Restriction map for
pFN21AB8401
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AgeI AhdI AocI ApaI
AscI AseI AsnI AspEI AspI
AvaI AviII AvrII BbeI BbrPI
BcgI BciVI BcnI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsoBI Bsp1407I BspDI BspEI
BsrBI BsrGI BssHII BssSI Bst1107I
BstBI BstUI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco52I
Eco81I FauI FseI FspI HgaI
Hin1I KasI KspI MluI MroI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SspBI
StuI Tth111I XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 1555 GGTACC G^GTACC
Alw44I 818 GTGCAC G^TGCAC
Aor51HI 1286 AGCGCT AGC^GCT
ApaLI 818 GTGCAC G^TGCAC
Asp718I 1555 GGTACC G^GTACC
BalI 470 TGGCCA TGG^CCA
BamHI 1379 GGATCC G^GATCC
BanI 1555 GGTACC G^G[CT][GA]CC
BanII 725 GAGCTC G[GA]GC[CT]^C
BbvCI 1747 GCTGAGG CCTCAGC(-5/-2)
BclI 639 TGATCA T^GATCA
BsmBI 2315 CGTCTC CGTCTC(1/5)
BspLU11I 1922 ACATGT A^CATGT
BspMI 1439 GCAGGT ACCTGC(4/8)
BsrFI 2770 ACCGGC [GA]^CCGG[CT]
BstEII 2145 GGTCACC G^GT[ACGT]ACC
BstPI 2145 GGTCACC G^GT[ACGT]ACC
Cfr10I 2770 ACCGGC [GA]^CCGG[CT]
DraI 2583 TTTAAA TTT^AAA
Eco47III 1286 AGCGCT AGC^GCT
EcoNI 1077 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2145 GGTCACC G^GT[ACGT]ACC
EcoRV 970 GATATC GAT^ATC
FbaI 639 TGATCA T^GATCA
HaeII 1286 AGCGCT [GA]GCGC^[CT]
HapII 2771 CCGG C^CGG
HpaI 2563 GTTAAC GTT^AAC
HpaII 2771 CCGG C^CGG
KpnI 1555 GGTACC GGTAC^C
MluNI 470 TGGCCA TGG^CCA
MscI 470 TGGCCA TGG^CCA
MspI 2771 CCGG C^CGG
PacI 31 TTAATTAA TTAAT^TAA
PflMI 2799 CCAGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1441 AGGTCCC [GA]G^G[AT]CC[CT]
SacI 725 GAGCTC GAGCT^C
SapI 1060 GAAGAGC GCTCTTC(1/4)
SpeI 2851 ACTAGT A^CTAGT
SspI 2350 AATATT AAT^ATT
SwaI 2582 ATTTAAAT ATTT^AAAT
Van91I 2799 CCAGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AciI 1437 CCGC CCGC(-3/-1)
7 GCGG
AflII 624 CTTAAG C^TTAAG
2811 CTTAAG
AflIII 26 ACACGT A^C[GA][CT]GT
1922 ACATGT
AvaII 1442 GGTCC G^G[AT]CC
2752 GGACC
BbsI 1408 GAAGAC GAAGAC(2/6)
1609 GAAGAC
BfrI 624 CTTAAG C^TTAAG
2811 CTTAAG
BpuAI 1408 GAAGAC GAAGAC(2/6)
1609 GAAGAC
BseRI 2333 CTCCTC GAGGAG(10/8)
1367 GAGGAG
BspHI 939 TCATGA T^CATGA
2337 TCATGA
BstAPI 820 GCACCAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1243 GCAGCAGCTGC
Cac8I 411 GCCAGC GC[ACGT]^[ACGT]GC
2493 GCCTGC
CfoI 1287 GCGC GCG^C
1698 GCGC
DraII 1441 AGGTCCC [GA]G^G[ACGT]CC[CT]
2645 AGGCCCT
EaeI 470 TGGCCA [CT]^GGCC[GA]
2772 CGGCCA
EcoO109I 1441 AGGTCCC [GA]G^G[ACGT]CC[CT]
2645 AGGCCCT
EcoRI 2515 GAATTC G^AATTC
2783 GAATTC
EcoT14I 1403 CCAAGG C^C[AT][AT]GG
1446 CCTTGG
EcoT22I 308 ATGCAT ATGCA^T
575 ATGCAT
HhaI 1287 GCGC GCG^C
1698 GCGC
HinP1I 1287 GCGC G^CGC
1698 GCGC
HindIII 119 AAGCTT A^AGCTT
2186 AAGCTT
MfeI 1460 CAATTG C^AATTG
2152 CAATTG
MunI 1460 CAATTG C^AATTG
2152 CAATTG
NsiI 308 ATGCAT ATGCA^T
575 ATGCAT
NspI 424 GCATGT [GA]CATG^[CT]
1922 ACATGT
PstI 667 CTGCAG CTGCA^G
1021 CTGCAG
RcaI 939 TCATGA T^CATGA
2337 TCATGA
ScaI 628 AGTACT AGT^ACT
948 AGTACT
StyI 1403 CCAAGG C^C[AT][AT]GG
1446 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI Asp700I AspHI BbvI BfaI
BmyI BpmI BsaBI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI BstYI Cfr13I DdeI DpnII
EarI EcoRII Fnu4HI FokI HaeIII
HincII HindII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MnlI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XbaI
XhoII XmnI