Restriction map for
pFN21AE1859
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# NO CUT ENZYMES ( 141 enzymes) #
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AatII Acc65I AccI AccII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaI
BsgI BsiHKAI BsiWI BsmAI BsmBI
BsmI BspDI BspHI BspLU11I BspMI
BsrBI BsrDI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII ClaI CpoI
DraII DraIII DrdI Eam1105I Eco47III
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoT22I FbaI FseI HaeII HincII
HindII HindIII HpaI KasI KpnI
KspI MaeII MfeI MluI MluNI
MscI MslI MspA1I MunI MvnI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SphI Sse8387I StuI SwaI
TfiI Tth111I Van91I XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccIII 407 TCCGGA T^CCGGA
AcyI 141 GGCGTC G[GA]^CG[CT]C
Asp700I 991 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 30 TGCGCA TGC^GCA
BanI 231 GGTGCC G^G[CT][GA]CC
BanII 43 GAGCCC G[GA]GC[CT]^C
BmyI 43 GAGCCC G[AGT]GC[ACT]^C
BsaBI 220 GATATAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 141 GGCGTC G[GA]^CG[CT]C
BseAI 407 TCCGGA T^CCGGA
BseRI 478 GAGGAG GAGGAG(10/8)
BsiEI 119 CGGCCG CG[GA][CT]^CG
Bsp1286I 43 GAGCCC G[AGT]GC[ACT]^C
BspEI 407 TCCGGA T^CCGGA
BsrFI 54 GCCGGC [GA]^CCGG[CT]
BsrI 742 ACTGG ACTGG(1/-1)
BstXI 251 CCAATCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 54 GCCGGC [GA]^CCGG[CT]
DraI 637 TTTAAA TTT^AAA
EagI 119 CGGCCG C^GGCCG
EarI 109 GAAGAG CTCTTC(1/4)
EclXI 119 CGGCCG C^GGCCG
Eco52I 119 CGGCCG C^GGCCG
EcoRV 949 GATATC GAT^ATC
EcoT14I 149 CCATGG C^C[AT][AT]GG
FauI 66 CCCGC CCCGC(4/6)
FspI 30 TGCGCA TGC^GCA
HgaI 142 GCGTC GACGC(5/10)
Hin1I 141 GGCGTC G[GA]^CG[CT]C
HphI 1117 TCACC GGTGA(8/7)
Ksp632I 109 GAAGAG CTCTTC(1/4)
MamI 220 GATATAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 407 TCCGGA T^CCGGA
NaeI 54 GCCGGC GCC^GGC
NcoI 149 CCATGG C^CATGG
NgoMIV 54 GCCGGC G^CCGGC
NspI 962 ACATGC [GA]CATG^[CT]
SpeI 804 ACTAGT A^CTAGT
SspI 1061 AATATT AAT^ATT
StyI 149 CCATGG C^C[AT][AT]GG
TaqI 615 TCGA T^CGA
TthHB8I 615 TCGA T^CGA
XmnI 991 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AvaI 46 CCCGGG C^[CT]CG[GA]G
359 CCCGGG
AvaII 261 GGTCC G^G[AT]CC
669 GGACC
BbsI 958 GAAGAC GAAGAC(2/6)
991 GAAGAC
BpuAI 958 GAAGAC GAAGAC(2/6)
991 GAAGAC
BsaWI 228 TCCGGT [AT]^CCGG[AT]
407 TCCGGA
BsmFI 200 GGGAC GGGAC(10/14)
144 GTCCC
BsoBI 46 CCCGGG C^[CT]CG[GA]G
359 CCCGGG
Bsp1407I 194 TGTACA T^GTACA
1128 TGTACA
BsrGI 194 TGTACA T^GTACA
1128 TGTACA
BstNI 171 CCTGG CC^[AT]GG
605 CCTGG
Cac8I 41 GCGAGC GC[ACGT]^[ACGT]GC
54 GCCGGC
CfoI 31 GCGC GCG^C
58 GCGC
DdeI 535 CTAAG C^T[ACGT]AG
548 CTCAG
EaeI 5 CGGCCA [CT]^GGCC[GA]
119 CGGCCG
EcoRII 171 CCTGG ^CC[AT]GG
605 CCTGG
HhaI 31 GCGC GCG^C
58 GCGC
HinP1I 31 GCGC G^CGC
58 GCGC
HinfI 175 GACTC G^A[ACGT]TC
1114 GACTC
MvaI 171 CCTGG CC^[AT]GG
605 CCTGG
PleI 175 GACTC GAGTC(4/5)
1114 GACTC
PstI 319 CTGCAG CTGCA^G
658 CTGCAG
SfcI 319 CTGCAG C^T[GA][CT]AG
658 CTGCAG
SmaI 46 CCCGGG CCC^GGG
359 CCCGGG
SspBI 194 TGTACA T^GTACA
1128 TGTACA
XmaCI 46 CCCGGG C^CCGGG
359 CCCGGG
XmaI 46 CCCGGG C^CCGGG
359 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BbvI BcnI BfaI BsaJI
BsiYI BslI BssKI BstF5I BstYI
Cfr13I DpnII DsaI Fnu4HI FokI
HaeIII HapII HpaII ItaI MaeI
MaeIII MboI MboII MflI MnlI
MseI MspI MwoI NciI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII