Restriction map for
pFN21AB8975
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbrPI BbsI
BbvCI BcgI BclI BfaI BfrI
BlnI BpuAI BsaAI BsaBI BsaI
BsgI BsiWI BsmBI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrDI BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
DraII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI MaeI MamI MfeI
MseI MunI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SalI SbfI ScaI
SexAI SfaNI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
Tsp509I Tth111I Van91I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccIII 981 TCCGGA T^CCGGA
AflIII 768 ACGCGT A^C[GA][CT]GT
AlwI 591 GATCC GGATC(4/5)
ApaI 387 GGGCCC GGGCC^C
BalI 581 TGGCCA TGG^CCA
BciVI 1030 GTATCC GTATCC(6/5)
BglII 617 AGATCT A^GATCT
BlpI 748 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 748 GCTCAGC GC^T[ACGT]AGC
BsaWI 981 TCCGGA [AT]^CCGG[AT]
BseAI 981 TCCGGA T^CCGGA
BseRI 1053 CTCCTC GAGGAG(10/8)
BsiEI 1101 CGGTCG CG[GA][CT]^CG
BsmAI 978 GTCTC GTCTC(1/5)
BspEI 981 TCCGGA T^CCGGA
BsrI 975 CCAGT ACTGG(1/-1)
BssHII 473 GCGCGC G^CGCGC
BstF5I 334 GGATG GGATG(2/0)
BstXI 908 CCATCTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 617 AGATCT [GA]^GATC[CT]
CelII 748 GCTCAGC GC^T[ACGT]AGC
EarI 499 CTCTTC CTCTTC(1/4)
FokI 334 GGATG GGATG(9/13)
Ksp632I 499 CTCTTC CTCTTC(1/4)
KspI 1010 CCGCGG CCGC^GG
MaeII 443 ACGT A^CGT
MaeIII 190 GTCAC ^GT[ACGT]AC
MboII 500 TCTTC GAAGA(8/7)
MflI 617 AGATCT [GA]^GATC[CT]
MluI 768 ACGCGT A^CGCGT
MluNI 581 TGGCCA TGG^CCA
MroI 981 TCCGGA T^CCGGA
MscI 581 TGGCCA TGG^CCA
NaeI 931 GCCGGC GCC^GGC
NgoMIV 931 GCCGGC G^CCGGC
SacII 1010 CCGCGG CCGC^GG
SapI 498 GCTCTTC GCTCTTC(1/4)
SmaI 948 CCCGGG CCC^GGG
SmlI 48 CTTGAG C^T[CT][GA]AG
TaqI 4 TCGA T^CGA
Tsp45I 190 GTCAC ^GT[GC]AC
TspRI 35 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 4 TCGA T^CGA
XhoII 617 AGATCT [GA]^GATC[CT]
XmaCI 948 CCCGGG C^CCGGG
XmaI 948 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwNI 35 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
679 CAGCAACTG
AspHI 496 GTGCTC G[AT]GC[AT]^C
574 GAGCAC
BglI 842 GCCTACAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
886 GCCGCGCAGGC
BpmI 58 CTGGAG CTGGAG(16/14)
526 CTGGAG
BsiHKAI 496 GTGCTC G[AT]GC[AT]^C
574 GAGCAC
BspMI 513 GCAGGT ACCTGC(4/8)
609 GCAGGT
BsrBI 396 GAGCGG CCGCTC(-3/-3)
411 GAGCGG
BsrFI 780 GCCGGT [GA]^CCGG[CT]
931 GCCGGC
Cfr10I 780 GCCGGT [GA]^CCGG[CT]
931 GCCGGC
DdeI 33 CTCAG C^T[ACGT]AG
749 CTCAG
DpnII 591 GATC ^GATC
618 GATC
DraIII 166 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
723 CACCGGGTG
EcoT14I 659 CCAAGG C^C[AT][AT]GG
936 CCTTGG
HphI 789 GGTGA GGTGA(8/7)
191 TCACC
MboI 591 GATC ^GATC
618 GATC
MslI 402 CATGAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
909 CATCTCCGTG
NdeII 591 GATC ^GATC
618 GATC
PstI 685 CTGCAG CTGCA^G
1018 CTGCAG
Sau3AI 591 GATC ^GATC
618 GATC
SfcI 685 CTGCAG C^T[GA][CT]AG
1018 CTGCAG
StyI 659 CCAAGG C^C[AT][AT]GG
936 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaI AvaII BanI BanII BbeI
BbvI BcnI BmyI BsaHI BsaJI
BsiYI BslI BsmFI BsoBI Bsp1286I
BssKI BstAPI BstNI BstUI Cac8I
CfoI Cfr13I DsaI EaeI EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII ItaI KasI MnlI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NlaIII NlaIV PleI RsaI
Sau96I ScrFI SfoI TseI