Restriction map for
pFN21AE1920
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# NO CUT ENZYMES ( 127 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BanI BbeI BbrPI BciVI BclI
BfaI BfrI BglII BlnI BsaAI
BsaBI BsaHI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoT22I FbaI
FseI FspI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MaeII MamI MfeI MluI
MluNI MroI MscI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SexAI SfoI SfuI SgrAI SnaBI
SpeI SspBI SspI SwaI Tth111I
XbaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccII 6 CGCG CG^CG
AocI 1264 CCTGAGG CC^T[ACGT]AGG
ApaI 1095 GGGCCC GGGCC^C
AvaII 979 GGACC G^G[AT]CC
BanII 1095 GGGCCC G[GA]GC[CT]^C
BbsI 286 GAAGAC GAAGAC(2/6)
BcgI 1102 CGAAGTGAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 892 GCCTCGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 331 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 331 GCTCAGC GC^T[ACGT]AGC
BpuAI 286 GAAGAC GAAGAC(2/6)
BsaI 400 GAGACC GGTCTC(1/5)
BsaWI 1286 ACCGGA [AT]^CCGG[AT]
BsmBI 544 GAGACG CGTCTC(1/5)
BsmI 1108 GAATGC GAATGC(1/-1)
BspMI 692 ACCTGC ACCTGC(4/8)
BssSI 772 CACGAG CACGAG(-5/-1)
BstUI 6 CGCG CG^CG
Bsu36I 1264 CCTGAGG CC^T[ACGT]AGG
CelII 331 GCTCAGC GC^T[ACGT]AGC
CfoI 783 GCGC GCG^C
EaeI 413 TGGCCG [CT]^GGCC[GA]
Eco81I 1264 CCTGAGG CC^T[ACGT]AGG
EcoRV 172 GATATC GAT^ATC
FauI 1115 GCGGG CCCGC(4/6)
HaeII 782 GGCGCT [GA]GCGC^[CT]
HgaI 4 GACGC GACGC(5/10)
HhaI 783 GCGC GCG^C
HinP1I 783 GCGC G^CGC
MaeIII 1184 GTGAC ^GT[ACGT]AC
MseI 154 TTAA T^TAA
MvnI 6 CGCG CG^CG
NspI 578 GCATGC [GA]CATG^[CT]
PaeR7I 664 CTCGAG C^TCGAG
PflMI 1272 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 694 CTGCAG CTGCA^G
SbfI 693 CCTGCAGG CCTGCA^GG
ScaI 650 AGTACT AGT^ACT
SfiI 891 GGCCTCGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 59 CCCGGG CCC^GGG
SphI 578 GCATGC GCATG^C
Sse8387I 693 CCTGCAGG CCTGCA^GG
TfiI 477 GAATC G^A[AT]TC
Tru9I 154 TTAA T^TAA
Tsp45I 1184 GTGAC ^GT[GC]AC
Van91I 1272 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 664 CTCGAG C^TCGAG
XmaCI 59 CCCGGG C^CCGGG
XmaI 59 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 12 enzymes) #
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AlwNI 97 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
421 CAGCGGCTG
AspHI 382 GTGCTC G[AT]GC[AT]^C
1311 GTGCTC
BamHI 123 GGATCC G^GATCC
131 GGATCC
BbvCI 1209 CCTCAGC CCTCAGC(-5/-2)
823 GCTGAGG
BsgI 658 GTGCAG GTGCAG(16/14)
1354 GTGCAG
BsiHKAI 382 GTGCTC G[AT]GC[AT]^C
1311 GTGCTC
BsmFI 62 GGGAC GGGAC(10/14)
1059 GTCCC
BstAPI 420 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
465 GCAGGAGATGC
HphI 243 GGTGA GGTGA(8/7)
261 GGTGA
NcoI 569 CCATGG C^CATGG
794 CCATGG
Tsp509I 270 AATT ^AATT
1332 AATT
XcmI 521 CCAGGAGCGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
701 CCAAACTGACCCTGG
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# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaI
BbvI BcnI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmAI BsoBI
Bsp1286I BsrBI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DraII DsaI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
Ksp632I MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TseI TspRI TthHB8I XhoII