]^
821 CAGTG
TthHB8I 774 TCGA T^CGA
780 TCGA
XhoII 451 GGATCT [GA]^GATC[CT]
626 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AfaI AluI AlwNI BbvI BfaI
BsaJI BsiYI BslI BsmFI BssKI
BstNI DdeI DpnII EcoRII EcoT14I
Fnu4HI HinfI ItaI MaeI MboI
MboII MnlI MvaI MwoI NdeII
NlaIII RsaI Sau3AI ScrFI SfcI
StyI TfiI TseI Tsp509I