Restriction map for
pFN21AB8130
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# NO CUT ENZYMES ( 100 enzymes) #
##################################
Acc65I AccIII AclI AcsI AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AvrII BamHI BbeI
BbrPI BbvCI BfaI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsiWI BsmBI BspDI BspEI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I CelII ClaI
CpoI DraI DraIII Eam1105I Eco47III
EcoO65I EcoRI EcoRV EcoT22I FseI
HindIII HpaI KasI KpnI KspI
MaeI MamI MfeI MluI MroI
MunI NarI NdeI NheI NruI
NsiI NspV PacI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I RsrII SacI SacII SalI
SbfI ScaI SfiI SfoI SfuI
SnaBI SpeI SphI Sse8387I SspI
SwaI TfiI Van91I XbaI XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AatII 435 GACGTC GACGT^C
AccI 715 GTCTAC GT^[AC][GT]AC
AccII 1174 CGCG CG^CG
AcyI 435 GACGTC G[GA]^CG[CT]C
AflII 415 CTTAAG C^TTAAG
AocI 802 CCTGAGG CC^T[ACGT]AGG
AspHI 1159 GTGCTC G[AT]GC[AT]^C
AspI 1048 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 581 TGCGCA TGC^GCA
BbsI 60 GAAGAC GAAGAC(2/6)
BcgI 704 GCAACCTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 385 GGATAC GTATCC(6/5)
BclI 671 TGATCA T^GATCA
BfrI 415 CTTAAG C^TTAAG
BglI 869 GCCACAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 60 GAAGAC GAAGAC(2/6)
BsaHI 435 GACGTC G[GA]^CG[CT]C
BsgI 1246 CTGCAC GTGCAG(16/14)
BsiHKAI 1159 GTGCTC G[AT]GC[AT]^C
BsmI 780 GCATTC GAATGC(1/-1)
BspHI 1058 TCATGA T^CATGA
BspLU11I 563 ACATGT A^CATGT
BsrBI 819 CCGCTC CCGCTC(-3/-3)
BsrDI 1278 CATTGC GCAATG(2/0)
BssSI 226 CACGAG CACGAG(-5/-1)
BstUI 1174 CGCG CG^CG
Bsu36I 802 CCTGAGG CC^T[ACGT]AGG
DrdI 558 GACCTACATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 802 CCTGAGG CC^T[ACGT]AGG
EcoNI 660 CCTTACGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 464 CCATGG C^C[AT][AT]GG
FbaI 671 TGATCA T^GATCA
FspI 581 TGCGCA TGC^GCA
HaeII 1193 AGCGCC [GA]GCGC^[CT]
HgaI 1052 GCGTC GACGC(5/10)
Hin1I 435 GACGTC G[GA]^CG[CT]C
HincII 541 GTCAAC GT[CT]^[GA]AC
HindII 541 GTCAAC GT[CT]^[GA]AC
HinfI 63 GACTC G^A[ACGT]TC
MseI 416 TTAA T^TAA
MslI 1209 CATCAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 1174 CGCG CG^CG
NcoI 464 CCATGG C^CATGG
NotI 875 GCGGCCGC GC^GGCCGC
PaeR7I 1131 CTCGAG C^TCGAG
PflFI 1048 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 63 GACTC GAGTC(4/5)
PstI 442 CTGCAG CTGCA^G
PvuI 1095 CGATCG CGAT^CG
RcaI 1058 TCATGA T^CATGA
SapI 796 GAAGAGC GCTCTTC(1/4)
SexAI 956 ACCAGGT A^CC[AT]GGT
SgrAI 1195 CGCCGGCG C[GA]^CCGG[CT]G
StuI 1127 AGGCCT AGG^CCT
StyI 464 CCATGG C^C[AT][AT]GG
Tru9I 416 TTAA T^TAA
Tsp509I 347 AATT ^AATT
Tth111I 1048 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 464 CCATGGATCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1131 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 203 ACGTGT A^C[GA][CT]GT
563 ACATGT
AlwNI 546 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
677 CAGCATCTG
BanII 486 GAGCCC G[GA]GC[CT]^C
732 GGGCTC
BsaI 556 GAGACC GGTCTC(1/5)
949 GAGACC
BseRI 843 CTCCTC GAGGAG(10/8)
265 GAGGAG
BsmAI 556 GAGAC GTCTC(1/5)
949 GAGAC
Bsp1407I 206 TGTACA T^GTACA
1304 TGTACA
BspMI 578 ACCTGC ACCTGC(4/8)
444 GCAGGT
BsrFI 642 GCCGGC [GA]^CCGG[CT]
1196 GCCGGC
BsrGI 206 TGTACA T^GTACA
1304 TGTACA
BsrI 451 CCAGT ACTGG(1/-1)
812 CCAGT
Cfr10I 642 GCCGGC [GA]^CCGG[CT]
1196 GCCGGC
EagI 135 CGGCCG C^GGCCG
876 CGGCCG
EclXI 135 CGGCCG C^GGCCG
876 CGGCCG
Eco52I 135 CGGCCG C^GGCCG
876 CGGCCG
NaeI 642 GCCGGC GCC^GGC
1196 GCCGGC
NgoMIV 642 GCCGGC G^CCGGC
1196 GCCGGC
PvuII 68 CAGCTG CAG^CTG
761 CAGCTG
SfaNI 584 GCATC GCATC(5/9)
679 GCATC
SfcI 442 CTGCAG C^T[GA][CT]AG
717 CTACAG
SmaI 893 CCCGGG CCC^GGG
1151 CCCGGG
SspBI 206 TGTACA T^GTACA
1304 TGTACA
XmaCI 893 CCCGGG C^CCGGG
1151 CCCGGG
XmaI 893 CCCGGG C^CCGGG
1151 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaI
AvaII BalI BanI BbvI BcnI
BmyI BpmI BsaJI BsiEI BsiYI
BslI BsmFI BsoBI Bsp1286I BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PpuMI RsaI Sau3AI
Sau96I ScrFI SmlI TaqI TseI
Tsp45I TspRI TthHB8I XhoII