Restriction map for
pFN21AE1726
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaBI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HindIII HpaI HphI KasI KpnI
KspI MaeI MamI MfeI MluI
MroI MslI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PmaCI PmeI PmlI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TfiI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AcyI 416 GACGCC G[GA]^CG[CT]C
AfaI 48 GTAC GT^AC
AgeI 512 ACCGGT A^CCGGT
AseI 600 ATTAAT AT^TAAT
AsnI 600 ATTAAT AT^TAAT
BamHI 175 GGATCC G^GATCC
BanII 295 GAGCCC G[GA]GC[CT]^C
BbsI 367 GAAGAC GAAGAC(2/6)
BcnI 58 CCGGG CC^[GC]GG
BglI 11 GCCCCGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 295 GAGCCC G[AGT]GC[ACT]^C
BpmI 446 CTGGAG CTGGAG(16/14)
BpuAI 367 GAAGAC GAAGAC(2/6)
BsaAI 49 TACGTG [CT]AC^GT[GA]
BsaHI 416 GACGCC G[GA]^CG[CT]C
BsaI 345 GAGACC GGTCTC(1/5)
BsaWI 512 ACCGGT [AT]^CCGG[AT]
Bsp1286I 295 GAGCCC G[AGT]GC[ACT]^C
BspMI 393 GCAGGT ACCTGC(4/8)
BsrBI 540 CCGCTC CCGCTC(-3/-3)
BstYI 175 GGATCC [GA]^GATC[CT]
DpnII 176 GATC ^GATC
DsaI 375 CCGTGG C^C[GA][CT]GG
HaeII 134 GGCGCT [GA]GCGC^[CT]
Hin1I 416 GACGCC G[GA]^CG[CT]C
HincII 553 GTTGAC GT[CT]^[GA]AC
HindII 553 GTTGAC GT[CT]^[GA]AC
MaeII 50 ACGT A^CGT
MaeIII 562 GTGAC ^GT[ACGT]AC
MboI 176 GATC ^GATC
MflI 175 GGATCC [GA]^GATC[CT]
NaeI 121 GCCGGC GCC^GGC
NciI 58 CCGGG CC^[GC]GG
NdeII 176 GATC ^GATC
NgoMIV 121 GCCGGC G^CCGGC
PinAI 512 ACCGGT A^CCGGT
PpuMI 256 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 564 GACTGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 600 ATTAAT AT^TAAT
PvuII 665 CAGCTG CAG^CTG
RsaI 48 GTAC GT^AC
Sau3AI 176 GATC ^GATC
SfaNI 193 GCATC GCATC(5/9)
SgrAI 511 CACCGGTG C[GA]^CCGG[CT]G
TaqI 314 TCGA T^CGA
Tsp45I 562 GTGAC ^GT[GC]AC
Tsp509I 603 AATT ^AATT
TthHB8I 314 TCGA T^CGA
XhoII 175 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 5 CGCG CG^CG
73 CGCG
AlwI 176 GATCC GGATC(4/5)
175 GGATC
BalI 18 TGGCCA TGG^CCA
378 TGGCCA
BseRI 615 GAGGAG GAGGAG(10/8)
631 GAGGAG
BsmAI 199 GAGAC GTCTC(1/5)
345 GAGAC
BsrFI 121 GCCGGC [GA]^CCGG[CT]
512 ACCGGT
BsrI 489 ACTGG ACTGG(1/-1)
310 CCAGT
BstUI 5 CGCG CG^CG
73 CGCG
CfoI 6 GCGC GCG^C
135 GCGC
Cfr10I 121 GCCGGC [GA]^CCGG[CT]
512 ACCGGT
DraII 225 GGGGCCT [GA]G^G[ACGT]CC[CT]
256 AGGTCCC
EaeI 18 TGGCCA [CT]^GGCC[GA]
378 TGGCCA
EarI 474 GAAGAG CTCTTC(1/4)
646 GAAGAG
EcoO109I 225 GGGGCCT [GA]G^G[ACGT]CC[CT]
256 AGGTCCC
HhaI 6 GCGC GCG^C
135 GCGC
HinP1I 6 GCGC G^CGC
135 GCGC
Ksp632I 474 GAAGAG CTCTTC(1/4)
646 GAAGAG
MluNI 18 TGGCCA TGG^CCA
378 TGGCCA
MscI 18 TGGCCA TGG^CCA
378 TGGCCA
MseI 593 TTAA T^TAA
601 TTAA
MspA1I 14 CCGCTG C[AC]G^C[GT]G
665 CAGCTG
MvnI 5 CGCG CG^CG
73 CGCG
NlaIII 549 CATG CATG^
559 CATG
Tru9I 593 TTAA T^TAA
601 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AluI AvaII BbvI BsaJI
BsiYI BslI BsmFI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI EcoRII
FauI Fnu4HI FokI HaeIII HapII
HgaI HinfI HpaII ItaI MboII
MnlI MspI MvaI MwoI NlaIV
PleI PstI Sau96I ScrFI SfcI
TseI TspRI