Restriction map for
pFN21AE2163
##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII AccI AclI AflII AgeI
AocI ApaI AscI AseI AsnI
AspI AvrII BamHI BcgI BciVI
BclI BfrI BglI BlnI BlpI
Bpu1102I BsaBI BsiEI BsiWI BsmBI
BspDI BsrDI BssHII BssSI Bst1107I
BstBI BstZ17I Bsu36I CelII ClaI
DraI EagI EclXI Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI HpaI MamI MfeI MluI
MunI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshBI Psp1406I
PvuI SalI SbfI SfiI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspI SwaI Tth111I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccIII 20 TCCGGA T^CCGGA
AcsI 1081 AAATTC [GA]^AATT[CT]
AhdI 1777 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1315 GTGCAC G^TGCAC
Aor51HI 2285 AGCGCT AGC^GCT
ApaLI 1315 GTGCAC G^TGCAC
ApoI 1081 AAATTC [GA]^AATT[CT]
AspEI 1777 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 537 TGCGCA TGC^GCA
BbvCI 1105 GCTGAGG CCTCAGC(-5/-2)
BfaI 635 CTAG C^TAG
BglII 1715 AGATCT A^GATCT
BsaI 1912 GAGACC GGTCTC(1/5)
BsaWI 20 TCCGGA [AT]^CCGG[AT]
BseAI 20 TCCGGA T^CCGGA
BsmAI 1912 GAGAC GTCTC(1/5)
BsmI 1259 GCATTC GAATGC(1/-1)
Bsp1407I 1619 TGTACA T^GTACA
BspEI 20 TCCGGA T^CCGGA
BspHI 1577 TCATGA T^CATGA
BspLU11I 1166 ACATGT A^CATGT
BsrBI 1738 CCGCTC CCGCTC(-3/-3)
BsrGI 1619 TGTACA T^GTACA
BstEII 2391 GGTGACC G^GT[ACGT]ACC
BstPI 2391 GGTGACC G^GT[ACGT]ACC
CpoI 2120 CGGACCG CG^G[AT]CCG
DraIII 1633 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1777 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2285 AGCGCT AGC^GCT
EcoO65I 2391 GGTGACC G^GT[ACGT]ACC
FspI 537 TGCGCA TGC^GCA
HincII 433 GTCAAC GT[CT]^[GA]AC
HindII 433 GTCAAC GT[CT]^[GA]AC
HindIII 871 AAGCTT A^AGCTT
KspI 2117 CCGCGG CCGC^GG
MaeI 635 CTAG C^TAG
MroI 20 TCCGGA T^CCGGA
MseI 1937 TTAA T^TAA
PshAI 1762 GACAAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1324 CAGCTG CAG^CTG
RcaI 1577 TCATGA T^CATGA
RsrII 2120 CGGACCG CG^G[AT]CCG
SacII 2117 CCGCGG CCGC^GG
SapI 1539 GCTCTTC GCTCTTC(1/4)
SexAI 1634 ACCTGGT A^CC[AT]GGT
SspBI 1619 TGTACA T^GTACA
StuI 129 AGGCCT AGG^CCT
Tru9I 1937 TTAA T^TAA
Tsp509I 1082 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 1096 GGTACC G^GTACC
2138 GGTACC
AlwI 1926 GATCC GGATC(4/5)
2431 GATCC
Asp718I 1096 GGTACC G^GTACC
2138 GGTACC
BbeI 1287 GGCGCC GGCGC^C
2055 GGCGCC
BbrPI 37 CACGTG CAC^GTG
1318 CACGTG
BbsI 1794 GAAGAC GAAGAC(2/6)
877 GTCTTC
BpuAI 1794 GAAGAC GAAGAC(2/6)
877 GTCTTC
BsrFI 766 GCCGGC [GA]^CCGG[CT]
2206 GCCGGC
BstAPI 1632 GCACCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1674 GCAGACACTGC
BstYI 1715 AGATCT [GA]^GATC[CT]
1925 AGATCC
Cfr10I 766 GCCGGC [GA]^CCGG[CT]
2206 GCCGGC
DrdI 726 GACGCGCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1581 GACGCACCTGTC
EarI 1462 CTCTTC CTCTTC(1/4)
1540 CTCTTC
KasI 1287 GGCGCC G^GCGCC
2055 GGCGCC
KpnI 1096 GGTACC GGTAC^C
2138 GGTACC
Ksp632I 1462 CTCTTC CTCTTC(1/4)
1540 CTCTTC
MflI 1715 AGATCT [GA]^GATC[CT]
1925 AGATCC
NaeI 766 GCCGGC GCC^GGC
2206 GCCGGC
NarI 1287 GGCGCC GG^CGCC
2055 GGCGCC
NgoMIV 766 GCCGGC G^CCGGC
2206 GCCGGC
PleI 313 GAGTC GAGTC(4/5)
739 GAGTC
PmaCI 37 CACGTG CAC^GTG
1318 CACGTG
PmlI 37 CACGTG CAC^GTG
1318 CACGTG
PstI 591 CTGCAG CTGCA^G
1672 CTGCAG
SacI 1537 GAGCTC GAGCT^C
2572 GAGCTC
ScaI 470 AGTACT AGT^ACT
1706 AGTACT
SfcI 591 CTGCAG C^T[GA][CT]AG
1672 CTGCAG
SfoI 1287 GGCGCC GGC^GCC
2055 GGCGCC
XhoII 1715 AGATCT [GA]^GATC[CT]
1925 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwNI Asp700I AspHI AvaI
AvaII BalI BanI BanII BbvI
BcnI BmyI BpmI BsaAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
BstXI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI SphI StyI TaqI
TfiI TseI Tsp45I TspRI TthHB8I
Van91I XcmI XmnI