Restriction map for
pFN21AE4420
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# NO CUT ENZYMES ( 133 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsiEI BsiWI BsmAI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstXI BstZ17I
CelII ClaI CpoI DraI EagI
Eam1105I EclXI Eco52I EcoNI EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
MamI MfeI MluI MroI MunI
NaeI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcyI 398 GGCGCC G[GA]^CG[CT]C
AfaI 463 GTAC GT^AC
AocI 493 CCTCAGG CC^T[ACGT]AGG
Aor51HI 446 AGCGCT AGC^GCT
AvaII 516 GGACC G^G[AT]CC
BalI 484 TGGCCA TGG^CCA
BbeI 398 GGCGCC GGCGC^C
BbsI 163 GTCTTC GAAGAC(2/6)
BfaI 694 CTAG C^TAG
BglII 863 AGATCT A^GATCT
BpuAI 163 GTCTTC GAAGAC(2/6)
BsaHI 398 GGCGCC G[GA]^CG[CT]C
BsgI 533 CTGCAC GTGCAG(16/14)
BsmFI 422 GGGAC GGGAC(10/14)
BsrBI 1288 GAGCGG CCGCTC(-3/-3)
BstEII 829 GGTGACC G^GT[ACGT]ACC
BstPI 829 GGTGACC G^GT[ACGT]ACC
BstYI 863 AGATCT [GA]^GATC[CT]
Bsu36I 493 CCTCAGG CC^T[ACGT]AGG
DrdI 148 GACGTGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 446 AGCGCT AGC^GCT
Eco81I 493 CCTCAGG CC^T[ACGT]AGG
EcoO65I 829 GGTGACC G^GT[ACGT]ACC
HgaI 579 GCGTC GACGC(5/10)
Hin1I 398 GGCGCC G[GA]^CG[CT]C
HinfI 991 GAATC G^A[ACGT]TC
KasI 398 GGCGCC G^GCGCC
KspI 198 CCGCGG CCGC^GG
MaeI 694 CTAG C^TAG
MflI 863 AGATCT [GA]^GATC[CT]
MluNI 484 TGGCCA TGG^CCA
MscI 484 TGGCCA TGG^CCA
MseI 1145 TTAA T^TAA
NarI 398 GGCGCC GG^CGCC
NcoI 1095 CCATGG C^CATGG
PflMI 98 CCACCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1071 CTGCAG CTGCA^G
RsaI 463 GTAC GT^AC
SacII 198 CCGCGG CCGC^GG
SapI 1293 GAAGAGC GCTCTTC(1/4)
SfoI 398 GGCGCC GGC^GCC
TfiI 991 GAATC G^A[AT]TC
Tru9I 1145 TTAA T^TAA
Tsp509I 216 AATT ^AATT
Van91I 98 CCACCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 863 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
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AccII 199 CGCG CG^CG
586 CGCG
BspMI 11 ACCTGC ACCTGC(4/8)
351 ACCTGC
BstUI 199 CGCG CG^CG
586 CGCG
DpnII 864 GATC ^GATC
1222 GATC
EarI 31 CTCTTC CTCTTC(1/4)
1293 GAAGAG
HaeII 398 GGCGCC [GA]GCGC^[CT]
446 AGCGCT
Ksp632I 31 CTCTTC CTCTTC(1/4)
1293 GAAGAG
MaeII 149 ACGT A^CGT
1049 ACGT
MboI 864 GATC ^GATC
1222 GATC
MslI 735 CATCTACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1277 CACCGTGGTG
MvnI 199 CGCG CG^CG
586 CGCG
NdeII 864 GATC ^GATC
1222 GATC
NspI 740 ACATGC [GA]CATG^[CT]
1298 GCATGT
Sau3AI 864 GATC ^GATC
1222 GATC
SfcI 222 CTATAG C^T[GA][CT]AG
1071 CTGCAG
XcmI 936 CCAGGGGGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1167 CCAGCTCACGCCTGG
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# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AluI ApaI AspHI BanI
BanII BbvI BglI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr10I
Cfr13I DdeI DraII DraIII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeIII
MboII MnlI MspA1I MspI MvaI
MwoI NlaIII NlaIV PvuII Sau96I
ScrFI SfaNI StyI TaqI TseI
Tsp45I TspRI TthHB8I