Restriction map for
pFN21AB6914
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# NO CUT ENZYMES ( 188 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspHI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanII BbeI BbrPI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstNI BstPI BstUI BstXI BstYI
BstZ17I Bsu36I Cac8I CelII Cfr10I
Cfr13I ClaI CpoI DraI DraII
DraIII DrdI DsaI EaeI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRII EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI HphI KasI KpnI KspI
MaeII MaeIII MamI MfeI MflI
MluI MluNI MroI MscI MspA1I
MunI MvaI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
Sau96I SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI TaqI Tsp45I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AhdI 248 GACTTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 469 GGATC GGATC(4/5)
Asp700I 494 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 248 GACTTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 46 GGCACC G^G[CT][GA]CC
BbsI 401 GTCTTC GAAGAC(2/6)
BbvCI 27 GCTGAGG CCTCAGC(-5/-2)
BcnI 43 CCGGG CC^[GC]GG
BmyI 45 GGGCAC G[AGT]GC[ACT]^C
BpuAI 401 GTCTTC GAAGAC(2/6)
BsgI 329 GTGCAG GTGCAG(16/14)
BsiYI 178 CCACCTGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 178 CCACCTGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 16 GGGAC GGGAC(10/14)
BsmI 271 GAATGC GAATGC(1/-1)
Bsp1286I 45 GGGCAC G[AGT]GC[ACT]^C
BssKI 43 CCGGG ^CC[ACGT]GG
BstF5I 277 CATCC GGATG(2/0)
CfoI 55 GCGC GCG^C
DpnII 470 GATC ^GATC
Eam1105I 248 GACTTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 634 GAAGAG CTCTTC(1/4)
EcoRI 648 GAATTC G^AATTC
FokI 277 CATCC GGATG(9/13)
HapII 43 CCGG C^CGG
HhaI 55 GCGC GCG^C
HinP1I 55 GCGC G^CGC
HpaII 43 CCGG C^CGG
Ksp632I 634 GAAGAG CTCTTC(1/4)
MboI 470 GATC ^GATC
MseI 220 TTAA T^TAA
MspI 43 CCGG C^CGG
NciI 43 CCGGG CC^[GC]GG
NdeII 470 GATC ^GATC
NlaIV 46 GGCACC GG[ACGT]^[ACGT]CC
Sau3AI 470 GATC ^GATC
ScrFI 43 CCGGG CC^[ACGT]GG
SmlI 235 CTTGAG C^T[CT][GA]AG
Tru9I 220 TTAA T^TAA
TspRI 23 CAGTG CA[GC]TG[ACGT][ACGT]^
XmnI 494 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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AciI 10 GCGG CCGC(-3/-1)
33 GCGG
AcsI 167 AAATTC [GA]^AATT[CT]
648 GAATTC
AlwNI 302 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
653 CAGTTTCTG
ApoI 167 AAATTC [GA]^AATT[CT]
648 GAATTC
BbvI 331 GCAGC GCAGC(8/12)
394 GCTGC
BsaJI 50 CCAAGG C^C[ACGT][ACGT]GG
605 CCAAGG
DdeI 28 CTGAG C^T[ACGT]AG
575 CTAAG
EcoT14I 50 CCAAGG C^C[AT][AT]GG
605 CCAAGG
Fnu4HI 331 GCAGC GC^[ACGT]GC
394 GCTGC
HinfI 82 GAATC G^A[ACGT]TC
448 GAATC
ItaI 331 GCAGC GC^[ACGT]GC
394 GCTGC
MboII 634 GAAGA GAAGA(8/7)
402 TCTTC
MslI 291 CACTCAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
554 CATTTTTGTG
MwoI 33 GCGGAACAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
431 GCAGGGTTAGC
NspI 397 GCATGT [GA]CATG^[CT]
504 ACATGC
SfcI 362 CTATAG C^T[GA][CT]AG
455 CTGTAG
StyI 50 CCAAGG C^C[AT][AT]GG
605 CCAAGG
TfiI 82 GAATC G^A[AT]TC
448 GAATC
TseI 331 GCAGC G^C[AT]GC
394 GCTGC
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# 3 OR MORE CUT ENZYMES ( 10 enzymes) #
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AfaI AluI BfaI HaeIII MaeI
MnlI NlaIII RsaI SspI Tsp509I