Restriction map for
pFN21AE1958
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I AocI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AviII BbrPI
BbsI BclI BfrI BglII BpuAI
BsaAI BsaBI BsaWI BseAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BsrGI BssHII BssSI BstAPI
BstBI Bsu36I ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco52I Eco81I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HpaI KpnI KspI MamI
MfeI MluI MroI MslI MunI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI Tsp509I Tth111I TthHB8I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccI 410 GTATAC GT^[AC][GT]AC
AfaI 1133 GTAC GT^AC
Aor51HI 808 AGCGCT AGC^GCT
Asp700I 1476 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 1464 CCTAGG C^CTAGG
BamHI 1375 GGATCC G^GATCC
BbvCI 1071 GCTGAGG CCTCAGC(-5/-2)
BciVI 673 GTATCC GTATCC(6/5)
BlnI 1464 CCTAGG C^CTAGG
BsaI 480 GGTCTC GGTCTC(1/5)
BspLU11I 1129 ACATGT A^CATGT
BsrBI 754 GAGCGG CCGCTC(-3/-3)
BsrDI 791 CATTGC GCAATG(2/0)
Bst1107I 410 GTATAC GTA^TAC
BstEII 1105 GGTGACC G^GT[ACGT]ACC
BstPI 1105 GGTGACC G^GT[ACGT]ACC
BstXI 1293 CCAGACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 410 GTATAC GTA^TAC
EarI 1391 CTCTTC CTCTTC(1/4)
Eco47III 808 AGCGCT AGC^GCT
EcoNI 415 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1105 GGTGACC G^GT[ACGT]ACC
HgaI 610 GCGTC GACGC(5/10)
HindIII 1041 AAGCTT A^AGCTT
Ksp632I 1391 CTCTTC CTCTTC(1/4)
MspA1I 1069 CAGCTG C[AC]G^C[GT]G
NaeI 559 GCCGGC GCC^GGC
NgoMIV 559 GCCGGC G^CCGGC
PpuMI 706 GGGTCCC [GA]G^G[AT]CC[CT]
PvuI 698 CGATCG CGAT^CG
PvuII 1069 CAGCTG CAG^CTG
RsaI 1133 GTAC GT^AC
SacI 117 GAGCTC GAGCT^C
SfaNI 291 GCATC GCATC(5/9)
SfiI 570 GGCCGCCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 423 AGGCCT AGG^CCT
XmnI 1476 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflIII 209 ACGTGT A^C[GA][CT]GT
1129 ACATGT
BalI 267 TGGCCA TGG^CCA
1290 TGGCCA
BcgI 925 CGACAGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
692 GCACCGCGATCG
BglI 487 GCCCGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
571 GCCGCCTGGGC
BlpI 804 GCTGAGC GC^T[ACGT]AGC
915 GCTAAGC
Bpu1102I 804 GCTGAGC GC^T[ACGT]AGC
915 GCTAAGC
BsgI 886 GTGCAG GTGCAG(16/14)
1003 GTGCAG
BsiEI 614 CGGTCG CG[GA][CT]^CG
698 CGATCG
BsmAI 1057 GAGAC GTCTC(1/5)
481 GTCTC
BspMI 414 ACCTGC ACCTGC(4/8)
1224 GCAGGT
BsrFI 559 GCCGGC [GA]^CCGG[CT]
1109 ACCGGC
BstF5I 340 CATCC GGATG(2/0)
273 GGATG
BstYI 536 GGATCT [GA]^GATC[CT]
1375 GGATCC
CelII 804 GCTGAGC GC^T[ACGT]AGC
915 GCTAAGC
Cfr10I 559 GCCGGC [GA]^CCGG[CT]
1109 ACCGGC
FokI 340 CATCC GGATG(9/13)
273 GGATG
MaeII 209 ACGT A^CGT
668 ACGT
MboII 1392 TCTTC GAAGA(8/7)
1481 TCTTC
MflI 536 GGATCT [GA]^GATC[CT]
1375 GGATCC
MluNI 267 TGGCCA TGG^CCA
1290 TGGCCA
MscI 267 TGGCCA TGG^CCA
1290 TGGCCA
NspI 1129 ACATGT [GA]CATG^[CT]
1347 GCATGT
PleI 56 GACTC GAGTC(4/5)
1447 GACTC
PstI 158 CTGCAG CTGCA^G
862 CTGCAG
SmlI 782 CTCAAG C^T[CT][GA]AG
1504 CTCAAG
TfiI 1164 GAATC G^A[AT]TC
1384 GAATC
Tsp45I 1106 GTGAC ^GT[GC]AC
1153 GTCAC
XcmI 169 CCAAAAGTGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
255 CCAACATTGGGTTGG
XhoII 536 GGATCT [GA]^GATC[CT]
1375 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BanI BanII BbeI BbvI BcnI
BfaI BmyI BpmI BsaHI BsaJI
BseRI BsiHKAI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrI BssKI BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI MaeI
MaeIII MboI MnlI MseI MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV PflMI Sau3AI
Sau96I ScrFI SfcI SfoI StyI
Tru9I TseI TspRI Van91I