Restriction map for
pFN21AE0933
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# NO CUT ENZYMES ( 146 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
AocI Aor51HI ApaI AscI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanII BbeI BbrPI
BbvCI BcgI BcnI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmAI BsmBI
BsmI BsoBI BspDI BspEI BspHI
BspLU11I BspMI BsrDI BsrFI BssHII
BssSI BstAPI BstF5I BstUI BstXI
BstYI Bsu36I CelII Cfr10I ClaI
CpoI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoRI EcoRV EcoT22I
FokI FseI FspI HapII Hin1I
HindIII HpaI HpaII KasI KpnI
KspI MamI MfeI MflI MluI
MroI MspI MunI MvnI NaeI
NarI NciI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tth111I
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcsI 71 AAATTC [GA]^AATT[CT]
Alw44I 368 GTGCAC G^TGCAC
AlwI 639 GATCC GGATC(4/5)
AlwNI 55 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 368 GTGCAC G^TGCAC
ApoI 71 AAATTC [GA]^AATT[CT]
AseI 205 ATTAAT AT^TAAT
AsnI 205 ATTAAT AT^TAAT
AvaII 262 GGTCC G^G[AT]CC
BanI 670 GGCACC G^G[CT][GA]CC
BciVI 36 GGATAC GTATCC(6/5)
BsaJI 624 CCATGG C^C[ACGT][ACGT]GG
BseRI 1024 GAGGAG GAGGAG(10/8)
Bsp1407I 988 TGTACA T^GTACA
BsrBI 16 GAGCGG CCGCTC(-3/-3)
BsrGI 988 TGTACA T^GTACA
BssKI 536 CCTGG ^CC[ACGT]GG
Bst1107I 851 GTATAC GTA^TAC
BstEII 979 GGTGACC G^GT[ACGT]ACC
BstNI 536 CCTGG CC^[AT]GG
BstPI 979 GGTGACC G^GT[ACGT]ACC
BstZ17I 851 GTATAC GTA^TAC
DsaI 624 CCATGG C^C[GA][CT]GG
EcoO65I 979 GGTGACC G^GT[ACGT]ACC
EcoRII 536 CCTGG ^CC[AT]GG
EcoT14I 624 CCATGG C^C[AT][AT]GG
FauI 18 GCGGG CCCGC(4/6)
HgaI 45 GCGTC GACGC(5/10)
HincII 220 GTTGAC GT[CT]^[GA]AC
HindII 220 GTTGAC GT[CT]^[GA]AC
MaeII 1053 ACGT A^CGT
MspA1I 58 CCGCTG C[AC]G^C[GT]G
MvaI 536 CCTGG CC^[AT]GG
NcoI 624 CCATGG C^CATGG
NdeI 158 CATATG CA^TATG
NspI 277 ACATGC [GA]CATG^[CT]
PflMI 541 CCAGATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 688 GACACTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 205 ATTAAT AT^TAAT
ScaI 374 AGTACT AGT^ACT
ScrFI 536 CCTGG CC^[ACGT]GG
SfaNI 399 GATGC GCATC(5/9)
SfcI 645 CTGTAG C^T[GA][CT]AG
SspBI 988 TGTACA T^GTACA
StyI 624 CCATGG C^C[AT][AT]GG
Tsp45I 980 GTGAC ^GT[GC]AC
Van91I 541 CCAGATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 957 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 27 enzymes) #
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AccI 685 GTAGAC GT^[AC][GT]AC
851 GTATAC
AspHI 368 GTGCAC G[AT]GC[AT]^C
1040 GTGCTC
BbsI 312 GAAGAC GAAGAC(2/6)
883 GAAGAC
BclI 162 TGATCA T^GATCA
903 TGATCA
BfaI 848 CTAG C^TAG
958 CTAG
BmyI 368 GTGCAC G[AGT]GC[ACT]^C
1040 GTGCTC
BpuAI 312 GAAGAC GAAGAC(2/6)
883 GAAGAC
BsgI 650 GTGCAG GTGCAG(16/14)
1093 GTGCAG
BsiHKAI 368 GTGCAC G[AT]GC[AT]^C
1040 GTGCTC
BsmFI 84 GTCCC GGGAC(10/14)
553 GTCCC
Bsp1286I 368 GTGCAC G[AGT]GC[ACT]^C
1040 GTGCTC
BsrI 377 ACTGG ACTGG(1/-1)
212 CCAGT
BstBI 25 TTCGAA TT^CGAA
74 TTCGAA
CfoI 663 GCGC GCG^C
1202 GCGC
Cfr13I 262 GGTCC G^G[ACGT]CC
628 GGGCC
DraI 406 TTTAAA TTT^AAA
589 TTTAAA
EarI 28 GAAGAG CTCTTC(1/4)
229 GAAGAG
FbaI 162 TGATCA T^GATCA
903 TGATCA
HaeII 662 GGCGCT [GA]GCGC^[CT]
1201 GGCGCT
HhaI 663 GCGC GCG^C
1202 GCGC
HinP1I 663 GCGC G^CGC
1202 GCGC
Ksp632I 28 GAAGAG CTCTTC(1/4)
229 GAAGAG
MaeI 848 CTAG C^TAG
958 CTAG
NspV 25 TTCGAA TT^CGAA
74 TTCGAA
PleI 82 GAGTC GAGTC(4/5)
551 GAGTC
Sau96I 262 GGTCC G^G[ACGT]CC
628 GGGCC
SfuI 25 TTCGAA TT^CGAA
74 TTCGAA
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# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AfaI AluI BalI BbvI
BsiYI BslI Cac8I DdeI DpnII
EaeI Fnu4HI HaeIII HinfI HphI
ItaI MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I