Restriction map for
pFN21AE1916
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# NO CUT ENZYMES ( 115 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AgeI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI Asp700I
Asp718I AspHI AvrII BalI BamHI
BanII BbeI BbrPI BbvCI BciVI
BfrI BlnI BlpI BmyI Bpu1102I
BsaAI BsaBI BsaHI BseAI BsiEI
BsiHKAI BsiWI BsmBI Bsp1286I BspDI
BspEI BspLU11I BsrBI Bst1107I BstBI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DrdI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT22I FseI HgaI Hin1I
HincII HindII HpaI KasI KpnI
MamI MfeI MluI MluNI MroI
MscI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflMI PinAI PmaCI
PmeI PmlI Psp1406I PvuI PvuII
RsrII SacI SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SwaI
TfiI Van91I XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AflIII 737 ACGTGT A^C[GA][CT]GT
AhdI 928 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 928 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1161 CTCGAG C^[CT]CG[GA]G
AviII 1004 TGCGCA TGC^GCA
BanI 1289 GGCACC G^G[CT][GA]CC
BbsI 440 GAAGAC GAAGAC(2/6)
BcgI 734 GCAACGTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 579 TGATCA T^GATCA
BglI 26 GCCCCGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 138 AGATCT A^GATCT
BpuAI 440 GAAGAC GAAGAC(2/6)
BsaWI 261 ACCGGA [AT]^CCGG[AT]
BsgI 1276 CTGCAC GTGCAG(16/14)
BsmI 333 GAATGC GAATGC(1/-1)
BsoBI 1161 CTCGAG C^[CT]CG[GA]G
Bsp1407I 1334 TGTACA T^GTACA
BsrDI 1308 CATTGC GCAATG(2/0)
BsrFI 972 GCCGGT [GA]^CCGG[CT]
BsrGI 1334 TGTACA T^GTACA
BssHII 1182 GCGCGC G^CGCGC
BssSI 994 CACGAG CACGAG(-5/-1)
BstAPI 1010 GCAAGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 320 GGTTACC G^GT[ACGT]ACC
BstPI 320 GGTTACC G^GT[ACGT]ACC
Cfr10I 972 GCCGGT [GA]^CCGG[CT]
DraIII 1291 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 92 TGGCCG [CT]^GGCC[GA]
Eam1105I 928 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 826 GAAGAG CTCTTC(1/4)
EcoO65I 320 GGTTACC G^GT[ACGT]ACC
EcoT14I 494 CCATGG C^C[AT][AT]GG
FbaI 579 TGATCA T^GATCA
FspI 1004 TGCGCA TGC^GCA
HindIII 648 AAGCTT A^AGCTT
HinfI 516 GAGTC G^A[ACGT]TC
HphI 939 GGTGA GGTGA(8/7)
Ksp632I 826 GAAGAG CTCTTC(1/4)
KspI 29 CCGCGG CCGC^GG
MslI 1308 CATTGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 29 CCGCGG C[AC]G^C[GT]G
NcoI 494 CCATGG C^CATGG
NspI 782 ACATGC [GA]CATG^[CT]
PaeR7I 1161 CTCGAG C^TCGAG
PleI 516 GAGTC GAGTC(4/5)
PshAI 693 GACCGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 29 CCGCGG CCGC^GG
SapI 826 GAAGAGC GCTCTTC(1/4)
SexAI 986 ACCAGGT A^CC[AT]GGT
SfaNI 1040 GCATC GCATC(5/9)
SmlI 1161 CTCGAG C^T[CT][GA]AG
SspBI 1334 TGTACA T^GTACA
SspI 483 AATATT AAT^ATT
StuI 1157 AGGCCT AGG^CCT
StyI 494 CCATGG C^C[AT][AT]GG
XbaI 285 TCTAGA T^CTAGA
XcmI 494 CCATGGATCTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1161 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccI 354 GTCTAC GT^[AC][GT]AC
745 GTCTAC
AseI 304 ATTAAT AT^TAAT
616 ATTAAT
AsnI 304 ATTAAT AT^TAAT
616 ATTAAT
AspI 1092 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1300 GACAATGTC
BcnI 1094 CCGGG CC^[GC]GG
1199 CCCGG
BfaI 218 CTAG C^TAG
286 CTAG
BpmI 134 CTGGAG CTGGAG(16/14)
1153 CTGGAG
BsaI 449 GAGACC GGTCTC(1/5)
979 GAGACC
BseRI 789 CTCCTC GAGGAG(10/8)
685 GAGGAG
BspMI 1001 ACCTGC ACCTGC(4/8)
63 GCAGGT
BsrI 48 CCAGT ACTGG(1/-1)
729 CCAGT
HaeII 468 GGCGCT [GA]GCGC^[CT]
1081 AGCGCC
MaeI 218 CTAG C^TAG
286 CTAG
MaeII 89 ACGT A^CGT
737 ACGT
NciI 1094 CCGGG CC^[GC]GG
1199 CCCGG
PflFI 1092 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1300 GACAATGTC
PshBI 304 ATTAAT AT^TAAT
616 ATTAAT
PstI 599 CTGCAG CTGCA^G
707 CTGCAG
Tsp45I 779 GTGAC ^GT[GC]AC
1147 GTGAC
Tth111I 1092 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1300 GACAATGTC
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# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AvaII BbvI BsaJI
BsiYI BslI BsmAI BsmFI BspHI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII ItaI MaeIII MboI
MboII MflI MnlI MseI MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV PpuMI RcaI RsaI Sau3AI
Sau96I ScrFI SfcI TaqI Tru9I
TseI Tsp509I TspRI TthHB8I XhoII