Restriction map for
pFN21AE3930
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# NO CUT ENZYMES ( 115 enzymes) #
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AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BbrPI BbsI BcgI
BciVI BclI BfrI BglII BlnI
BpuAI BsaAI BsaBI BsiWI BsmBI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrDI BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI DraI
DraIII Eam1105I Eco47III EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI HincII HindII HindIII HpaI
KpnI MaeII MamI MfeI MluI
MluNI MscI MseI MunI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI SalI SapI
SbfI ScaI SexAI SfiI SfuI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I Tsp509I
Tth111I Van91I XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccIII 881 TCCGGA T^CCGGA
AfaI 84 GTAC GT^AC
AocI 664 CCTGAGG CC^T[ACGT]AGG
AscI 126 GGCGCGCC GG^CGCGCC
AspHI 187 GAGCTC G[AT]GC[AT]^C
BamHI 965 GGATCC G^GATCC
BbvCI 1184 CCTCAGC CCTCAGC(-5/-2)
BglI 1192 GCCCCAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 466 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 466 GCTGAGC GC^T[ACGT]AGC
BsaWI 881 TCCGGA [AT]^CCGG[AT]
BseAI 881 TCCGGA T^CCGGA
BsgI 1085 CTGCAC GTGCAG(16/14)
BsiHKAI 187 GAGCTC G[AT]GC[AT]^C
BspEI 881 TCCGGA T^CCGGA
BsrBI 760 GAGCGG CCGCTC(-3/-3)
BsrI 804 ACTGG ACTGG(1/-1)
BstAPI 255 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 664 CCTGAGG CC^T[ACGT]AGG
CelII 466 GCTGAGC GC^T[ACGT]AGC
CpoI 359 CGGACCG CG^G[AT]CCG
DrdI 835 GACCACCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1108 GAAGAG CTCTTC(1/4)
Eco81I 664 CCTGAGG CC^T[ACGT]AGG
EcoNI 789 CCTGCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 961 CCATGG C^C[AT][AT]GG
HphI 459 GGTGA GGTGA(8/7)
Ksp632I 1108 GAAGAG CTCTTC(1/4)
MaeIII 355 GTCAC ^GT[ACGT]AC
MroI 881 TCCGGA T^CCGGA
MslI 867 CATGAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 961 CCATGG C^CATGG
PaeR7I 859 CTCGAG C^TCGAG
RsaI 84 GTAC GT^AC
RsrII 359 CGGACCG CG^G[AT]CCG
SacI 187 GAGCTC GAGCT^C
SfaNI 64 GCATC GCATC(5/9)
SgrAI 639 CGCCGGCG C[GA]^CCGG[CT]G
StyI 961 CCATGG C^C[AT][AT]GG
TaqI 860 TCGA T^CGA
TfiI 1057 GAATC G^A[AT]TC
Tsp45I 355 GTCAC ^GT[GC]AC
TthHB8I 860 TCGA T^CGA
XhoI 859 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 18 enzymes) #
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ApaI 98 GGGCCC GGGCC^C
211 GGGCCC
BpmI 1130 CTCCAG CTGGAG(16/14)
184 CTGGAG
BspMI 398 ACCTGC ACCTGC(4/8)
231 GCAGGT
BsrFI 246 GCCGGC [GA]^CCGG[CT]
640 GCCGGC
BstXI 537 CCAGCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
866 CCATGAAGGTGG
Cfr10I 246 GCCGGC [GA]^CCGG[CT]
640 GCCGGC
EagI 68 CGGCCG C^GGCCG
152 CGGCCG
EclXI 68 CGGCCG C^GGCCG
152 CGGCCG
Eco52I 68 CGGCCG C^GGCCG
152 CGGCCG
KspI 53 CCGCGG CCGC^GG
71 CCGCGG
NaeI 246 GCCGGC GCC^GGC
640 GCCGGC
NgoMIV 246 GCCGGC G^CCGGC
640 GCCGGC
PpuMI 668 AGGACCC [GA]G^G[AT]CC[CT]
842 GGGTCCC
PvuII 439 CAGCTG CAG^CTG
502 CAGCTG
SacII 53 CCGCGG CCGC^GG
71 CCGCGG
SmaI 22 CCCGGG CCC^GGG
601 CCCGGG
XmaCI 22 CCCGGG C^CCGGG
601 CCCGGG
XmaI 22 CCCGGG C^CCGGG
601 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AlwNI AvaI AvaII BanI BanII
BbeI BbvI BcnI BfaI BmyI
BsaHI BsaI BsaJI BseRI BsiEI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BssHII BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII ItaI KasI
MaeI MboI MboII MflI MnlI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
PleI PstI Sau3AI Sau96I ScrFI
SfcI SfoI SmlI TseI TspRI
XhoII