Restriction map for
pFN21AE1736
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# NO CUT ENZYMES ( 156 enzymes) #
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AccI AclI AflII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI AspEI AspHI
AspI AviII AvrII BamHI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI BmyI Bpu1102I BsaAI
BsaBI BsaI BsaJI BsiEI BsiHKAI
BsiWI BsiYI BslI BsmBI Bsp1286I
Bsp1407I BspDI BsrBI BsrFI BsrGI
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I CelII
Cfr10I ClaI CpoI DraII DraIII
DrdI DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HaeII HgaI
HincII HindII HindIII HpaI HphI
KasI Ksp632I KspI MaeI MamI
MfeI MflI MluI MspA1I MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 72 enzymes) #
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AatII 578 GACGTC GACGT^C
Acc65I 480 GGTACC G^GTACC
AccII 51 CGCG CG^CG
AccIII 445 TCCGGA T^CCGGA
AciI 47 GCGG CCGC(-3/-1)
AcsI 200 GAATTC [GA]^AATT[CT]
AcyI 578 GACGTC G[GA]^CG[CT]C
AflIII 215 ACATGT A^C[GA][CT]GT
AocI 502 CCTGAGG CC^T[ACGT]AGG
ApoI 200 GAATTC [GA]^AATT[CT]
Asp700I 196 GAACGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 480 GGTACC G^GTACC
AvaI 17 CTCGGG C^[CT]CG[GA]G
AvaII 253 GGACC G^G[AT]CC
BalI 368 TGGCCA TGG^CCA
BanI 480 GGTACC G^G[CT][GA]CC
BbsI 211 GAAGAC GAAGAC(2/6)
BbvI 358 GCTGC GCAGC(8/12)
BcnI 38 CCCGG CC^[GC]GG
BpmI 131 CTGGAG CTGGAG(16/14)
BpuAI 211 GAAGAC GAAGAC(2/6)
BsaHI 578 GACGTC G[GA]^CG[CT]C
BsaWI 445 TCCGGA [AT]^CCGG[AT]
BseAI 445 TCCGGA T^CCGGA
BsmAI 26 GAGAC GTCTC(1/5)
BsmFI 127 GGGAC GGGAC(10/14)
BsmI 306 GAATGC GAATGC(1/-1)
BsoBI 17 CTCGGG C^[CT]CG[GA]G
BspEI 445 TCCGGA T^CCGGA
BspHI 302 TCATGA T^CATGA
BspLU11I 215 ACATGT A^CATGT
BspMI 328 GCAGGT ACCTGC(4/8)
BssHII 50 GCGCGC G^CGCGC
BstF5I 67 CATCC GGATG(2/0)
BstNI 385 CCAGG CC^[AT]GG
BstUI 51 CGCG CG^CG
Bsu36I 502 CCTGAGG CC^T[ACGT]AGG
Cac8I 50 GCGCGC GC[ACGT]^[ACGT]GC
Cfr13I 253 GGACC G^G[ACGT]CC
DdeI 503 CTGAG C^T[ACGT]AG
DraI 397 TTTAAA TTT^AAA
EaeI 368 TGGCCA [CT]^GGCC[GA]
Eco81I 502 CCTGAGG CC^T[ACGT]AGG
EcoRI 200 GAATTC G^AATTC
EcoRII 385 CCAGG ^CC[AT]GG
FokI 67 CATCC GGATG(9/13)
Hin1I 578 GACGTC G[GA]^CG[CT]C
KpnI 480 GGTACC GGTAC^C
MaeII 579 ACGT A^CGT
MaeIII 121 GTCAC ^GT[ACGT]AC
MluNI 368 TGGCCA TGG^CCA
MroI 445 TCCGGA T^CCGGA
MscI 368 TGGCCA TGG^CCA
MseI 398 TTAA T^TAA
MslI 154 CATGGCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 385 CCAGG CC^[AT]GG
MvnI 51 CGCG CG^CG
MwoI 361 GCTGTGATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 38 CCCGG CC^[GC]GG
NlaIV 480 GGTACC GG[ACGT]^[ACGT]CC
NspI 215 ACATGT [GA]CATG^[CT]
PleI 556 GACTC GAGTC(4/5)
RcaI 302 TCATGA T^CATGA
Sau96I 253 GGACC G^G[ACGT]CC
ScaI 567 AGTACT AGT^ACT
TaqI 265 TCGA T^CGA
TfiI 339 GATTC G^A[AT]TC
Tru9I 398 TTAA T^TAA
TseI 358 GCTGC G^C[AT]GC
Tsp45I 121 GTCAC ^GT[GC]AC
TthHB8I 265 TCGA T^CGA
XmnI 196 GAACGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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AlwI 81 GATCC GGATC(4/5)
688 GATCC
BsgI 332 GTGCAG GTGCAG(16/14)
563 GTGCAG
BsrDI 171 CATTGC GCAATG(2/0)
538 GCAATG
BssKI 38 CCCGG ^CC[ACGT]GG
385 CCAGG
CfoI 50 GCGC GCG^C
52 GCGC
DpnII 81 GATC ^GATC
688 GATC
Fnu4HI 47 GCGGC GC^[ACGT]GC
358 GCTGC
HaeIII 369 GGCC GG^CC
618 GGCC
HapII 39 CCGG C^CGG
446 CCGG
HhaI 50 GCGC GCG^C
52 GCGC
HinP1I 50 GCGC G^CGC
52 GCGC
HinfI 339 GATTC G^A[ACGT]TC
556 GACTC
HpaII 39 CCGG C^CGG
446 CCGG
ItaI 47 GCGGC GC^[ACGT]GC
358 GCTGC
MboI 81 GATC ^GATC
688 GATC
MspI 39 CCGG C^CGG
446 CCGG
NdeII 81 GATC ^GATC
688 GATC
Sau3AI 81 GATC ^GATC
688 GATC
ScrFI 38 CCCGG CC^[ACGT]GG
385 CCAGG
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# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
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AfaI AluI BseRI BsrI MboII
MnlI NlaIII RsaI SfaNI Tsp509I
TspRI