Restriction map for
pFN21AB8350
##################################
# NO CUT ENZYMES ( 129 enzymes) #
##################################
Acc65I AccI AclI AfaI AflII
AflIII AgeI AhdI AlwNI AocI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BalI BamHI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsgI BsiWI BsmBI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrDI BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DdeI DraI DsaI EagI Eam1105I
EclXI Eco52I Eco81I EcoO65I EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MaeI MamI MfeI MluI MluNI
MscI MseI MslI MunI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsaI RsrII SacII SalI
ScaI SexAI SfiI SfuI SmaI
SnaBI SpeI SphI SspBI SspI
StuI SwaI Tru9I Tth111I Van91I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AatII 494 GACGTC GACGT^C
AccIII 635 TCCGGA T^CCGGA
AcsI 520 GAATTC [GA]^AATT[CT]
Alw44I 148 GTGCAC G^TGCAC
AlwI 388 GATCC GGATC(4/5)
Aor51HI 327 AGCGCT AGC^GCT
ApaI 414 GGGCCC GGGCC^C
ApaLI 148 GTGCAC G^TGCAC
ApoI 520 GAATTC [GA]^AATT[CT]
AvaI 298 CCCGAG C^[CT]CG[GA]G
AvaII 105 GGACC G^G[AT]CC
BbeI 21 GGCGCC GGCGC^C
BglI 358 GCCAACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 656 GAGACC GGTCTC(1/5)
BsaWI 635 TCCGGA [AT]^CCGG[AT]
BseAI 635 TCCGGA T^CCGGA
BseRI 448 CTCCTC GAGGAG(10/8)
BsiEI 402 CGGTCG CG[GA][CT]^CG
BsmFI 492 GGGAC GGGAC(10/14)
BsoBI 298 CCCGAG C^[CT]CG[GA]G
BspEI 635 TCCGGA T^CCGGA
BsrI 723 CCAGT ACTGG(1/-1)
BssHII 244 GCGCGC G^CGCGC
BstAPI 141 GCACGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 240 GGATG GGATG(2/0)
BstXI 230 CCAGCTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 387 AGATCC [GA]^GATC[CT]
DraII 413 AGGGCCC [GA]G^G[ACGT]CC[CT]
DraIII 142 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 459 GACCAAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 198 CTCTTC CTCTTC(1/4)
Eco47III 327 AGCGCT AGC^GCT
EcoNI 632 CCTTCCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 413 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRI 520 GAATTC G^AATTC
EcoT14I 368 CCTTGG C^C[AT][AT]GG
FokI 240 GGATG GGATG(9/13)
KasI 21 GGCGCC G^GCGCC
Ksp632I 198 CTCTTC CTCTTC(1/4)
MflI 387 AGATCC [GA]^GATC[CT]
MroI 635 TCCGGA T^CCGGA
NaeI 18 GCCGGC GCC^GGC
NarI 21 GGCGCC GG^CGCC
NgoMIV 18 GCCGGC G^CCGGC
PleI 732 GACTC GAGTC(4/5)
PvuII 253 CAGCTG CAG^CTG
SacI 772 GAGCTC GAGCT^C
SapI 197 GCTCTTC GCTCTTC(1/4)
SbfI 427 CCTGCAGG CCTGCA^GG
SfoI 21 GGCGCC GGC^GCC
SgrAI 23 CGCCGGTG C[GA]^CCGG[CT]G
SmlI 331 CTCAAG C^T[CT][GA]AG
Sse8387I 427 CCTGCAGG CCTGCA^GG
StyI 368 CCTTGG C^C[AT][AT]GG
TaqI 452 TCGA T^CGA
TfiI 716 GATTC G^A[AT]TC
Tsp509I 521 AATT ^AATT
TthHB8I 452 TCGA T^CGA
XcmI 359 CCAACCTGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 387 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 21 GGCGCC G[GA]^CG[CT]C
494 GACGTC
AspHI 148 GTGCAC G[AT]GC[AT]^C
772 GAGCTC
BanII 414 GGGCCC G[GA]GC[CT]^C
772 GAGCTC
BcnI 177 CCCGG CC^[GC]GG
217 CCGGG
BpmI 470 CTCCAG CTGGAG(16/14)
311 CTGGAG
BsaHI 21 GGCGCC G[GA]^CG[CT]C
494 GACGTC
BsiHKAI 148 GTGCAC G[AT]GC[AT]^C
772 GAGCTC
BsmAI 656 GAGAC GTCTC(1/5)
468 GTCTC
BsrBI 580 GAGCGG CCGCTC(-3/-3)
598 GAGCGG
BsrFI 18 GCCGGC [GA]^CCGG[CT]
24 GCCGGT
Cfr10I 18 GCCGGC [GA]^CCGG[CT]
24 GCCGGT
FauI 259 CCCGC CCCGC(4/6)
407 GCGGG
HaeII 21 GGCGCC [GA]GCGC^[CT]
327 AGCGCT
Hin1I 21 GGCGCC G[GA]^CG[CT]C
494 GACGTC
HinfI 716 GATTC G^A[ACGT]TC
732 GACTC
MaeII 455 ACGT A^CGT
495 ACGT
MaeIII 457 GTGAC ^GT[ACGT]AC
614 GTGAC
MboII 385 GAAGA GAAGA(8/7)
199 TCTTC
MspA1I 253 CAGCTG C[AC]G^C[GT]G
308 CCGCTG
NciI 177 CCCGG CC^[GC]GG
217 CCGGG
PstI 46 CTGCAG CTGCA^G
428 CTGCAG
SfaNI 241 GATGC GCATC(5/9)
156 GCATC
SfcI 46 CTGCAG C^T[GA][CT]AG
428 CTGCAG
Tsp45I 457 GTGAC ^GT[GC]AC
614 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AccII AciI AluI Asp700I BanI
BbvI BmyI BsaJI BsiYI BslI
Bsp1286I BspMI BssKI BstNI BstUI
Cac8I CfoI Cfr13I DpnII EaeI
EcoRII Fnu4HI HaeIII HapII HgaI
HhaI HinP1I HpaII HphI ItaI
MboI MnlI MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI TseI TspRI XmnI