Restriction map for
pFN21AE1413
##################################
# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI AspEI AspI AviII
AvrII BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaWI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII CfoI ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HaeII HhaI HinP1I HincII
HindII HindIII HpaI KasI KspI
MaeII MamI MfeI MluI MroI
MunI MvnI NarI NciI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tth111I Van91I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 1074 GGTACC G^GTACC
AcsI 1249 GAATTC [GA]^AATT[CT]
AcyI 766 GACGCC G[GA]^CG[CT]C
ApoI 1249 GAATTC [GA]^AATT[CT]
Asp718I 1074 GGTACC G^GTACC
AspHI 778 GAGCTC G[AT]GC[AT]^C
AvaI 547 CTCGGG C^[CT]CG[GA]G
BamHI 96 GGATCC G^GATCC
BanI 1074 GGTACC G^G[CT][GA]CC
BfaI 832 CTAG C^TAG
BsaHI 766 GACGCC G[GA]^CG[CT]C
BsaI 490 GAGACC GGTCTC(1/5)
BsgI 1417 GTGCAG GTGCAG(16/14)
BsiHKAI 778 GAGCTC G[AT]GC[AT]^C
BsmI 711 GAATGC GAATGC(1/-1)
BsoBI 547 CTCGGG C^[CT]CG[GA]G
Bsp1407I 728 TGTACA T^GTACA
BsrDI 475 GCAATG GCAATG(2/0)
BsrFI 824 GCCGGC [GA]^CCGG[CT]
BsrGI 728 TGTACA T^GTACA
BstXI 1382 CCATCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 824 GCCGGC [GA]^CCGG[CT]
EarI 1432 CTCTTC CTCTTC(1/4)
EcoNI 531 CCTTACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1249 GAATTC G^AATTC
HapII 825 CCGG C^CGG
HgaI 766 GACGC GACGC(5/10)
Hin1I 766 GACGCC G[GA]^CG[CT]C
HpaII 825 CCGG C^CGG
KpnI 1074 GGTACC GGTAC^C
Ksp632I 1432 CTCTTC CTCTTC(1/4)
MaeI 832 CTAG C^TAG
MseI 1274 TTAA T^TAA
MspA1I 1036 CAGCTG C[AC]G^C[GT]G
MspI 825 CCGG C^CGG
NaeI 824 GCCGGC GCC^GGC
NgoMIV 824 GCCGGC G^CCGGC
PvuII 1036 CAGCTG CAG^CTG
SacI 778 GAGCTC GAGCT^C
SspBI 728 TGTACA T^GTACA
TaqI 1256 TCGA T^CGA
TfiI 334 GATTC G^A[AT]TC
Tru9I 1274 TTAA T^TAA
TthHB8I 1256 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
Asp700I 630 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
931 GAACCCTTTC
BalI 689 TGGCCA TGG^CCA
797 TGGCCA
BanII 277 GAGCCC G[GA]GC[CT]^C
778 GAGCTC
BglII 668 AGATCT A^GATCT
1302 AGATCT
BseRI 60 CTCCTC GAGGAG(10/8)
865 CTCCTC
BsmFI 698 GGGAC GGGAC(10/14)
1445 GGGAC
EaeI 689 TGGCCA [CT]^GGCC[GA]
797 TGGCCA
EcoT14I 599 CCAAGG C^C[AT][AT]GG
1025 CCAAGG
MluNI 689 TGGCCA TGG^CCA
797 TGGCCA
MscI 689 TGGCCA TGG^CCA
797 TGGCCA
PleI 402 GAGTC GAGTC(4/5)
746 GAGTC
PstI 1039 CTGCAG CTGCA^G
1370 CTGCAG
SfcI 1039 CTGCAG C^T[GA][CT]AG
1370 CTGCAG
StyI 599 CCAAGG C^C[AT][AT]GG
1025 CCAAGG
XcmI 345 CCAGCCCACTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
566 CCAAAACAAACCTGG
XmnI 630 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
931 GAACCCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BbsI BbvI BmyI BpmI
BpuAI BsaJI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeIII MboI MboII MflI MnlI
MslI MvaI MwoI NdeII NlaIII
NlaIV PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI TseI Tsp45I Tsp509I
TspRI XhoII