Restriction map for
pFN21AE0905
##################################
# NO CUT ENZYMES ( 170 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspI AviII
AvrII BalI BamHI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BpuAI
BsaAI BsaBI BsaHI BsaWI BseAI
BsgI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EaeI EagI EarI
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HaeIII Hin1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MamI MfeI
MflI MluI MluNI MroI MscI
MseI MslI MunI MwoI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfaNI SfcI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI Tru9I Tsp509I Tth111I
Van91I XbaI XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AfaI 339 GTAC GT^AC
AhdI 478 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 478 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 511 GAGCTC G[AT]GC[AT]^C
AvaI 388 CCCGGG C^[CT]CG[GA]G
AvaII 477 GGACC G^G[AT]CC
BanI 57 GGTGCC G^G[CT][GA]CC
BanII 511 GAGCTC G[GA]GC[CT]^C
BfaI 469 CTAG C^TAG
BlpI 45 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 45 GCTGAGC GC^T[ACGT]AGC
BsaI 85 GGTCTC GGTCTC(1/5)
BseRI 127 CTCCTC GAGGAG(10/8)
BsiHKAI 511 GAGCTC G[AT]GC[AT]^C
BsmBI 334 GAGACG CGTCTC(1/5)
BsoBI 388 CCCGGG C^[CT]CG[GA]G
BsrBI 352 CCGCTC CCGCTC(-3/-3)
BstF5I 150 CATCC GGATG(2/0)
CelII 45 GCTGAGC GC^T[ACGT]AGC
CfoI 72 GCGC GCG^C
Cfr13I 477 GGACC G^G[ACGT]CC
DpnII 189 GATC ^GATC
Eam1105I 478 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 309 CCTAACTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 68 GCGGG CCCGC(4/6)
FokI 150 CATCC GGATG(9/13)
HaeII 71 GGCGCT [GA]GCGC^[CT]
HapII 389 CCGG C^CGG
HgaI 29 GCGTC GACGC(5/10)
HhaI 72 GCGC GCG^C
HinP1I 72 GCGC G^CGC
HpaII 389 CCGG C^CGG
HphI 180 TCACC GGTGA(8/7)
MaeI 469 CTAG C^TAG
MaeIII 454 GTGAC ^GT[ACGT]AC
MboI 189 GATC ^GATC
MspI 389 CCGG C^CGG
NdeII 189 GATC ^GATC
NlaIII 133 CATG CATG^
PleI 445 GACTC GAGTC(4/5)
PvuII 206 CAGCTG CAG^CTG
RsaI 339 GTAC GT^AC
SacI 511 GAGCTC GAGCT^C
Sau3AI 189 GATC ^GATC
Sau96I 477 GGACC G^G[ACGT]CC
SmaI 388 CCCGGG CCC^GGG
SmlI 355 CTCAAG C^T[CT][GA]AG
TaqI 515 TCGA T^CGA
Tsp45I 454 GTGAC ^GT[GC]AC
TspRI 22 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 515 TCGA T^CGA
XcmI 497 CCACAACGTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 388 CCCGGG C^CCGGG
XmaI 388 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccII 33 CGCG CG^CG
54 CGCG
BbvI 3 GCAGC GCAGC(8/12)
348 GCAGC
BcnI 388 CCCGG CC^[GC]GG
389 CCGGG
BmyI 58 GTGCCC G[AGT]GC[ACT]^C
511 GAGCTC
BpmI 449 CTGGAG CTGGAG(16/14)
508 CTGGAG
BsmFI 476 GGGAC GGGAC(10/14)
234 GTCCC
Bsp1286I 58 GTGCCC G[AGT]GC[ACT]^C
511 GAGCTC
BsrI 12 ACTGG ACTGG(1/-1)
457 ACTGG
BstNI 364 CCTGG CC^[AT]GG
437 CCTGG
BstUI 33 CGCG CG^CG
54 CGCG
Cac8I 68 GCGGGC GC[ACGT]^[ACGT]GC
383 GCAAGC
EcoRII 364 CCTGG ^CC[AT]GG
437 CCTGG
MaeII 337 ACGT A^CGT
502 ACGT
MboII 369 GAAGA GAAGA(8/7)
173 TCTTC
MspA1I 4 CAGCGG C[AC]G^C[GT]G
206 CAGCTG
MvaI 364 CCTGG CC^[AT]GG
437 CCTGG
MvnI 33 CGCG CG^CG
54 CGCG
NciI 388 CCCGG CC^[GC]GG
389 CCGGG
NlaIV 57 GGTGCC GG[ACGT]^[ACGT]CC
476 GGGACC
TfiI 170 GATTC G^A[AT]TC
517 GATTC
TseI 3 GCAGC G^C[AT]GC
348 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 13 enzymes) #
#########################################
AciI AluI BsaJI BsiYI BslI
BsmAI BssKI DdeI Fnu4HI HinfI
ItaI MnlI ScrFI