Restriction map for
pFN21AE4510
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AflIII Alw44I Aor51HI
ApaLI AscI Asp718I AspI AvaI
AviII AvrII BamHI BanI BbeI
BbrPI BbvCI BcgI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsgI BsiWI BsmBI
BsoBI BspDI BspLU11I BsrBI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I CelII ClaI CpoI
DraIII DrdI EagI EclXI Eco47III
Eco52I EcoO65I EcoRV EcoT22I FseI
FspI HaeII HgaI Hin1I HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MunI MvnI NaeI
NarI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PstI PvuI PvuII
RsrII SacII SalI SbfI ScaI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccIII 439 TCCGGA T^CCGGA
AgeI 1472 ACCGGT A^CCGGT
AhdI 1147 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 54 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 831 CCTGAGG CC^T[ACGT]AGG
ApaI 1422 GGGCCC GGGCC^C
AseI 1225 ATTAAT AT^TAAT
AsnI 1225 ATTAAT AT^TAAT
Asp700I 909 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1147 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 859 GAGCTC G[AT]GC[AT]^C
BbsI 1207 GAAGAC GAAGAC(2/6)
BclI 632 TGATCA T^GATCA
BcnI 666 CCGGG CC^[GC]GG
BfaI 155 CTAG C^TAG
BpuAI 1207 GAAGAC GAAGAC(2/6)
BseAI 439 TCCGGA T^CCGGA
BsiEI 1474 CGGTCG CG[GA][CT]^CG
BsiHKAI 859 GAGCTC G[AT]GC[AT]^C
Bsp1407I 764 TGTACA T^GTACA
BspEI 439 TCCGGA T^CCGGA
BspHI 629 TCATGA T^CATGA
BspMI 85 ACCTGC ACCTGC(4/8)
BsrFI 1472 ACCGGT [GA]^CCGG[CT]
BsrGI 764 TGTACA T^GTACA
BssSI 1496 CACGAG CACGAG(-5/-1)
Bsu36I 831 CCTGAGG CC^T[ACGT]AGG
CfoI 1248 GCGC GCG^C
Cfr10I 1472 ACCGGT [GA]^CCGG[CT]
DraII 113 GGGGCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 1147 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 831 CCTGAGG CC^T[ACGT]AGG
EcoNI 47 CCTCCATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 113 GGGGCCT [GA]G^G[ACGT]CC[CT]
FauI 1157 CCCGC CCCGC(4/6)
FbaI 632 TGATCA T^GATCA
HhaI 1248 GCGC GCG^C
HinP1I 1248 GCGC G^CGC
HphI 657 GGTGA GGTGA(8/7)
MaeI 155 CTAG C^TAG
MaeII 905 ACGT A^CGT
MroI 439 TCCGGA T^CCGGA
MslI 1095 CATAGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1158 CCGCTG C[AC]G^C[GT]G
NciI 666 CCGGG CC^[GC]GG
NheI 154 GCTAGC G^CTAGC
NspI 695 ACATGC [GA]CATG^[CT]
PinAI 1472 ACCGGT A^CCGGT
PshBI 1225 ATTAAT AT^TAAT
RcaI 629 TCATGA T^CATGA
SacI 859 GAGCTC GAGCT^C
SapI 96 GAAGAGC GCTCTTC(1/4)
SexAI 534 ACCAGGT A^CC[AT]GGT
SspBI 764 TGTACA T^GTACA
TaqI 711 TCGA T^CGA
Tsp45I 706 GTCAC ^GT[GC]AC
TthHB8I 711 TCGA T^CGA
XcmI 1364 CCAAATTGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 909 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AciI 612 CCGC CCGC(-3/-1)
1158 CCGC
AlwI 258 GATCC GGATC(4/5)
1360 GATCC
AvaII 947 GGACC G^G[AT]CC
1470 GGACC
BciVI 328 GTATCC GTATCC(6/5)
453 GTATCC
BpmI 1150 CTCCAG CTGGAG(16/14)
76 CTGGAG
BsaI 922 GAGACC GGTCTC(1/5)
1270 GAGACC
BsaWI 439 TCCGGA [AT]^CCGG[AT]
1472 ACCGGT
BsmI 780 GAATGC GAATGC(1/-1)
872 GCATTC
BsrDI 273 CATTGC GCAATG(2/0)
101 GCAATG
BstXI 1116 CCAGTCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1426 CCAGGGCAGTGG
BstYI 257 AGATCC [GA]^GATC[CT]
1359 AGATCC
Cac8I 154 GCTAGC GC[ACGT]^[ACGT]GC
868 GCAAGC
DdeI 607 CTCAG C^T[ACGT]AG
832 CTGAG
DsaI 1342 CCATGG C^C[GA][CT]GG
1373 CCATGG
EcoRI 579 GAATTC G^AATTC
913 GAATTC
MaeIII 379 GTTAC ^GT[ACGT]AC
706 GTCAC
MflI 257 AGATCC [GA]^GATC[CT]
1359 AGATCC
NcoI 1342 CCATGG C^CATGG
1373 CCATGG
SfaNI 421 GATGC GCATC(5/9)
813 GATGC
SmlI 727 CTCAAG C^T[CT][GA]AG
1081 CTTGAG
TfiI 406 GAATC G^A[AT]TC
788 GATTC
XhoII 257 AGATCC [GA]^GATC[CT]
1359 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AcsI AfaI AluI ApoI BalI
BanII BbvI BmyI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cfr13I
DpnII DraI EaeI EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HincII HindII HinfI HpaII ItaI
Ksp632I MboI MboII MluNI MnlI
MscI MseI MspI MvaI MwoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI StyI Tru9I
TseI Tsp509I TspRI