Restriction map for
pFN21AE4554
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BbrPI BbvCI BcgI
BglI BglII BlnI BlpI Bpu1102I
BsaBI BsaWI BseAI BseRI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI BstBI
BstEII BstPI BstXI Bsu36I CelII
Cfr10I ClaI CpoI DraII DrdI
Eam1105I Eco47III Eco81I EcoO109I EcoO65I
EcoRI EcoRV FseI FspI HgaI
KpnI MamI MluI MroI NaeI
NdeI NgoMIV NheI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SpeI Sse8387I SspBI StuI SwaI
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AflII 1189 CTTAAG C^TTAAG
AflIII 1812 ACGTGT A^C[GA][CT]GT
AspHI 82 GTGCTC G[AT]GC[AT]^C
AvaII 1622 GGACC G^G[AT]CC
BalI 1035 TGGCCA TGG^CCA
BciVI 334 GGATAC GTATCC(6/5)
BclI 1476 TGATCA T^GATCA
BfrI 1189 CTTAAG C^TTAAG
BpmI 604 CTCCAG CTGGAG(16/14)
BsgI 803 GTGCAG GTGCAG(16/14)
BsiEI 8 CGGCCG CG[GA][CT]^CG
BsiHKAI 82 GTGCTC G[AT]GC[AT]^C
BsiWI 77 CGTACG C^GTACG
BsrDI 1683 CATTGC GCAATG(2/0)
BstAPI 730 GCAAAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 952 CACTACGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 8 CGGCCG C^GGCCG
EclXI 8 CGGCCG C^GGCCG
Eco52I 8 CGGCCG C^GGCCG
EcoNI 1663 CCTGACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1476 TGATCA T^GATCA
HindIII 757 AAGCTT A^AGCTT
HpaI 1851 GTTAAC GTT^AAC
MfeI 1946 CAATTG C^AATTG
MluNI 1035 TGGCCA TGG^CCA
MscI 1035 TGGCCA TGG^CCA
MunI 1946 CAATTG C^AATTG
NotI 7 GCGGCCGC GC^GGCCGC
SfcI 1219 CTACAG C^T[GA][CT]AG
SmlI 1189 CTTAAG C^T[CT][GA]AG
SnaBI 1060 TACGTA TAC^GTA
SphI 1880 GCATGC GCATG^C
XcmI 1927 CCAAAGAATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 1230 GTATAC GT^[AC][GT]AC
1839 GTATAC
AcyI 63 GGCGCC G[GA]^CG[CT]C
493 GGCGCC
AfaI 78 GTAC GT^AC
1415 GTAC
AlwI 1195 GGATC GGATC(4/5)
1759 GGATC
BbeI 63 GGCGCC GGCGC^C
493 GGCGCC
BcnI 67 CCCGG CC^[GC]GG
108 CCGGG
BsaHI 63 GGCGCC G[GA]^CG[CT]C
493 GGCGCC
Bst1107I 1230 GTATAC GTA^TAC
1839 GTATAC
BstYI 1195 GGATCT [GA]^GATC[CT]
1759 GGATCT
BstZ17I 1230 GTATAC GTA^TAC
1839 GTATAC
EcoT14I 400 CCATGG C^C[AT][AT]GG
940 CCATGG
EcoT22I 301 ATGCAT ATGCA^T
1583 ATGCAT
HaeII 63 GGCGCC [GA]GCGC^[CT]
493 GGCGCC
HapII 68 CCGG C^CGG
108 CCGG
Hin1I 63 GGCGCC G[GA]^CG[CT]C
493 GGCGCC
HincII 744 GTTGAC GT[CT]^[GA]AC
1851 GTTAAC
HindII 744 GTTGAC GT[CT]^[GA]AC
1851 GTTAAC
HpaII 68 CCGG C^CGG
108 CCGG
KasI 63 GGCGCC G^GCGCC
493 GGCGCC
KspI 11 CCGCGG CCGC^GG
32 CCGCGG
MflI 1195 GGATCT [GA]^GATC[CT]
1759 GGATCT
MslI 1327 CATGAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1911 CATCCCTGTG
MspI 68 CCGG C^CGG
108 CCGG
NarI 63 GGCGCC GG^CGCC
493 GGCGCC
NciI 67 CCCGG CC^[GC]GG
108 CCGGG
NcoI 400 CCATGG C^CATGG
940 CCATGG
NsiI 301 ATGCAT ATGCA^T
1583 ATGCAT
NspI 299 ACATGC [GA]CATG^[CT]
1880 GCATGC
PvuII 457 CAGCTG CAG^CTG
1273 CAGCTG
RsaI 78 GTAC GT^AC
1415 GTAC
SacII 11 CCGCGG CCGC^GG
32 CCGCGG
SfoI 63 GGCGCC GGC^GCC
493 GGCGCC
StyI 400 CCATGG C^C[AT][AT]GG
940 CCATGG
XhoII 1195 GGATCT [GA]^GATC[CT]
1759 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AcsI AluI ApoI
Asp700I BanI BanII BbsI BbvI
BfaI BmyI BpuAI BsaAI BsaI
BsaJI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DsaI EaeI EarI EcoRII
FauI Fnu4HI FokI HaeIII HhaI
HinP1I HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MspA1I MvaI MvnI
MwoI NdeII NlaIII NlaIV PflMI
PleI Sau3AI Sau96I ScrFI SfaNI
SspI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XmnI