Restriction map for
pFN21AE4332
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AhdI Alw44I AlwI AlwNI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AviII AvrII BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BpmI BpuAI
BsaAI BsaBI BsaHI BseAI BsiEI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BsrBI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstYI BstZ17I
CfoI ClaI CpoI DpnII DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoRI EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HindIII
HpaI KasI KpnI KspI MaeI
MamI MboI MfeI MflI MluI
MroI MspA1I MunI MvnI NaeI
NarI NcoI NdeII NgoMIV NheI
NotI NruI NsiI NspV PacI
PflMI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SapI Sau3AI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspI StuI SwaI Tsp509I Van91I
XbaI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AflIII 467 ACATGT A^C[GA][CT]GT
AgeI 712 ACCGGT A^CCGGT
AocI 892 CCTCAGG CC^T[ACGT]AGG
AvaI 633 CTCGAG C^[CT]CG[GA]G
BalI 58 TGGCCA TGG^CCA
BlpI 1015 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1015 GCTGAGC GC^T[ACGT]AGC
BsaI 237 GGTCTC GGTCTC(1/5)
BsaWI 712 ACCGGT [AT]^CCGG[AT]
BsgI 178 CTGCAC GTGCAG(16/14)
BsoBI 633 CTCGAG C^[CT]CG[GA]G
Bsp1407I 995 TGTACA T^GTACA
BspLU11I 467 ACATGT A^CATGT
BspMI 728 ACCTGC ACCTGC(4/8)
BsrDI 141 GCAATG GCAATG(2/0)
BsrFI 712 ACCGGT [GA]^CCGG[CT]
BsrGI 995 TGTACA T^GTACA
BsrI 341 CCAGT ACTGG(1/-1)
BstEII 715 GGTAACC G^GT[ACGT]ACC
BstPI 715 GGTAACC G^GT[ACGT]ACC
Bsu36I 892 CCTCAGG CC^T[ACGT]AGG
Cac8I 843 GCCTGC GC[ACGT]^[ACGT]GC
CelII 1015 GCTGAGC GC^T[ACGT]AGC
Cfr10I 712 ACCGGT [GA]^CCGG[CT]
EaeI 58 TGGCCA [CT]^GGCC[GA]
Eco81I 892 CCTCAGG CC^T[ACGT]AGG
EcoO65I 715 GGTAACC G^GT[ACGT]ACC
EcoRV 985 GATATC GAT^ATC
HincII 529 GTCAAC GT[CT]^[GA]AC
HindII 529 GTCAAC GT[CT]^[GA]AC
MaeII 504 ACGT A^CGT
MluNI 58 TGGCCA TGG^CCA
MscI 58 TGGCCA TGG^CCA
MseI 197 TTAA T^TAA
NdeI 905 CATATG CA^TATG
PaeR7I 633 CTCGAG C^TCGAG
PinAI 712 ACCGGT A^CCGGT
SacI 456 GAGCTC GAGCT^C
SbfI 844 CCTGCAGG CCTGCA^GG
SmlI 633 CTCGAG C^T[CT][GA]AG
Sse8387I 844 CCTGCAGG CCTGCA^GG
SspBI 995 TGTACA T^GTACA
TaqI 634 TCGA T^CGA
TfiI 918 GAATC G^A[AT]TC
Tru9I 197 TTAA T^TAA
TthHB8I 634 TCGA T^CGA
XcmI 341 CCAGTGACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 633 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AfaI 471 GTAC GT^AC
996 GTAC
Asp700I 80 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
665 GAAACCCTTC
AspHI 223 GAGCAC G[AT]GC[AT]^C
456 GAGCTC
AspI 355 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
435 GACCTTGTC
BcnI 403 CCGGG CC^[GC]GG
452 CCCGG
BseRI 775 CTCCTC GAGGAG(10/8)
778 CTCCTC
BsiHKAI 223 GAGCAC G[AT]GC[AT]^C
456 GAGCTC
BsmAI 127 GAGAC GTCTC(1/5)
238 GTCTC
BstXI 410 CCACGGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
616 CCATACGAGTGG
DsaI 410 CCACGG C^C[GA][CT]GG
573 CCACGG
EcoT14I 242 CCAAGG C^C[AT][AT]GG
524 CCAAGG
MwoI 449 GCTCCCGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
800 GCTGGTGCTGC
NciI 403 CCGGG CC^[GC]GG
452 CCCGG
NspI 467 ACATGT [GA]CATG^[CT]
732 GCATGT
PflFI 355 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
435 GACCTTGTC
RsaI 471 GTAC GT^AC
996 GTAC
StyI 242 CCAAGG C^C[AT][AT]GG
524 CCAAGG
Tth111I 355 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
435 GACCTTGTC
XmnI 80 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
665 GAAACCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AluI ApaI AvaII BanII BbvI
BmyI BsaJI BsiYI BslI BsmFI
Bsp1286I BssKI BstF5I BstNI Cfr13I
DdeI DraII EarI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeIII
MboII MnlI MslI MspI MvaI
NlaIII NlaIV PleI PpuMI PstI
Sau96I ScrFI SfaNI SfcI TseI
Tsp45I TspRI