##################################
# NO CUT ENZYMES (  202 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspHI       AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BcnI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        BpmI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaJI       BsaWI       BseAI       BseRI       
BsiEI       BsiHKAI     BsiWI       BsiYI       BslI        
BsmBI       BsmFI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BsrI        BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstF5I      BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        DsaI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        FokI        FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HpaI        HpaII       HphI        
KasI        KpnI        KspI        MaeII       MaeIII      
MamI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MslI        MspA1I      MspI        
MunI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NlaIV       NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SbfI        ScaI        SexAI       SfaNI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        Sse8387I    SspBI       StuI        
StyI        SwaI        Tsp45I      TspRI       Tth111I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AciI          445  CCGC                 CCGC(-3/-1)
Asp700I       145  GAAGTCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbsI          595  GAAGAC               GAAGAC(2/6)
BbvI          493  GCTGC                GCAGC(8/12)
BfaI          367  CTAG                 C^TAG
BpuAI         595  GAAGAC               GAAGAC(2/6)
BsaI          392  GAGACC               GGTCTC(1/5)
BsgI           23  GTGCAG               GTGCAG(16/14)
BsmAI         392  GAGAC                GTCTC(1/5)
BssKI         362  CCAGG                ^CC[ACGT]GG
BstNI         362  CCAGG                CC^[AT]GG
Cfr13I        536  GGGCC                G^G[ACGT]CC
EaeI          336  TGGCCG               [CT]^GGCC[GA]
EcoRI         587  GAATTC               G^AATTC
EcoRII        362  CCAGG                ^CC[AT]GG
Fnu4HI        493  GCTGC                GC^[ACGT]GC
HincII        178  GTTGAC               GT[CT]^[GA]AC
HindII        178  GTTGAC               GT[CT]^[GA]AC
HindIII       156  AAGCTT               A^AGCTT
HinfI         268  GAATC                G^A[ACGT]TC
ItaI          493  GCTGC                GC^[ACGT]GC
MaeI          367  CTAG                 C^TAG
MseI           85  TTAA                 T^TAA
MvaI          362  CCAGG                CC^[AT]GG
NheI          366  GCTAGC               G^CTAGC
NspI          297  GCATGC               [GA]CATG^[CT]
Sau96I        536  GGGCC                G^G[ACGT]CC
ScrFI         362  CCAGG                CC^[ACGT]GG
SfcI          307  CTACAG               C^T[GA][CT]AG
SphI          297  GCATGC               GCATG^C
SspI           54  AATATT               AAT^ATT
TaqI          390  TCGA                 T^CGA
TfiI          268  GAATC                G^A[AT]TC
Tru9I          85  TTAA                 T^TAA
TseI          493  GCTGC                G^C[AT]GC
TthHB8I       390  TCGA                 T^CGA
XmnI          145  GAAGTCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

AcsI          547  AAATTT               [GA]^AATT[CT]
              587  GAATTC              
AlwI          442  GATCC                GGATC(4/5)
              191  GGATC               
ApoI          547  AAATTT               [GA]^AATT[CT]
              587  GAATTC              
DpnII         192  GATC                 ^GATC
              442  GATC                
EarI          233  CTCTTC               CTCTTC(1/4)
              352  GAAGAG              
HaeIII        337  GGCC                 GG^CC
              537  GGCC                
Ksp632I       233  CTCTTC               CTCTTC(1/4)
              352  GAAGAG              
MboI          192  GATC                 ^GATC
              442  GATC                
NdeII         192  GATC                 ^GATC
              442  GATC                
SapI          352  GAAGAGC              GCTCTTC(1/4)
              232  GCTCTTC             
Sau3AI        192  GATC                 ^GATC
              442  GATC                


#########################################
# 3 OR MORE CUT ENZYMES (    8 enzymes) #
#########################################

AluI        Cac8I       DdeI        MboII       MnlI        
MwoI        NlaIII      Tsp509I