Restriction map for
pFN21AE1957
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# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AvrII BalI BamHI BbrPI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BsaAI BsaBI BsgI
BsiWI BsmI BspDI BspHI BsrBI
BsrDI Bst1107I BstBI BstZ17I ClaI
CpoI DraI Eam1105I Eco47III EcoRI
FbaI FseI KpnI MaeI MamI
MfeI MluI MluNI MscI MunI
NheI NotI NruI NspV PacI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI TfiI Van91I XbaI XcmI
XmaCI XmaI
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# ONE CUT ENZYMES ( 73 enzymes) #
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AccIII 5 TCCGGA T^CCGGA
AcsI 429 AAATTT [GA]^AATT[CT]
AocI 1171 CCTCAGG CC^T[ACGT]AGG
ApoI 429 AAATTT [GA]^AATT[CT]
Asp700I 1243 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1362 GAGCAC G[AT]GC[AT]^C
AspI 1032 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 595 CTCGAG C^[CT]CG[GA]G
AviII 698 TGCGCA TGC^GCA
BbeI 196 GGCGCC GGCGC^C
BbsI 1282 GTCTTC GAAGAC(2/6)
BbvCI 608 CCTCAGC CCTCAGC(-5/-2)
BglI 647 GCCTCATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 108 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 108 GCTCAGC GC^T[ACGT]AGC
BpuAI 1282 GTCTTC GAAGAC(2/6)
BsaI 184 GAGACC GGTCTC(1/5)
BsaWI 5 TCCGGA [AT]^CCGG[AT]
BseAI 5 TCCGGA T^CCGGA
BsiEI 57 CGGCCG CG[GA][CT]^CG
BsiHKAI 1362 GAGCAC G[AT]GC[AT]^C
BsoBI 595 CTCGAG C^[CT]CG[GA]G
Bsp1407I 794 TGTACA T^GTACA
BspEI 5 TCCGGA T^CCGGA
BspLU11I 791 ACATGT A^CATGT
BsrGI 794 TGTACA T^GTACA
BstAPI 1364 GCACATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1422 GGTGACC G^GT[ACGT]ACC
BstPI 1422 GGTGACC G^GT[ACGT]ACC
BstXI 299 CCAAAACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1120 GGATCT [GA]^GATC[CT]
Bsu36I 1171 CCTCAGG CC^T[ACGT]AGG
CelII 108 GCTCAGC GC^T[ACGT]AGC
DrdI 9 GACCCCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 57 CGGCCG C^GGCCG
EclXI 57 CGGCCG C^GGCCG
Eco52I 57 CGGCCG C^GGCCG
Eco81I 1171 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1422 GGTGACC G^GT[ACGT]ACC
EcoRV 991 GATATC GAT^ATC
EcoT22I 881 ATGCAT ATGCA^T
FspI 698 TGCGCA TGC^GCA
HgaI 129 GCGTC GACGC(5/10)
HincII 418 GTTAAC GT[CT]^[GA]AC
HindII 418 GTTAAC GT[CT]^[GA]AC
HindIII 819 AAGCTT A^AGCTT
HinfI 1224 GAGTC G^A[ACGT]TC
HpaI 418 GTTAAC GTT^AAC
KasI 196 GGCGCC G^GCGCC
KspI 54 CCGCGG CCGC^GG
MflI 1120 GGATCT [GA]^GATC[CT]
MroI 5 TCCGGA T^CCGGA
MseI 419 TTAA T^TAA
NaeI 113 GCCGGC GCC^GGC
NarI 196 GGCGCC GG^CGCC
NdeI 1349 CATATG CA^TATG
NgoMIV 113 GCCGGC G^CCGGC
NsiI 881 ATGCAT ATGCA^T
PaeR7I 595 CTCGAG C^TCGAG
PflFI 1032 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1224 GAGTC GAGTC(4/5)
SacII 54 CCGCGG CCGC^GG
SapI 1243 GAAGAGC GCTCTTC(1/4)
SfoI 196 GGCGCC GGC^GCC
SmlI 595 CTCGAG C^T[CT][GA]AG
SspBI 794 TGTACA T^GTACA
TaqI 596 TCGA T^CGA
Tru9I 419 TTAA T^TAA
Tth111I 1032 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 596 TCGA T^CGA
XhoI 595 CTCGAG C^TCGAG
XhoII 1120 GGATCT [GA]^GATC[CT]
XmnI 1243 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 128 GGCGTC G[GA]^CG[CT]C
196 GGCGCC
AfaI 752 GTAC GT^AC
795 GTAC
AflIII 791 ACATGT A^C[GA][CT]GT
1073 ACGTGT
AlwI 3 GATCC GGATC(4/5)
1120 GGATC
BcnI 193 CCGGG CC^[GC]GG
247 CCGGG
BsaHI 128 GGCGTC G[GA]^CG[CT]C
196 GGCGCC
BsmBI 504 CGTCTC CGTCTC(1/5)
467 GAGACG
BsrFI 113 GCCGGC [GA]^CCGG[CT]
1163 GCCGGT
BssHII 104 GCGCGC G^CGCGC
323 GCGCGC
Cfr10I 113 GCCGGC [GA]^CCGG[CT]
1163 GCCGGT
DraIII 1381 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
1521 CACCCAGTG
EarI 235 CTCTTC CTCTTC(1/4)
1243 GAAGAG
EcoNI 372 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1206 CCTGATAGAGG
HaeII 196 GGCGCC [GA]GCGC^[CT]
259 AGCGCC
Hin1I 128 GGCGTC G[GA]^CG[CT]C
196 GGCGCC
Ksp632I 235 CTCTTC CTCTTC(1/4)
1243 GAAGAG
MaeII 803 ACGT A^CGT
1073 ACGT
NciI 193 CCGGG CC^[GC]GG
247 CCGGG
PvuII 611 CAGCTG CAG^CTG
947 CAGCTG
RsaI 752 GTAC GT^AC
795 GTAC
StuI 276 AGGCCT AGG^CCT
1488 AGGCCT
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# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccII AciI AluI AlwNI AvaII
BanI BanII BbvI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BssSI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NcoI NdeII NlaIII NlaIV NspI
PpuMI PstI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TseI Tsp45I
Tsp509I TspRI