Restriction map for
pFN21AE2109
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# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AgeI Alw44I ApaLI AscI AseI
AsnI Asp718I AspI BalI BamHI
BbeI BbrPI BbsI BbvCI BcgI
BclI BglII BlpI Bpu1102I BpuAI
BsaAI BsaBI BseAI BsiWI BsmBI
BspDI BspEI BspHI BspLU11I BssHII
Bst1107I BstBI BstZ17I CelII ClaI
CpoI EcoRI EcoRV EcoT22I FbaI
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspI SwaI Tth111I XbaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AcsI 1889 AAATTC [GA]^AATT[CT]
AcyI 621 GGCGTC G[GA]^CG[CT]C
AflII 249 CTTAAG C^TTAAG
AocI 188 CCTCAGG CC^T[ACGT]AGG
Aor51HI 903 AGCGCT AGC^GCT
ApoI 1889 AAATTC [GA]^AATT[CT]
Asp700I 1815 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1395 TGCGCA TGC^GCA
AvrII 58 CCTAGG C^CTAGG
BciVI 2459 GTATCC GTATCC(6/5)
BfrI 249 CTTAAG C^TTAAG
BlnI 58 CCTAGG C^CTAGG
BsaHI 621 GGCGTC G[GA]^CG[CT]C
BsaI 510 GGTCTC GGTCTC(1/5)
BsaWI 32 ACCGGA [AT]^CCGG[AT]
BsiEI 1271 CGGCCG CG[GA][CT]^CG
Bsp1407I 2226 TGTACA T^GTACA
BspMI 2012 ACCTGC ACCTGC(4/8)
BsrGI 2226 TGTACA T^GTACA
Bsu36I 188 CCTCAGG CC^T[ACGT]AGG
DraI 333 TTTAAA TTT^AAA
DraIII 1228 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1271 CGGCCG C^GGCCG
EclXI 1271 CGGCCG C^GGCCG
Eco47III 903 AGCGCT AGC^GCT
Eco52I 1271 CGGCCG C^GGCCG
Eco81I 188 CCTCAGG CC^T[ACGT]AGG
FseI 1272 GGCCGGCC GGCCGG^CC
FspI 1395 TGCGCA TGC^GCA
Hin1I 621 GGCGTC G[GA]^CG[CT]C
HincII 1222 GTCAAC GT[CT]^[GA]AC
HindII 1222 GTCAAC GT[CT]^[GA]AC
PaeR7I 2679 CTCGAG C^TCGAG
PshAI 2749 GACCGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfiI 1246 GGCCTGCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 2226 TGTACA T^GTACA
XhoI 2679 CTCGAG C^TCGAG
XmnI 1815 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AhdI 630 GACCTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
642 GACGGAAAGTC
AspEI 630 GACCTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
642 GACGGAAAGTC
BanI 1092 GGTGCC G^G[CT][GA]CC
2559 GGTGCC
BsgI 1237 GTGCAG GTGCAG(16/14)
2035 GTGCAG
BsmI 558 GAATGC GAATGC(1/-1)
1073 GCATTC
BsrBI 888 CCGCTC CCGCTC(-3/-3)
1342 CCGCTC
BsrDI 1498 CATTGC GCAATG(2/0)
553 GCAATG
BssSI 1174 CACGAG CACGAG(-5/-1)
1549 CTCGTG
BstEII 297 GGTCACC G^GT[ACGT]ACC
450 GGTCACC
BstPI 297 GGTCACC G^GT[ACGT]ACC
450 GGTCACC
BstXI 971 CCACGTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2463 CCATGTCCATGG
BstYI 747 GGATCT [GA]^GATC[CT]
1754 AGATCC
DrdI 771 GACATCCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2575 GACTGCCTAGTC
Eam1105I 630 GACCTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
642 GACGGAAAGTC
EarI 2439 CTCTTC CTCTTC(1/4)
897 GAAGAG
EcoO65I 297 GGTCACC G^GT[ACGT]ACC
450 GGTCACC
Ksp632I 2439 CTCTTC CTCTTC(1/4)
897 GAAGAG
MflI 747 GGATCT [GA]^GATC[CT]
1754 AGATCC
MseI 250 TTAA T^TAA
334 TTAA
NcoI 2469 CCATGG C^CATGG
2594 CCATGG
PpuMI 595 AGGTCCC [GA]G^G[AT]CC[CT]
874 AGGTCCC
SexAI 592 ACCAGGT A^CC[AT]GGT
871 ACCAGGT
SphI 520 GCATGC GCATG^C
1181 GCATGC
TfiI 290 GATTC G^A[AT]TC
2518 GATTC
Tru9I 250 TTAA T^TAA
334 TTAA
XcmI 971 CCACGTCCTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2469 CCATGGATTTTATGG
XhoII 747 GGATCT [GA]^GATC[CT]
1754 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccI AccII AciI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BanII BbvI BcnI BfaI
BglI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StuI StyI TaqI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XmaCI XmaI