Restriction map for
pFN21AA1007
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# NO CUT ENZYMES ( 65 enzymes) #
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AclI AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AspEI AvrII
BbeI BbrPI BglI BlnI BsaAI
BsiWI BspMI BsrBI BsrFI BssHII
Bst1107I BstZ17I Cfr10I CpoI EagI
Eam1105I EclXI Eco47III Eco52I FseI
HincII HindII HpaI KasI KspI
MluI NaeI NarI NgoMIV NotI
NruI PacI PaeR7I PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SgrAI SmaI SnaBI SpeI
Sse8387I SwaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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Acc65I 6809 GGTACC G^GTACC
AccI 1174 GTAGAC GT^[AC][GT]AC
AccII 5593 CGCG CG^CG
AccIII 727 TCCGGA T^CCGGA
AflIII 6443 ACATGT A^C[GA][CT]GT
Asp700I 896 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 6809 GGTACC G^GTACC
AvaI 4940 CTCGGG C^[CT]CG[GA]G
BseAI 727 TCCGGA T^CCGGA
BsiEI 7049 CGGTCG CG[GA][CT]^CG
BsmBI 1863 GAGACG CGTCTC(1/5)
BsoBI 4940 CTCGGG C^[CT]CG[GA]G
BspDI 4214 ATCGAT AT^CGAT
BspEI 727 TCCGGA T^CCGGA
BspLU11I 6443 ACATGT A^CATGT
BssSI 5821 CTCGTG CACGAG(-5/-1)
BstBI 4901 TTCGAA TT^CGAA
BstUI 5593 CGCG CG^CG
ClaI 4214 ATCGAT AT^CGAT
EcoRI 402 GAATTC G^AATTC
EcoRV 6730 GATATC GAT^ATC
FauI 4087 GCGGG CCCGC(4/6)
HaeII 6210 AGCGCC [GA]GCGC^[CT]
KpnI 6809 GGTACC GGTAC^C
MroI 727 TCCGGA T^CCGGA
MvnI 5593 CGCG CG^CG
NdeI 5084 CATATG CA^TATG
NheI 2098 GCTAGC G^CTAGC
NspV 4901 TTCGAA TT^CGAA
SacI 756 GAGCTC GAGCT^C
SexAI 44 ACCTGGT A^CC[AT]GGT
SfuI 4901 TTCGAA TT^CGAA
SphI 1617 GCATGC GCATG^C
SspI 6203 AATATT AAT^ATT
XbaI 2977 TCTAGA T^CTAGA
XmnI 896 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 33 enzymes) #
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AatII 490 GACGTC GACGT^C
2084 GACGTC
AcyI 490 GACGTC G[GA]^CG[CT]C
2084 GACGTC
AflII 619 CTTAAG C^TTAAG
4746 CTTAAG
AocI 121 CCTGAGG CC^T[ACGT]AGG
5526 CCTGAGG
AspI 267 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1865 GACGGTGTC
BamHI 713 GGATCC G^GATCC
2824 GGATCC
BanII 756 GAGCTC G[GA]GC[CT]^C
3253 GAGCCC
BbvCI 4406 CCTCAGC CCTCAGC(-5/-2)
1371 GCTGAGG
BclI 1656 TGATCA T^GATCA
3587 TGATCA
BfrI 619 CTTAAG C^TTAAG
4746 CTTAAG
BlpI 3784 GCTGAGC GC^T[ACGT]AGC
5728 GCTGAGC
Bpu1102I 3784 GCTGAGC GC^T[ACGT]AGC
5728 GCTGAGC
BsaHI 490 GACGTC G[GA]^CG[CT]C
2084 GACGTC
BsaI 3487 GAGACC GGTCTC(1/5)
5090 GGTCTC
BsaWI 727 TCCGGA [AT]^CCGG[AT]
6806 TCCGGT
BstAPI 1485 GCATACCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4155 GCAGTGTGTGC
BstEII 3102 GGTAACC G^GT[ACGT]ACC
3144 GGTCACC
BstPI 3102 GGTAACC G^GT[ACGT]ACC
3144 GGTCACC
BstXI 3748 CCAAACCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6934 CCACATCCTTGG
Bsu36I 121 CCTGAGG CC^T[ACGT]AGG
5526 CCTGAGG
CelII 3784 GCTGAGC GC^T[ACGT]AGC
5728 GCTGAGC
DraIII 1769 CACGTCGTG CAC[ACGT][ACGT][ACGT]^GTG
5115 CACAAGGTG
Eco81I 121 CCTGAGG CC^T[ACGT]AGG
5526 CCTGAGG
EcoNI 3888 CCTCCTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
6255 CCTCTACAAGG
EcoO65I 3102 GGTAACC G^GT[ACGT]ACC
3144 GGTCACC
FbaI 1656 TGATCA T^GATCA
3587 TGATCA
Hin1I 490 GACGTC G[GA]^CG[CT]C
2084 GACGTC
NcoI 2015 CCATGG C^CATGG
5095 CCATGG
PflFI 267 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1865 GACGGTGTC
PpuMI 536 AGGTCCT [GA]G^G[AT]CC[CT]
5879 AGGTCCT
PshAI 2969 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4744 GACTTAAGTC
Tth111I 267 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1865 GACGGTGTC
XcmI 558 CCATCTCCTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3745 CCACCAAACCCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI AspHI
AvaII AviII BalI BanI BbsI
BbvI BcgI BciVI BcnI BfaI
BglII BmyI BpmI BpuAI BsaBI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
Bsp1407I BspHI BsrDI BsrGI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DrdI DsaI EaeI EarI
EcoO109I EcoRII EcoT14I EcoT22I Fnu4HI
FokI FspI HaeIII HapII HgaI
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MfeI
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MunI MvaI
MwoI NciI NdeII NlaIII NlaIV
NsiI NspI PflMI PleI PshBI
PstI PvuII RcaI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII