Restriction map for
pFN21AE1504
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# NO CUT ENZYMES ( 88 enzymes) #
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Acc65I AccIII AflII AgeI AhdI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BamHI BbrPI
BciVI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaBI BseAI BsiWI
BsmI BspEI BspHI BssHII Bst1107I
BstEII BstPI BstZ17I CelII CpoI
EagI Eam1105I EarI EclXI Eco52I
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI Ksp632I KspI MamI MluI
MroI NaeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
PvuI RcaI RsrII SacII SalI
SapI ScaI SfiI SgrAI SmlI
SnaBI SpeI SspI StuI SwaI
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 1716 CGCG CG^CG
AclI 1979 AACGTT AA^CGTT
AocI 3493 CCTCAGG CC^T[ACGT]AGG
BalI 2849 TGGCCA TGG^CCA
BbeI 2617 GGCGCC GGCGC^C
BbsI 2434 GAAGAC GAAGAC(2/6)
BcgI 3278 CGACAACGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 52 CTAG C^TAG
BglII 995 AGATCT A^GATCT
BpuAI 2434 GAAGAC GAAGAC(2/6)
BsaAI 2491 TACGTG [CT]AC^GT[GA]
BsiEI 3194 CGGTCG CG[GA][CT]^CG
BsmBI 2649 CGTCTC CGTCTC(1/5)
Bsp1407I 3380 TGTACA T^GTACA
BspDI 466 ATCGAT AT^CGAT
BsrBI 1764 GAGCGG CCGCTC(-3/-3)
BsrFI 2675 ACCGGC [GA]^CCGG[CT]
BsrGI 3380 TGTACA T^GTACA
BssSI 340 CACGAG CACGAG(-5/-1)
BstAPI 2579 GCACCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1039 TTCGAA TT^CGAA
BstUI 1716 CGCG CG^CG
Bsu36I 3493 CCTCAGG CC^T[ACGT]AGG
Cfr10I 2675 ACCGGC [GA]^CCGG[CT]
ClaI 466 ATCGAT AT^CGAT
DraI 940 TTTAAA TTT^AAA
DraIII 2129 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 3493 CCTCAGG CC^T[ACGT]AGG
EcoRI 613 GAATTC G^AATTC
KasI 2617 GGCGCC G^GCGCC
MaeI 52 CTAG C^TAG
MfeI 854 CAATTG C^AATTG
MluNI 2849 TGGCCA TGG^CCA
MscI 2849 TGGCCA TGG^CCA
MunI 854 CAATTG C^AATTG
MvnI 1716 CGCG CG^CG
NarI 2617 GGCGCC GG^CGCC
NcoI 821 CCATGG C^CATGG
NdeI 728 CATATG CA^TATG
NspV 1039 TTCGAA TT^CGAA
Psp1406I 1979 AACGTT AA^CGTT
SbfI 240 CCTGCAGG CCTGCA^GG
SfoI 2617 GGCGCC GGC^GCC
SfuI 1039 TTCGAA TT^CGAA
SmaI 1817 CCCGGG CCC^GGG
SphI 1871 GCATGC GCATG^C
Sse8387I 240 CCTGCAGG CCTGCA^GG
SspBI 3380 TGTACA T^GTACA
XmaCI 1817 CCCGGG C^CCGGG
XmaI 1817 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AatII 2647 GACGTC GACGT^C
3097 GACGTC
Alw44I 334 GTGCAC G^TGCAC
706 GTGCAC
AlwI 1893 GATCC GGATC(4/5)
1712 GGATC
Aor51HI 100 AGCGCT AGC^GCT
1069 AGCGCT
ApaLI 334 GTGCAC G^TGCAC
706 GTGCAC
BbvCI 636 CCTCAGC CCTCAGC(-5/-2)
3150 CCTCAGC
BsaWI 367 ACCGGA [AT]^CCGG[AT]
2090 ACCGGA
BsgI 2530 CTGCAC GTGCAG(16/14)
1249 GTGCAG
BstXI 183 CCAAACGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1223 CCACCGAGCTGG
BstYI 995 AGATCT [GA]^GATC[CT]
1892 AGATCC
DrdI 115 GACAGCGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2890 GACCTGCCTGTC
Eco47III 100 AGCGCT AGC^GCT
1069 AGCGCT
EcoNI 1487 CCTTGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1779 CCTCACCCAGG
MflI 995 AGATCT [GA]^GATC[CT]
1892 AGATCC
MseI 901 TTAA T^TAA
941 TTAA
PpuMI 1680 GGGACCC [GA]G^G[AT]CC[CT]
2888 GGGACCT
SacI 2749 GAGCTC GAGCT^C
3269 GAGCTC
TfiI 2591 GAATC G^A[AT]TC
3048 GAATC
Tru9I 901 TTAA T^TAA
941 TTAA
XhoII 995 AGATCT [GA]^GATC[CT]
1892 AGATCC
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# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflIII AluI AlwNI ApaI ApoI
AspHI AspI AvaI AvaII BanI
BanII BbvI BcnI BmyI BpmI
BsaHI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspLU11I BspMI BsrDI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
StyI TaqI TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI