Restriction map for
pFN21AE1862
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
Acc65I AccI AccIII AclI AfaI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AseI AsnI
Asp700I Asp718I AspEI AvaI AviII
AvrII BamHI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BseAI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I CelII ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRV FbaI FseI
FspI HincII HindII HindIII HpaI
KasI KpnI MfeI MluI MroI
MunI NarI NheI NotI NruI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsaI RsrII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI StuI SwaI
TaqI TthHB8I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 235 GACGTC GACGT^C
AcyI 235 GACGTC G[GA]^CG[CT]C
AlwI 1110 GATCC GGATC(4/5)
AlwNI 256 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 907 CCTGAGG CC^T[ACGT]AGG
AscI 437 GGCGCGCC GG^CGCGCC
AspI 232 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 814 GGCACC G^G[CT][GA]CC
BfaI 457 CTAG C^TAG
BpmI 187 CTGGAG CTGGAG(16/14)
BsaBI 64 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 235 GACGTC G[GA]^CG[CT]C
BsaWI 1051 ACCGGA [AT]^CCGG[AT]
BsmFI 167 GGGAC GGGAC(10/14)
BsrDI 1031 GCAATG GCAATG(2/0)
BstYI 1109 AGATCC [GA]^GATC[CT]
Bsu36I 907 CCTGAGG CC^T[ACGT]AGG
DraII 125 AGGACCC [GA]G^G[ACGT]CC[CT]
Eco81I 907 CCTGAGG CC^T[ACGT]AGG
EcoNI 907 CCTGAGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 125 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 822 GAATTC G^AATTC
EcoT14I 779 CCATGG C^C[AT][AT]GG
EcoT22I 1 ATGCAT ATGCA^T
HaeII 419 AGCGCC [GA]GCGC^[CT]
Hin1I 235 GACGTC G[GA]^CG[CT]C
HphI 859 GGTGA GGTGA(8/7)
KspI 449 CCGCGG CCGC^GG
MaeI 457 CTAG C^TAG
MaeII 236 ACGT A^CGT
MamI 64 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1109 AGATCC [GA]^GATC[CT]
MseI 655 TTAA T^TAA
NcoI 779 CCATGG C^CATGG
NdeI 698 CATATG CA^TATG
NsiI 1 ATGCAT ATGCA^T
NspI 212 GCATGC [GA]CATG^[CT]
PflFI 232 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 125 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 709 CAGCTG CAG^CTG
SacI 1127 GAGCTC GAGCT^C
SacII 449 CCGCGG CCGC^GG
SapI 174 GCTCTTC GCTCTTC(1/4)
SmlI 610 CTTGAG C^T[CT][GA]AG
SphI 212 GCATGC GCATG^C
SspI 1084 AATATT AAT^ATT
StyI 779 CCATGG C^C[AT][AT]GG
Tru9I 655 TTAA T^TAA
Tth111I 232 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 1109 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 84 GAATTT [GA]^AATT[CT]
822 GAATTC
ApoI 84 GAATTT [GA]^AATT[CT]
822 GAATTC
AvaII 126 GGACC G^G[AT]CC
800 GGACC
BalI 887 TGGCCA TGG^CCA
930 TGGCCA
BsaI 597 GAGACC GGTCTC(1/5)
1048 GAGACC
BsgI 487 CTGCAC GTGCAG(16/14)
496 GTGCAG
BspMI 485 ACCTGC ACCTGC(4/8)
529 GCAGGT
BsrFI 278 GCCGGC [GA]^CCGG[CT]
525 GCCGGC
BssHII 308 GCGCGC G^CGCGC
438 GCGCGC
BstAPI 255 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
489 GCACGCGGTGC
BstXI 341 CCAGTGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1113 CCAGCCACATGG
Cfr10I 278 GCCGGC [GA]^CCGG[CT]
525 GCCGGC
DpnII 70 GATC ^GATC
1110 GATC
DraIII 132 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
490 CACGCGGTG
EarI 175 CTCTTC CTCTTC(1/4)
1036 GAAGAG
HgaI 376 GACGC GACGC(5/10)
474 GACGC
Ksp632I 175 CTCTTC CTCTTC(1/4)
1036 GAAGAG
MboI 70 GATC ^GATC
1110 GATC
MluNI 887 TGGCCA TGG^CCA
930 TGGCCA
MscI 887 TGGCCA TGG^CCA
930 TGGCCA
MslI 75 CATGTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
842 CATCCTTGTG
MspA1I 449 CCGCGG C[AC]G^C[GT]G
709 CAGCTG
NaeI 278 GCCGGC GCC^GGC
525 GCCGGC
NdeII 70 GATC ^GATC
1110 GATC
NgoMIV 278 GCCGGC G^CCGGC
525 GCCGGC
Sau3AI 70 GATC ^GATC
1110 GATC
SfaNI 3 GCATC GCATC(5/9)
588 GCATC
Tsp509I 85 AATT ^AATT
823 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AccII AciI AluI AspHI BanII
BbvI BcnI BmyI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DsaI
EaeI EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI MaeIII MboII MnlI
MspI MvaI MvnI MwoI NciI
NlaIII NlaIV PleI PstI Sau96I
ScrFI SfcI TfiI TseI Tsp45I
TspRI XcmI