Restriction map for
pFN21AE1240
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccII AclI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspHI AspI AvaI AvaII AvrII
BalI BamHI BanII BbrPI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BsaAI BsaBI BsaI BsaJI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1286I Bsp1407I BspDI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
Cfr10I Cfr13I ClaI CpoI DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
HpaI KpnI KspI MamI MluI
MluNI MscI MvnI NaeI NciI
NcoI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PstI PvuI RsrII
SacI SacII SalI SapI Sau96I
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI StuI StyI
SwaI TaqI Tth111I TthHB8I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccI 559 GTCTAC GT^[AC][GT]AC
AccIII 56 TCCGGA T^CCGGA
AcyI 31 GGCGCC G[GA]^CG[CT]C
AflIII 771 ACGTGT A^C[GA][CT]GT
AlwNI 1273 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 646 ATTAAT AT^TAAT
AsnI 646 ATTAAT AT^TAAT
Asp700I 700 GAAGTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 23 TGCGCA TGC^GCA
BanI 31 GGCGCC G^G[CT][GA]CC
BbeI 31 GGCGCC GGCGC^C
BbsI 801 GAAGAC GAAGAC(2/6)
BfaI 1081 CTAG C^TAG
BpmI 1169 CTGGAG CTGGAG(16/14)
BpuAI 801 GAAGAC GAAGAC(2/6)
BsaHI 31 GGCGCC G[GA]^CG[CT]C
BsaWI 56 TCCGGA [AT]^CCGG[AT]
BseAI 56 TCCGGA T^CCGGA
BsmFI 1149 GGGAC GGGAC(10/14)
BspEI 56 TCCGGA T^CCGGA
BspMI 448 GCAGGT ACCTGC(4/8)
BssKI 1175 CCAGG ^CC[ACGT]GG
BstNI 1175 CCAGG CC^[AT]GG
BstXI 395 CCATCATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 157 GGATCT [GA]^GATC[CT]
EarI 903 GAAGAG CTCTTC(1/4)
EcoRII 1175 CCAGG ^CC[AT]GG
FauI 35 CCCGC CCCGC(4/6)
FspI 23 TGCGCA TGC^GCA
HaeII 31 GGCGCC [GA]GCGC^[CT]
HaeIII 405 GGCC GG^CC
HapII 57 CCGG C^CGG
Hin1I 31 GGCGCC G[GA]^CG[CT]C
HincII 731 GTCAAC GT[CT]^[GA]AC
HindII 731 GTCAAC GT[CT]^[GA]AC
HpaII 57 CCGG C^CGG
KasI 31 GGCGCC G^GCGCC
Ksp632I 903 GAAGAG CTCTTC(1/4)
MaeI 1081 CTAG C^TAG
MaeII 771 ACGT A^CGT
MflI 157 GGATCT [GA]^GATC[CT]
MroI 56 TCCGGA T^CCGGA
MspI 57 CCGG C^CGG
MvaI 1175 CCAGG CC^[AT]GG
NarI 31 GGCGCC GG^CGCC
NspI 464 GCATGT [GA]CATG^[CT]
PflMI 505 CCATATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1194 GACTC GAGTC(4/5)
PshBI 646 ATTAAT AT^TAAT
ScrFI 1175 CCAGG CC^[ACGT]GG
SfcI 956 CTGTAG C^T[GA][CT]AG
SfoI 31 GGCGCC GGC^GCC
SspI 613 AATATT AAT^ATT
TfiI 240 GATTC G^A[AT]TC
Tsp45I 712 GTCAC ^GT[GC]AC
Van91I 505 CCATATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 813 CCAGAAAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 157 GGATCT [GA]^GATC[CT]
XmnI 700 GAAGTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcsI 569 AAATTC [GA]^AATT[CT]
688 AAATTT
ApoI 569 AAATTC [GA]^AATT[CT]
688 AAATTT
BsgI 67 GTGCAG GTGCAG(16/14)
329 GTGCAG
BspHI 398 TCATGA T^CATGA
884 TCATGA
CfoI 24 GCGC GCG^C
32 GCGC
DdeI 194 CTAAG C^T[ACGT]AG
590 CTGAG
HgaI 634 GACGC GACGC(5/10)
949 GACGC
HhaI 24 GCGC GCG^C
32 GCGC
HinP1I 24 GCGC G^CGC
32 GCGC
HindIII 196 AAGCTT A^AGCTT
520 AAGCTT
HinfI 240 GATTC G^A[ACGT]TC
1194 GACTC
HphI 292 GGTGA GGTGA(8/7)
747 GGTGA
MfeI 977 CAATTG C^AATTG
1157 CAATTG
MunI 977 CAATTG C^AATTG
1157 CAATTG
RcaI 398 TCATGA T^CATGA
884 TCATGA
SfaNI 895 GATGC GCATC(5/9)
997 GCATC
TspRI 854 CACTG CA[GC]TG[ACGT][ACGT]^
923 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BseRI BsiYI BslI BsrDI BsrI
BstF5I Cac8I DpnII Fnu4HI FokI
ItaI MaeIII MboI MboII MnlI
MseI MslI MspA1I MwoI NdeI
NdeII NlaIII NlaIV PvuII RsaI
Sau3AI Tru9I TseI Tsp509I