Restriction map for
pFN21AE2403
##################################
# NO CUT ENZYMES ( 181 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI AlwNI AocI Aor51HI ApaI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BcnI BfrI BlnI BlpI Bpu1102I
BpuAI BsaBI BsaHI BsaI BsaJI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MseI MslI MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI TaqI
Tru9I Tsp509I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Alw44I 189 GTGCAC G^TGCAC
ApaLI 189 GTGCAC G^TGCAC
AspHI 189 GTGCAC G[AT]GC[AT]^C
BamHI 63 GGATCC G^GATCC
BclI 144 TGATCA T^GATCA
BfaI 194 CTAG C^TAG
BglI 69 GCCAAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 609 AGATCT A^GATCT
BmyI 189 GTGCAC G[AGT]GC[ACT]^C
BsaAI 496 TACGTG [CT]AC^GT[GA]
BsiHKAI 189 GTGCAC G[AT]GC[AT]^C
BsiYI 359 CCCATAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 359 CCCATAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 189 GTGCAC G[AGT]GC[ACT]^C
BspHI 132 TCATGA T^CATGA
BspMI 390 GCAGGT ACCTGC(4/8)
BsrDI 615 CATTGC GCAATG(2/0)
BssKI 285 CCAGG ^CC[ACGT]GG
BstAPI 205 GCAGAGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 491 GGATG GGATG(2/0)
BstNI 285 CCAGG CC^[AT]GG
Cac8I 74 GCTGGC GC[ACGT]^[ACGT]GC
DrdI 4 GACCCCGTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRII 285 CCAGG ^CC[AT]GG
FbaI 144 TGATCA T^GATCA
FokI 491 GGATG GGATG(9/13)
MaeI 194 CTAG C^TAG
MspA1I 290 CAGCTG C[AC]G^C[GT]G
MvaI 285 CCAGG CC^[AT]GG
PstI 559 CTGCAG CTGCA^G
PvuII 290 CAGCTG CAG^CTG
RcaI 132 TCATGA T^CATGA
ScaI 110 AGTACT AGT^ACT
ScrFI 285 CCAGG CC^[ACGT]GG
SfcI 559 CTGCAG C^T[GA][CT]AG
SmlI 15 CTTGAG C^T[CT][GA]AG
StuI 515 AGGCCT AGG^CCT
Tsp45I 172 GTCAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 32 GTCTAC GT^[AC][GT]AC
319 GTCTAC
AciI 67 CCGC CCGC(-3/-1)
39 GCGG
AfaI 111 GTAC GT^AC
495 GTAC
AlwI 64 GATCC GGATC(4/5)
63 GGATC
AvaII 3 GGACC G^G[AT]CC
234 GGACC
BsmAI 431 GAGAC GTCTC(1/5)
52 GTCTC
BstXI 87 CCATATTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
285 CCAGGCAGCTGG
BstYI 63 GGATCC [GA]^GATC[CT]
609 AGATCT
Cfr13I 3 GGACC G^G[ACGT]CC
234 GGACC
EarI 451 GAAGAG CTCTTC(1/4)
591 GAAGAG
HaeIII 441 GGCC GG^CC
516 GGCC
HinfI 355 GATTC G^A[ACGT]TC
550 GAATC
HphI 173 TCACC GGTGA(8/7)
569 TCACC
Ksp632I 451 GAAGAG CTCTTC(1/4)
591 GAAGAG
MaeII 149 ACGT A^CGT
497 ACGT
MboII 451 GAAGA GAAGA(8/7)
591 GAAGA
MflI 63 GGATCC [GA]^GATC[CT]
609 AGATCT
RsaI 111 GTAC GT^AC
495 GTAC
Sau96I 3 GGACC G^G[ACGT]CC
234 GGACC
TfiI 355 GATTC G^A[AT]TC
550 GAATC
XhoII 63 GGATCC [GA]^GATC[CT]
609 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AluI BbvI BpmI BsrI DdeI
DpnII Fnu4HI ItaI MaeIII MboI
MnlI MwoI NdeII NlaIII NlaIV
Sau3AI TseI TspRI