Restriction map for
pFN21AE1295
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# NO CUT ENZYMES ( 170 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AhdI
AlwI AlwNI AocI Aor51HI ApaI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BanI BbeI BbrPI BbvCI BbvI
BcgI BciVI BcnI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BseAI BseRI BsgI
BsiEI BsiWI BsmBI BsmFI BsoBI
Bsp1407I BspDI BspEI BspHI BspMI
BsrBI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I Cac8I CelII
CfoI ClaI CpoI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRV EcoT14I EcoT22I FauI
Fnu4HI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI ItaI KasI KpnI KspI
MaeII MamI MflI MluI MroI
MspA1I MvnI NaeI NarI NciI
NcoI NgoMIV NheI NlaIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfaNI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
StyI SwaI TseI Tth111I Van91I
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AciI 262 GCGG CCGC(-3/-1)
AflIII 517 ACATGT A^C[GA][CT]GT
AgeI 1376 ACCGGT A^CCGGT
Alw44I 277 GTGCAC G^TGCAC
ApaLI 277 GTGCAC G^TGCAC
Asp700I 414 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 277 GTGCAC G[AT]GC[AT]^C
BalI 160 TGGCCA TGG^CCA
BanII 85 GAGCCC G[GA]GC[CT]^C
BbsI 25 GTCTTC GAAGAC(2/6)
BclI 59 TGATCA T^GATCA
BglI 1201 GCCTTTAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 25 GTCTTC GAAGAC(2/6)
BsaJI 30 CCTGGG C^C[ACGT][ACGT]GG
BsaWI 1376 ACCGGT [AT]^CCGG[AT]
BsiHKAI 277 GTGCAC G[AT]GC[AT]^C
BsmI 1210 GCATTC GAATGC(1/-1)
BspLU11I 517 ACATGT A^CATGT
BsrFI 1376 ACCGGT [GA]^CCGG[CT]
BstAPI 270 GCATAAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 1376 ACCGGT [GA]^CCGG[CT]
DpnII 60 GATC ^GATC
DraI 1036 TTTAAA TTT^AAA
EaeI 160 TGGCCA [CT]^GGCC[GA]
FbaI 59 TGATCA T^GATCA
HaeIII 161 GGCC GG^CC
HapII 1377 CCGG C^CGG
HpaII 1377 CCGG C^CGG
HphI 1501 GGTGA GGTGA(8/7)
MboI 60 GATC ^GATC
MfeI 759 CAATTG C^AATTG
MluNI 160 TGGCCA TGG^CCA
MscI 160 TGGCCA TGG^CCA
MspI 1377 CCGG C^CGG
MunI 759 CAATTG C^AATTG
NdeI 1495 CATATG CA^TATG
NdeII 60 GATC ^GATC
PinAI 1376 ACCGGT A^CCGGT
Sau3AI 60 GATC ^GATC
SexAI 20 ACCTGGT A^CC[AT]GGT
SfcI 12 CTACAG C^T[GA][CT]AG
SspI 333 AATATT AAT^ATT
TaqI 1395 TCGA T^CGA
TthHB8I 1395 TCGA T^CGA
XbaI 1477 TCTAGA T^CTAGA
XmnI 414 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AluI 948 AGCT AG^CT
1368 AGCT
BmyI 85 GAGCCC G[AGT]GC[ACT]^C
277 GTGCAC
BsmAI 102 GAGAC GTCTC(1/5)
43 GTCTC
Bsp1286I 85 GAGCCC G[AGT]GC[ACT]^C
277 GTGCAC
BsrDI 848 GCAATG GCAATG(2/0)
1268 GCAATG
BssKI 21 CCTGG ^CC[ACGT]GG
30 CCTGG
BstF5I 571 CATCC GGATG(2/0)
264 GGATG
BstNI 21 CCTGG CC^[AT]GG
30 CCTGG
EarI 99 GAAGAG CTCTTC(1/4)
661 GAAGAG
EcoRI 902 GAATTC G^AATTC
1322 GAATTC
EcoRII 21 CCTGG ^CC[AT]GG
30 CCTGG
FokI 571 CATCC GGATG(9/13)
264 GGATG
HindIII 947 AAGCTT A^AGCTT
1367 AAGCTT
HinfI 181 GATTC G^A[ACGT]TC
582 GAATC
Ksp632I 99 GAAGAG CTCTTC(1/4)
661 GAAGAG
MaeIII 1184 GTGAC ^GT[ACGT]AC
1445 GTGAC
MslI 1091 CATACAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1495 CATATGGGTG
MvaI 21 CCTGG CC^[AT]GG
30 CCTGG
NspI 105 ACATGC [GA]CATG^[CT]
517 ACATGT
ScrFI 21 CCTGG CC^[ACGT]GG
30 CCTGG
TfiI 181 GATTC G^A[AT]TC
582 GAATC
Tsp45I 1184 GTGAC ^GT[GC]AC
1445 GTGAC
TspRI 1197 CAGTG CA[GC]TG[ACGT][ACGT]^
1449 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AcsI ApoI AvaII BfaI BpmI
BsiYI BslI BsrI Cfr13I DdeI
MaeI MboII MnlI MseI MwoI
NlaIII Sau96I Tru9I Tsp509I