Restriction map for
pFN21AE1183
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# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII AclI AcsI AflII AhdI
AocI Aor51HI ApoI AscI AseI
AsnI AspEI AvrII BamHI BbrPI
BbvCI BclI BfrI BglII BlnI
BsaBI BsiEI BsiWI BsmI BspDI
BspHI BsrDI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HindIII HpaI MamI MfeI
MluI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI SalI SapI
SbfI SexAI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tsp509I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 515 GGTACC G^GTACC
AgeI 512 ACCGGT A^CCGGT
Alw44I 1533 GTGCAC G^TGCAC
ApaLI 1533 GTGCAC G^TGCAC
Asp700I 912 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 515 GGTACC G^GTACC
AviII 1249 TGCGCA TGC^GCA
BalI 1943 TGGCCA TGG^CCA
BcgI 158 GCACCCTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 765 GGATAC GTATCC(6/5)
BfaI 451 CTAG C^TAG
BlpI 1677 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1677 GCTCAGC GC^T[ACGT]AGC
BsaAI 1006 TACGTG [CT]AC^GT[GA]
BsmBI 1243 CGTCTC CGTCTC(1/5)
Bsp1407I 14 TGTACA T^GTACA
BsrGI 14 TGTACA T^GTACA
BstAPI 300 GCACCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1677 GCTCAGC GC^T[ACGT]AGC
CpoI 213 CGGACCG CG^G[AT]CCG
EarI 250 GAAGAG CTCTTC(1/4)
EcoNI 882 CCTTTTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 1249 TGCGCA TGC^GCA
KpnI 515 GGTACC GGTAC^C
Ksp632I 250 GAAGAG CTCTTC(1/4)
KspI 2056 CCGCGG CCGC^GG
MaeI 451 CTAG C^TAG
MluNI 1943 TGGCCA TGG^CCA
MscI 1943 TGGCCA TGG^CCA
MslI 1851 CACCAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 2009 GCCGGC GCC^GGC
NcoI 761 CCATGG C^CATGG
NgoMIV 2009 GCCGGC G^CCGGC
PinAI 512 ACCGGT A^CCGGT
PpuMI 1221 GGGTCCC [GA]G^G[AT]CC[CT]
RsrII 213 CGGACCG CG^G[AT]CCG
SacI 85 GAGCTC GAGCT^C
SacII 2056 CCGCGG CCGC^GG
ScaI 974 AGTACT AGT^ACT
SfiI 1518 GGCCATCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 14 TGTACA T^GTACA
Tsp45I 382 GTCAC ^GT[GC]AC
XmnI 912 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 34 GTCTAC GT^[AC][GT]AC
454 GTCTAC
AccIII 71 TCCGGA T^CCGGA
267 TCCGGA
AlwNI 634 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
2044 CAGGCCCTG
ApaI 674 GGGCCC GGGCC^C
829 GGGCCC
AspI 28 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
399 GACCGAGTC
AvaI 286 CCCGAG C^[CT]CG[GA]G
737 CCCGAG
BbeI 1143 GGCGCC GGCGC^C
1448 GGCGCC
BseAI 71 TCCGGA T^CCGGA
267 TCCGGA
BsoBI 286 CCCGAG C^[CT]CG[GA]G
737 CCCGAG
BspEI 71 TCCGGA T^CCGGA
267 TCCGGA
BspLU11I 11 ACATGT A^CATGT
983 ACATGT
BsrBI 651 CCGCTC CCGCTC(-3/-3)
424 GAGCGG
BstYI 1463 AGATCC [GA]^GATC[CT]
1964 AGATCC
DdeI 1040 CTGAG C^T[ACGT]AG
1678 CTCAG
HincII 1105 GTCAAC GT[CT]^[GA]AC
2080 GTCAAC
HindII 1105 GTCAAC GT[CT]^[GA]AC
2080 GTCAAC
KasI 1143 GGCGCC G^GCGCC
1448 GGCGCC
MflI 1463 AGATCC [GA]^GATC[CT]
1964 AGATCC
MroI 71 TCCGGA T^CCGGA
267 TCCGGA
MseI 541 TTAA T^TAA
1051 TTAA
NarI 1143 GGCGCC GG^CGCC
1448 GGCGCC
PflFI 28 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
399 GACCGAGTC
PflMI 633 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
756 CCACGCCATGG
PstI 684 CTGCAG CTGCA^G
840 CTGCAG
SfcI 684 CTGCAG C^T[GA][CT]AG
840 CTGCAG
SfoI 1143 GGCGCC GGC^GCC
1448 GGCGCC
SmlI 409 CTCAAG C^T[CT][GA]AG
1621 CTTGAG
Tru9I 541 TTAA T^TAA
1051 TTAA
TspRI 1207 CAGTG CA[GC]TG[ACGT][ACGT]^
1237 CAGTG
Tth111I 28 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
399 GACCGAGTC
Van91I 633 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
756 CCACGCCATGG
XcmI 101 CCAATGGGAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
803 CCATCTCGGCGCTGG
XhoII 1463 AGATCC [GA]^GATC[CT]
1964 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AspHI AvaII BanI
BanII BbsI BbvI BcnI BglI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI Cac8I CfoI
Cfr10I Cfr13I DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TfiI TseI TthHB8I